| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,775,526 | G→A | S368N (AGC→AAC) | mtlD → | mannitol‑1‑phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,775,526 | 0 | G | A | 98.4% | 195.4 / ‑4.6 | 62 | S368N (AGC→AAC) | mtlD | mannitol‑1‑phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (25/36); total (25/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAACCCAGGCCTTTGAAAA > NC_000913/3775391‑3775660 | aCCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGa > 2:1328906/1‑139 (MQ=255)aCCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTCATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGa > 1:1151411/1‑139 (MQ=255) ggcttGAAGGGATTGCCGCTGCGATGTGCTTCTTGTGGGTAGAGGATCCGCAGGCTCAGGAACTGGTGGGGGCGATCGCGGACAAAGGTCCTCAGGCGGCGCTGGCCCAGATTTCCGGTGTTGATGCCAACTGCGTGGt < 1:216393/136‑1 (MQ=255) cTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAc < 2:1529081/139‑1 (MQ=255) tGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAcc < 2:395148/139‑1 (MQ=255) tGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAcc < 2:52392/139‑1 (MQ=255) tGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTta < 1:351114/139‑1 (MQ=255) ttCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAcc < 2:1468424/129‑1 (MQ=255) ttCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAcc > 1:1468424/1‑129 (MQ=255) ttCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg < 2:644759/139‑1 (MQ=255) ttCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg < 1:917906/139‑1 (MQ=255) ttCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg < 1:1271368/139‑1 (MQ=255) ccGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAcc > 1:1535236/1‑127 (MQ=255) ccGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAcc < 2:1535236/127‑1 (MQ=255) cGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAgcaa < 2:769270/139‑1 (MQ=255) cGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAgcaa < 1:1161607/139‑1 (MQ=255) cAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg > 1:990584/1‑134 (MQ=255) cAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg > 1:1287864/1‑134 (MQ=255) cAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg < 2:1287864/134‑1 (MQ=255) cAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg < 2:990584/134‑1 (MQ=255) gTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAgcaatgca > 2:417254/1‑139 (MQ=255) gatgatCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATcc < 2:427939/105‑1 (MQ=255) gatgatCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATcc > 1:427939/1‑105 (MQ=255) gatgatCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCaataat > 2:1209646/1‑139 (MQ=255) ccGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAgg > 2:1474117/1‑91 (MQ=255) ccGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAgg < 1:1474117/91‑1 (MQ=255) ccGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTtata > 2:1377423/1‑118 (MQ=255) ccGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTtata < 1:1377423/118‑1 (MQ=255) gCAGGCTCAGGAACTGGCAGCACTGAACGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGAcc < 2:449261/139‑1 (MQ=255) aGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg < 1:94053/117‑1 (MQ=255) aGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAg > 2:94053/1‑117 (MQ=255) aGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGActgt < 2:1151411/139‑4 (MQ=255) ggCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAgg > 1:1050438/1‑139 (MQ=255) gCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGgc < 1:1100865/139‑1 (MQ=255) ggAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAgg > 2:790303/1‑139 (MQ=255) gAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGa < 1:1328906/139‑1 (MQ=255) gcaCTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTgcg > 2:698717/1‑139 (MQ=255) caCTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTgcgc < 1:790303/139‑1 (MQ=255) cTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGccc < 2:579775/139‑1 (MQ=255) gATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATcc > 2:837520/1‑73 (MQ=255) gATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATcc < 1:837520/73‑1 (MQ=255) tCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAAt < 1:279180/139‑1 (MQ=255) cAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGAt > 1:1286283/1‑139 (MQ=255) aaaGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGAtt < 2:832364/139‑1 (MQ=255) aaGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATaa < 1:45050/132‑1 (MQ=255) aaGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATaa > 2:45050/1‑132 (MQ=255) aaGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTg > 2:218978/1‑139 (MQ=255) cAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGatat > 1:218938/1‑139 (MQ=255) aGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATg < 2:1286283/139‑1 (MQ=255) ggcgCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAAtggt < 1:1248502/86‑1 (MQ=255) ggcgCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAAtggt > 2:1248502/1‑86 (MQ=255) ggcgCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGa > 2:479241/1‑123 (MQ=255) ggcgCTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGa < 1:479241/123‑1 (MQ=255) gcgcTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGc < 2:384569/139‑1 (MQ=255) gcgcTGGCACAGATTTCCGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGc < 1:698717/139‑1 (MQ=255) gATTTCCGGTCCTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAAc < 2:1050438/139‑1 (MQ=255) ccGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAAGCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAAcc > 2:1053331/1‑139 (MQ=255) ccGGTCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAAcc < 1:417254/139‑1 (MQ=255) tCTTGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAACCCAgg < 2:218938/139‑1 (MQ=255) ttGATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAACCCAGGcc > 1:923278/1‑139 (MQ=255) gATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAg > 1:208665/1‑68 (MQ=255) gATGCCAACAACGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAg < 2:208665/68‑1 (MQ=255) aacaacGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAACCCAGGCCTTTGaaaa < 2:923278/139‑1 (MQ=255) | ACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAATGGAACAAACCCAGGCCTTTGAAAA > NC_000913/3775391‑3775660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |