Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,587,370 C→T T143I (ACT→ATT) 
*248* (TAG→TAA
yhhA →
ugpQ ←
DUF2756 family protein
glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,587,3700CT100.0% 182.1 / NA 56T143I (ACT→ATT) 
*248* (TAG→TAA
yhhA
ugpQ
DUF2756 family protein
glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/27);  total (29/27)

CACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCG  >  NC_000913/3587236‑3587499
                                                                                                                                      |                                                                                                                                 
cacCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                                               <  1:280708/139‑1 (MQ=255)
cacCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                                               <  1:1601058/139‑1 (MQ=255)
     aCGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGcc                                                                                                                          >  2:1507910/1‑139 (MQ=255)
          gggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGTCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTaaa                                                                                                                     >  1:982921/1‑139 (MQ=255)
          gggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTaaa                                                                                                                     >  2:69938/1‑139 (MQ=255)
           ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTg                                                                                                                              >  1:841647/1‑129 (MQ=255)
           ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTg                                                                                                                              <  2:841647/129‑1 (MQ=255)
           ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAg                                                                                                                    >  1:228362/1‑139 (MQ=255)
             aTGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGtt                                                                                                                  <  2:92117/139‑1 (MQ=255)
                    aCAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCa                                                                                                           <  2:601288/139‑1 (MQ=255)
                        cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCa                                                                                                       >  2:209018/1‑139 (MQ=255)
                        cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCa                                                                                                       >  1:423064/1‑139 (MQ=255)
                          aCCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACg                                                                                                     >  2:82642/1‑139 (MQ=255)
                          aCCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACg                                                                                                     >  2:330633/1‑139 (MQ=255)
                               aaTCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                                                                                <  1:330633/139‑1 (MQ=255)
                               aaTCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                                                                                <  2:228362/139‑1 (MQ=255)
                                           tCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACa                                                                                    >  1:823512/1‑139 (MQ=255)
                                            cGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAg                                                                                   >  2:245347/1‑139 (MQ=255)
                                                      gcagcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCca                                                                         <  1:245347/139‑1 (MQ=255)
                                                       cagcaTATGCTGCCGGAGTGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCcac                                                                        >  2:174945/1‑139 (MQ=255)
                                                       cagcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCcac                                                                        >  2:622304/1‑139 (MQ=255)
                                                            tatGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACAcccc                                                                   >  2:1553192/1‑139 (MQ=255)
                                                             atGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCa                                                                  >  1:1492225/1‑139 (MQ=255)
                                                                 tgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                                               >  2:598077/1‑74 (MQ=255)
                                                                 tgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                                               <  1:598077/74‑1 (MQ=255)
                                                                   ccGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                            <  1:490847/139‑1 (MQ=255)
                                                                        gaggagaAACGGCGACATGCTGAAGCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAAct                                                       >  1:1092102/1‑139 (MQ=255)
                                                                               aaCGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCtgctgc                                                >  2:1378921/1‑139 (MQ=255)
                                                                                aCGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcg                                               <  1:82642/139‑1 (MQ=255)
                                                                                  ggCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcg                                             <  1:622304/139‑1 (MQ=255)
                                                                                   gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcgc                                            <  1:562336/139‑1 (MQ=255)
                                                                                     gACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTg                                          <  1:1367521/139‑1 (MQ=255)
                                                                                        aTGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTgggg                                       <  1:174945/139‑1 (MQ=255)
                                                                                           cTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGttt                                    <  1:979809/139‑1 (MQ=255)
                                                                                            tGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTa                                   >  2:1145091/1‑139 (MQ=255)
                                                                                                tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                               <  1:279680/139‑1 (MQ=255)
                                                                                                tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                               <  2:823512/139‑1 (MQ=255)
                                                                                                    cccAGCACGCCGCAGCCTGACATTCCGTTGAGAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                           >  1:483150/1‑139 (MQ=255)
                                                                                                    cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                           >  2:451269/1‑139 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGt                                                                                                                        >  1:1046106/1‑42 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGt                                                                                                                        <  2:1046106/42‑1 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTg                                          <  1:501848/120‑1 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTg                                          >  2:501848/1‑120 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                               >  2:1542686/1‑131 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                               <  1:1542686/131‑1 (MQ=255)
                                                                                                        gCACGCCGCAGCCTGACATTCCGCTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACCCTGCTGCGCGCTGGGGTTTATTGACGGTATaa                       >  2:571743/1‑139 (MQ=255)
                                                                                                              cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCca                                                                         <  1:126356/83‑1 (MQ=255)
                                                                                                              cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCca                                                                         >  2:126356/1‑83 (MQ=255)
                                                                                                              cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGa                 >  2:668750/1‑139 (MQ=255)
                                                                                                              cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGa                 >  1:1558942/1‑139 (MQ=255)
                                                                                                                           tCCGTTGAGAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  2:483150/139‑1 (MQ=255)
                                                                                                                           tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  1:1553192/139‑1 (MQ=255)
                                                                                                                           tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  1:451269/139‑1 (MQ=255)
                                                                                                                           tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  1:668750/139‑1 (MQ=255)
                                                                                                                           tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  1:1378921/139‑1 (MQ=255)
                                                                                                                           tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  1:1145091/139‑1 (MQ=255)
                                                                                                                           tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg    <  2:982921/139‑1 (MQ=255)
                                                                                                                             cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCgg                                                                                        <  1:1421106/53‑1 (MQ=255)
                                                                                                                             cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCgg                                                                                        >  2:1421106/1‑53 (MQ=255)
                                                                                                                             cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCg  >  2:455539/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                 
CACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCG  >  NC_000913/3587236‑3587499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: