Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,017	G→A	*397* (TAG→TAA)	lldD →	L‑lactate dehydrogenase, FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,017	0	G	A	100.0%	118.8 / NA	37	*397* (TAG→TAA)	lldD	L‑lactate dehydrogenase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/24);  total (13/24)

AGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC  >  NC_000913/3780894‑3781145                                                                                                                            |                                                                                                                                 aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                   <  1:1456142/139‑1 (MQ=255) aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                   <  1:815808/139‑1 (MQ=255)      cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                   <  1:1601545/134‑1 (MQ=255)      cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                   >  2:1601545/1‑134 (MQ=255)             gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACTCTAACCct                                                                                                       <  2:1046065/139‑1 (MQ=255)               tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                     <  1:1503434/139‑1 (MQ=255)                                     cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                               <  1:1308945/139‑1 (MQ=255)                                     cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                               <  2:1437212/139‑1 (MQ=255)                                        aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                            <  1:1489672/139‑1 (MQ=255)                                         aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                           <  2:779403/139‑1 (MQ=255)                                          ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                          <  1:1451650/139‑1 (MQ=255)                                           tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                         <  2:554167/139‑1 (MQ=255)                                                    ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                       >  2:858118/1‑100 (MQ=255)                                                    ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                       <  1:858118/100‑1 (MQ=255)                                                     tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGccccca                                                                                                                   >  1:1351695/1‑86 (MQ=255)                                                     tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGccccca                                                                                                                   <  2:1351695/87‑2 (MQ=255)                                                         tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                           <  2:1404586/139‑1 (MQ=255)                                                            tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                       >  1:442486/1‑124 (MQ=255)                                                            tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                       <  2:442486/124‑1 (MQ=255)                                                                            agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                 >  1:605397/1‑114 (MQ=255)                                                                            agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                 <  2:605397/114‑1 (MQ=255)                                                                                 tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                              >  1:230500/1‑80 (MQ=255)                                                                                 tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                              <  2:230500/80‑1 (MQ=255)                                                                                 tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                             <  1:30110/113‑1 (MQ=255)                                                                                 tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                             >  2:30110/1‑113 (MQ=255)                                                                                  gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                       <  1:467609/70‑1 (MQ=255)                                                                                  gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                       >  2:467609/1‑70 (MQ=255)                                                                                               gCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                     <  1:1375863/139‑1 (MQ=255)                                                                                               gCTCCCATGGCGAAAGGGAATGCGACATAATCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                     <  2:269694/59‑1 (MQ=38)                                                                                               gCTCCCATGGCGAAAGGGAATGCGACATAATCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                     >  1:269694/1‑59 (MQ=37)                                                                                                    cATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                >  1:1072627/1‑139 (MQ=255)                                                                                                           aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga         <  2:1072627/139‑1 (MQ=255)                                                                                                                  aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTcc                                                                                                   >  2:87905/1‑42 (MQ=255)                                                                                                                  aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTcc                                                                                                   <  1:87905/42‑1 (MQ=255)                                                                                                                  aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                 <  2:991819/92‑1 (MQ=255)                                                                                                                  aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                 >  1:991819/1‑92 (MQ=255)                                                                                                                  aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGcc  >  2:6497/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                 AGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC  >  NC_000913/3780894‑3781145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑