Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,991	G→A	*272* (TAG→TAA)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,991	0	G	A	100.0%	321.4 / NA	99	*272* (TAG→TAA)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/50);  total (49/50)

ATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTG  >  NC_000913/344856‑345120                                                                                                                                        |                                                                                                                                  aTTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTc                                                                                                                                >  2:17542/1‑139 (MQ=255)           gTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                      <  2:936237/139‑1 (MQ=255)               tGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCAc                                                                                                                  >  2:815513/1‑139 (MQ=255)                    gTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                             <  2:47910/139‑1 (MQ=255)                    gTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                             <  2:577504/139‑1 (MQ=255)                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                             <  2:753149/137‑1 (MQ=255)                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                             >  1:753149/1‑137 (MQ=255)                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAg                                                                                                           >  2:1161518/1‑139 (MQ=255)                          aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                       <  2:670533/139‑1 (MQ=255)                            aTACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                     <  2:253242/139‑1 (MQ=255)                                      gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                           >  1:1217816/1‑139 (MQ=255)                                      gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                           <  2:574537/139‑1 (MQ=255)                                        tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                         >  1:756742/1‑139 (MQ=255)                                        tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                         <  1:815513/139‑1 (MQ=255)                                           gcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTACAAGTTGTTATTCCACCTGAGAGTg                                                                                      <  2:368169/139‑1 (MQ=255)                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCt                                                                                                                >  1:1396690/1‑112 (MQ=255)                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCt                                                                                                                <  2:1396690/112‑1 (MQ=255)                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                             >  2:323771/1‑115 (MQ=255)                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                             <  1:323771/115‑1 (MQ=255)                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                                                                                     >  1:1274666/1‑139 (MQ=255)                                              aTTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                   >  1:1397072/1‑139 (MQ=255)                                                tAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                 <  2:1274666/139‑1 (MQ=255)                                                 aaaTGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                  >  2:1004363/1‑121 (MQ=255)                                                 aaaTGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                  <  1:1004363/121‑1 (MQ=255)                                                   atgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                              <  1:359560/139‑1 (MQ=255)                                                         ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTc                                                                                                                                >  1:843013/1‑83 (MQ=255)                                                         ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTc                                                                                                                                <  2:843013/83‑1 (MQ=255)                                                         ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAgg                                                                        >  1:290707/1‑139 (MQ=255)                                                                tAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                 <  2:290707/139‑1 (MQ=255)                                                                   tAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              <  2:1199935/139‑1 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            <  2:1145932/139‑1 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            <  2:1217816/139‑1 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            <  1:17542/139‑1 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTg                                                                                                               <  1:900063/87‑1 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTg                                                                                                               >  2:900063/1‑87 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                 >  1:1262746/1‑133 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                 <  2:1262746/133‑1 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              >  1:621823/1‑136 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              <  2:621823/136‑1 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatc                                                           <  2:246208/139‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                            <  2:805845/72‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                            >  1:805845/1‑72 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                      >  1:1334561/1‑110 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                      <  2:1334561/110‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                         >  2:810919/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                         >  2:1589086/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                         >  2:987117/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                         >  2:1040456/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                         >  1:1106739/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                         >  1:569334/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            >  1:710869/1‑136 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            <  2:710869/136‑1 (MQ=255)                                                                         gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                   >  1:1250517/1‑96 (MQ=255)                                                                         gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                   <  2:1250517/96‑1 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                   >  1:474082/1‑95 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                   <  2:474082/95‑1 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                                                                                              >  2:1145793/1‑100 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                                                                                              <  1:1145793/100‑1 (MQ=255)                                                                           aGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                      <  1:216556/139‑1 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                             <  2:571550/61‑1 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                             >  1:571550/1‑61 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatatctgta                                               >  1:74893/1‑139 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatatctgta                                               <  1:1589086/139‑1 (MQ=255)                                                                                    gTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATg                                             <  1:1040456/139‑1 (MQ=255)                                                                                        cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                                               <  2:161100/101‑1 (MQ=255)                                                                                        cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                                               >  1:161100/1‑101 (MQ=255)                                                                                        cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCaa                                         <  2:1106739/139‑1 (MQ=255)                                                                                        cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCaa                                         <  2:569334/139‑1 (MQ=255)                                                                                           atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                            >  2:381686/1‑53 (MQ=255)                                                                                           atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                            <  1:381686/53‑1 (MQ=255)                                                                                           atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                       >  1:1232156/1‑74 (MQ=255)                                                                                           atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                       <  2:1232156/74‑1 (MQ=255)                                                                                             attatCCTGTAAATAAGCACGTTAAAATCCACTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAAt                                                                             <  2:821555/98‑1 (MQ=255)                                                                                             attatCCTGTAAATAAGCACGTTAAAATCCACTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAAt                                                                             >  1:821555/1‑98 (MQ=255)                                                                                                    tGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGc                                    >  2:598952/1‑132 (MQ=255)                                                                                                    tGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGc                                    <  1:598952/132‑1 (MQ=255)                                                                                                     gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            >  1:890672/1‑107 (MQ=255)                                                                                                     gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                            <  2:890672/107‑1 (MQ=255)                                                                                                     gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtc                            >  1:900818/1‑139 (MQ=255)                                                                                                     gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtc                            >  2:1307228/1‑139 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                         <  1:437840/61‑1 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                         >  2:437840/1‑61 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                           >  2:1438830/1‑139 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAATGGCATTCCAtct                           >  2:72014/1‑139 (MQ=255)                                                                                                        aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                 >  2:218223/1‑99 (MQ=255)                                                                                                        aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                 <  1:218223/99‑1 (MQ=255)                                                                                                        aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                       >  1:1491624/1‑109 (MQ=255)                                                                                                        aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                       <  2:1491624/109‑1 (MQ=255)                                                                                                           aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                      >  2:697149/1‑43 (MQ=255)                                                                                                           aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                      <  1:697149/43‑1 (MQ=255)                                                                                                             gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttttt                    >  1:798355/1‑139 (MQ=255)                                                                                                              cACGTTTAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              >  2:787093/1‑96 (MQ=255)                                                                                                              cACGTTTAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              <  1:787093/96‑1 (MQ=255)                                                                                                                cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGc                 >  2:498303/1‑139 (MQ=255)                                                                                                                               cacTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTtgtg  <  2:798355/139‑1 (MQ=255)                                                                                                                                  tctTTAATTCAGAAAGGGTTACACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              >  1:1369414/1‑76 (MQ=255)                                                                                                                                  tctTTAATTCAGAAAGGGTTACACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                              <  2:1369414/76‑1 (MQ=255)                                                                                                                                    tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                           <  1:481430/93‑1 (MQ=255)                                                                                                                                    tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                           >  2:481430/1‑93 (MQ=255)                                                                                                                                        |                                                                                                                                  ATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTG  >  NC_000913/344856‑345120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑