Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,688	G→A	*158* (TAG→TAA)	trmL →	tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,688	0	G	A	100.0%	261.8 / NA	80	*158* (TAG→TAA)	trmL	tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/45);  total (35/45)

CTGCCCGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCT  >  NC_000913/3781554‑3781822                                                                                                                                       |                                                                                                                                       ctgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTGTTGAGAGATTAATTAc                                                                                                                                    >  1:132890/1‑139 (MQ=255) ctgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTAc                                                                                                                                    >  1:1272738/1‑139 (MQ=255) ctgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTAc                                                                                                                                    >  2:1404489/1‑139 (MQ=255)        cTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGc                                                                                                                             >  1:1434420/1‑139 (MQ=255)          gAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCg                                                                                                                           <  2:1272738/139‑1 (MQ=255)           aaCAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCgg                                                                                                                          >  1:220577/1‑139 (MQ=255)            aCAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGa                                                                                                                         <  2:964508/139‑1 (MQ=255)            aCAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGa                                                                                                                         <  2:923256/139‑1 (MQ=255)              aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTGTTGAGAGATTAATTACTGTAGGCCGGATa                                                                                                                       <  2:132890/139‑1 (MQ=255)              aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              <  2:786270/132‑1 (MQ=255)              aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              >  1:786270/1‑132 (MQ=255)              aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATa                                                                                                                       <  2:407297/139‑1 (MQ=255)              aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATa                                                                                                                       <  2:1395564/139‑1 (MQ=255)                     tCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGa                                                                                                                         <  2:88553/130‑1 (MQ=255)                     tCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGa                                                                                                                         >  1:88553/1‑130 (MQ=255)                     tCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGAtt                                                                                                                >  1:499883/1‑139 (MQ=255)                          ttCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATc                                                                                                           <  2:610801/139‑1 (MQ=255)                                   tGCCGGACAGCAGCAGCATGAATCTGTCCAATGCAGTATCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGAGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGc                                                                                                  <  2:1288325/139‑1 (MQ=255)                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              >  2:627097/1‑109 (MQ=255)                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              <  1:627097/109‑1 (MQ=255)                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                <  2:499883/139‑1 (MQ=255)                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                >  2:866665/1‑139 (MQ=255)                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                <  1:513504/139‑1 (MQ=255)                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                >  2:1310976/1‑139 (MQ=255)                                        gACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGAt                                                                                             <  1:520847/139‑1 (MQ=255)                                          cAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           <  1:866665/139‑1 (MQ=255)                                            gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  2:1389988/139‑1 (MQ=255)                                            gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         >  1:1389988/1‑139 (MQ=255)                                            gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  2:1434420/139‑1 (MQ=255)                                            gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  2:1495704/139‑1 (MQ=255)                                             ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAg                                                                                        <  2:289323/139‑1 (MQ=255)                                              cGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAgg                                                                                       <  1:418747/139‑1 (MQ=255)                                                  gcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTTTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACAt                                                                                   <  1:1327/139‑1 (MQ=255)                                                     tGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           <  1:1213028/128‑1 (MQ=255)                                                     tGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           >  2:1213028/1‑128 (MQ=255)                                                       aaTCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGat                                                                              >  1:580900/1‑139 (MQ=255)                                                             tCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGatcccatc                                                                        >  2:501096/1‑139 (MQ=255)                                                                  tGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAg                                                                                        >  2:624402/1‑118 (MQ=255)                                                                  tGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAg                                                                                        <  1:624402/118‑1 (MQ=255)                                                                  tGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCAt                                                                   <  1:1310976/139‑1 (MQ=255)                                                                       tgtCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                <  2:1357911/137‑1 (MQ=255)                                                                       tgtCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                >  1:1357911/1‑137 (MQ=255)                                                                       tgtCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCa                                                              <  2:220577/139‑1 (MQ=255)                                                                        gtCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCaa                                                             <  2:423362/139‑1 (MQ=255)                                                                            gTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:580900/139‑1 (MQ=255)                                                                            gTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:213327/139‑1 (MQ=255)                                                                               gtggtgTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAga                                                                                                                     >  2:1296964/1‑76 (MQ=255)                                                                               gtggtgTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAga                                                                                                                     <  1:1296964/76‑1 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGt                                                                                                                                 <  1:893062/51‑1 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGt                                                                                                                                 >  2:893062/1‑51 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATc                                                                                                           <  2:114554/73‑1 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATc                                                                                                           >  1:114554/1‑73 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         >  1:100272/1‑123 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:100272/123‑1 (MQ=255)                                                                                            ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTg                                         <  1:1404489/139‑1 (MQ=255)                                                                                             cTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGa                                        >  1:480964/1‑139 (MQ=255)                                                                                             cTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGa                                        >  1:783001/1‑139 (MQ=255)                                                                                               ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                >  2:834653/1‑113 (MQ=255)                                                                                               ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                <  1:834653/113‑1 (MQ=255)                                                                                               ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTAAATACCGTTGaaa                                      >  1:1064277/1‑139 (MQ=255)                                                                                                 cggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATg                                    >  1:259959/1‑139 (MQ=255)                                                                                                      gTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATa                                               >  2:828069/1‑123 (MQ=255)                                                                                                      gTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATa                                               <  1:828069/123‑1 (MQ=255)                                                                                                            gTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCatat                         <  2:259959/139‑1 (MQ=255)                                                                                                                 cGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCAtt                    >  1:680015/1‑139 (MQ=255)                                                                                                                    gAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAg                                                                                <  2:480871/76‑1 (MQ=255)                                                                                                                    gAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAg                                                                                >  1:480871/1‑76 (MQ=255)                                                                                                                         gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  1:1210563/62‑1 (MQ=255)                                                                                                                         gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         >  2:1210563/1‑62 (MQ=255)                                                                                                                         gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         >  1:358341/1‑94 (MQ=255)                                                                                                                         gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:358341/94‑1 (MQ=255)                                                                                                                         gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCtt            >  2:66094/1‑139 (MQ=255)                                                                                                                           aTTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTAt          <  1:66094/139‑1 (MQ=255)                                                                                                                           aTTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTAt          <  1:501096/139‑1 (MQ=255)                                                                                                                            ttGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATc         <  2:680015/139‑1 (MQ=255)                                                                                                                                  gaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         >  1:950241/1‑53 (MQ=255)                                                                                                                                  gaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  2:950241/53‑1 (MQ=255)                                                                                                                                  gaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTgg                                >  2:1268216/1‑110 (MQ=255)                                                                                                                                  gaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTgg                                <  1:1268216/110‑1 (MQ=255)                                                                                                                                   attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                <  2:60811/45‑1 (MQ=255)                                                                                                                                   attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                >  1:60811/1‑45 (MQ=255)                                                                                                                                   attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCt  <  2:480964/139‑1 (MQ=255)                                                                                                                                    ttAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                >  2:789632/1‑76 (MQ=255)                                                                                                                                       |                                                                                                                                       CTGCCCGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCT  >  NC_000913/3781554‑3781822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑