Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,005,694	G→A	R278H (CGT→CAT)	pldA →	outer membrane phospholipase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,005,694	0	G	A	100.0%	183.6 / NA	57	R278H (CGT→CAT)	pldA	outer membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/28);  total (29/28)

AGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGGCGCTGAAAAT  >  NC_000913/4005559‑4005828                                                                                                                                        |                                                                                                                                       aGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCatgt                                                                                                                                     <  1:1111475/139‑1 (MQ=255)  gTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTc                                                                                                                                    >  2:661921/1‑139 (MQ=255)   tACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCg                                                                                                                                   <  1:1137453/139‑1 (MQ=255)   tACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCg                                                                                                                                   >  2:1137453/1‑139 (MQ=255)        cTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGAtgtg                                                                                                                              <  2:464654/139‑1 (MQ=255)               acCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTa                                                                                                                       <  2:649377/139‑1 (MQ=255)                 cGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATg                                                                                                                     >  2:328934/1‑139 (MQ=255)                          cggcgCGGAGTTAGGCTTAAATGACTCGATCACCAAACATGAGCGTTTTTATACTAAGGTGTACAGCGGTTATGGCGAAGCGCTCATTGACTATAACTTCAACCAGACCTATGTCGGTGTGGGGGTTATACTAAACGAt                                                                                                            <  1:911091/139‑1 (MQ=255)                                    ttAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTtgat                                                                                                  <  2:252097/139‑1 (MQ=255)                                       ggCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               <  1:1347006/139‑1 (MQ=255)                                            aaGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGtt                                                                                          >  2:695544/1‑139 (MQ=255)                                             aGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTg                                                                                         >  1:905769/1‑139 (MQ=255)                                              gTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGa                                                                                        >  2:111055/1‑139 (MQ=255)                                                 aCCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTg                                                                                     >  2:1498138/1‑139 (MQ=255)                                                  cccGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCatgt                                                                                                                                     <  2:1531550/90‑1 (MQ=255)                                                  cccGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCatgt                                                                                                                                     >  1:1531550/1‑90 (MQ=255)                                                        cACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCa                                                                              >  1:1475883/1‑139 (MQ=255)                                                                aTGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGa                                                                      <  2:1475883/139‑1 (MQ=255)                                                                   tgCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATc                                                                   >  1:859492/1‑139 (MQ=255)                                                                    gcgcCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATcc                                                                  <  1:328934/139‑1 (MQ=255)                                                                     cgcCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCa                                                                 <  1:695544/139‑1 (MQ=255)                                                                       ccTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGc                                                               >  1:1521038/1‑139 (MQ=255)                                                                            ataCTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCa                                                          <  1:233312/139‑1 (MQ=255)                                                                                  aGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTg                                                                                     >  2:67572/1‑106 (MQ=255)                                                                                  aGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTg                                                                                     <  1:67572/106‑1 (MQ=255)                                                                                  aGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAgg                                                        >  1:389556/1‑135 (MQ=255)                                                                                  aGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAgg                                                        <  2:389556/135‑1 (MQ=255)                                                                                    gTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               <  1:203724/94‑1 (MQ=255)                                                                                    gTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               >  2:203724/1‑94 (MQ=255)                                                                                    gTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTAcc                                                  <  1:661921/139‑1 (MQ=255)                                                                                    gTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTAcc                                                  >  1:1148512/1‑139 (MQ=255)                                                                                     tttACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCaa                                                                             <  2:410298/111‑1 (MQ=255)                                                                                     tttACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCaa                                                                             >  1:410298/1‑111 (MQ=255)                                                                                              gCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGtt                                                                                  <  1:64267/97‑1 (MQ=255)                                                                                              gCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGtt                                                                                  >  2:64267/1‑97 (MQ=255)                                                                                              gCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAgaga                                        >  2:1125494/1‑139 (MQ=255)                                                                                                tATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACt                                      <  2:766109/139‑1 (MQ=255)                                                                                                       aaTCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCa                               <  2:1148512/139‑1 (MQ=255)                                                                                                        aTCGCTCATCGACTATAACTTCAACCAGACCCATGTc                                                                                                                                    <  2:886127/37‑1 (MQ=255)                                                                                                        aTCGCTCATCGACTATAACTTCAACCAGACCCATGTc                                                                                                                                    >  1:886127/1‑37 (MQ=38)                                                                                                        aTCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCAt                              >  2:89182/1‑139 (MQ=255)                                                                                                        aTCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCAt                              >  2:1506910/1‑139 (MQ=255)                                                                                                         tCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               <  2:81774/73‑1 (MQ=255)                                                                                                         tCGCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               >  1:81774/1‑73 (MQ=255)                                                                                                           gCTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGc                           <  1:111055/139‑1 (MQ=255)                                                                                                            cTCATCGACTATAACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCa                          <  2:476527/139‑1 (MQ=255)                                                                                                                       taACTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGA‑TTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCa              >  2:540550/1‑139 (MQ=255)                                                                                                                        aaCTTCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGA‑TTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAg             <  1:540550/139‑1 (MQ=255)                                                                                                                           ttCAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGgc           <  1:1506910/139‑1 (MQ=255)                                                                                                                             cAACCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGgcgc         >  2:1461391/1‑139 (MQ=255)                                                                                                                              aaCCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGGCGCt        <  1:1461391/139‑1 (MQ=255)                                                                                                                               aCCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               <  1:856884/51‑1 (MQ=255)                                                                                                                               aCCAGACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGaa                                                                                               >  2:856884/1‑51 (MQ=255)                                                                                                                                   gACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAAt                                                                           >  1:1548625/1‑67 (MQ=255)                                                                                                                                   gACCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAAt                                                                           <  2:1548625/67‑1 (MQ=255)                                                                                                                                    aCCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAg                                                                                       <  1:71174/54‑1 (MQ=255)                                                                                                                                    aCCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAg                                                                                       >  2:71174/1‑54 (MQ=255)                                                                                                                                    aCCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGGCGCTGAAAAt  >  2:529158/1‑139 (MQ=255)                                                                                                                                    aCCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGGCGCTGAAAAt  >  1:1282282/1‑139 (MQ=255)                                                                                                                                    aCCCATGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGGCGCTGAAAAt  >  2:1580188/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AGTACAACTGGAACACCGGCTACGGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAGGTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGTGTGGGGGTTATGCTAAACGATTTGTTTTGATGAACGGTTGAGTGGTTGGCAAATCTGGAATCCAGCATCCAGGATTACCCTCTCAGAGACTAAAAGCATTGCAGTTTCTCGCGCAGGCGCTGAAAAT  >  NC_000913/4005559‑4005828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑