Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,118,583	T→C	T23T (ACT→ACC)	zapB →	FtsZ stabilizer, septal ring assembly factor, cell division stimulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,118,583	0	T	C	100.0%	275.2 / NA	76	T23T (ACT→ACC)	zapB	FtsZ stabilizer, septal ring assembly factor, cell division stimulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/34);  total (42/34)

GCGTCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAG  >  NC_000913/4118454‑4118718                                                                                                                                  |                                                                                                                                        gCGTCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAg                                                                                                                                <  2:678774/139‑1 (MQ=255)   gTCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGAt                                                                                                                              <  1:890295/139‑1 (MQ=255)   gTCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGAt                                                                                                                              <  2:1368062/139‑1 (MQ=255)    tCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATg                                                                                                                             >  2:1118347/1‑139 (MQ=255)    tCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATg                                                                                                                             >  2:1341394/1‑139 (MQ=255)    tCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATg                                                                                                                             >  1:400582/1‑139 (MQ=255)     cACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATgg                                                                                                                            <  2:400582/139‑1 (MQ=255)      aCCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGa                                                                                                                           >  2:516420/1‑139 (MQ=255)             aTCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGaa                                                                                                                    >  2:1363818/1‑139 (MQ=255)             aTCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGaa                                                                                                                    <  1:356868/139‑1 (MQ=255)                 ggACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAACTCGAAGAGc                                                                                                                >  1:948790/1‑139 (MQ=255)                  gACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGc                                                                                                                                  >  2:391096/1‑120 (MQ=255)                  gACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGc                                                                                                                                  <  1:391096/120‑1 (MQ=255)                  gACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCt                                                                                                               >  1:134737/1‑139 (MQ=255)                       ggATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTgaaag                                                                                                          >  2:445462/1‑139 (MQ=255)                        gATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTgaaaga                                                                                                         >  1:1117116/1‑139 (MQ=255)                                   aTCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACt                                                                                              <  2:1412864/139‑1 (MQ=255)                                    tCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTc                                                                                             >  1:1193792/1‑139 (MQ=255)                                     cAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            >  1:126379/1‑139 (MQ=255)                                       aCGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACt                                                                                          >  1:1180131/1‑139 (MQ=255)                                            ttGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGc                                                                                     <  1:1341394/139‑1 (MQ=255)                                            ttGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGc                                                                                     <  1:445462/139‑1 (MQ=255)                                             tGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCa                                                                                    <  1:27240/139‑1 (MQ=255)                                                 aTTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGaa                                                                                <  1:1118347/139‑1 (MQ=255)                                                  ttCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAg                                                                               >  2:995090/1‑139 (MQ=255)                                                          agGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAAt                                                                       >  1:407017/1‑139 (MQ=255)                                                          agGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAAt                                                                       >  2:1169791/1‑139 (MQ=255)                                                            gTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTTACTTTCGCAGGAAGTTCAAAATGc                                                                     >  1:1574147/1‑139 (MQ=255)                                                                     tGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCa                                                            >  1:35891/1‑139 (MQ=255)                                                                     tGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCa                                                            >  2:53407/1‑139 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTga                                                                                                             <  2:486811/89‑1 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTga                                                                                                             >  1:486811/1‑89 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGCAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAg                                                           >  2:1021353/1‑139 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            >  1:1093573/1‑106 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            <  2:1093573/106‑1 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            >  2:214960/1‑106 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            <  1:214960/106‑1 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAg                                                           >  2:907538/1‑139 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAg                                                           >  1:1124711/1‑139 (MQ=255)                                                                      gTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAg                                                           <  2:1117116/139‑1 (MQ=255)                                                                       tCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGaaaaaaa                                                                                                   <  2:324489/98‑1 (MQ=255)                                                                       tCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGaaaaaaa                                                                                                   >  1:324489/1‑98 (MQ=255)                                                                       tCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAgc                                                          >  2:1018621/1‑139 (MQ=255)                                                                       tCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAgc                                                          >  1:1401360/1‑139 (MQ=255)                                                                          ttAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGcgcg                                                       <  1:907538/139‑1 (MQ=255)                                                                          ttAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGcgcg                                                       <  2:407017/139‑1 (MQ=255)                                                                          ttAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGcgcg                                                       <  2:126379/139‑1 (MQ=255)                                                                          ttAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGcgcg                                                       <  2:1124711/139‑1 (MQ=255)                                                                            aGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGaa                                                     >  1:106926/1‑139 (MQ=255)                                                                            aGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGaa                                                     <  1:516420/139‑1 (MQ=255)                                                                                gtgtTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGc                                                 <  1:1021353/139‑1 (MQ=255)                                                                                 tgtTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCt                                                >  1:411960/1‑139 (MQ=255)                                                                                 tgtTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCt                                                >  2:1502345/1‑139 (MQ=255)                                                                                  ggttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGGTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCCGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTg                                               >  2:519534/3‑139 (MQ=255)                                                                                   tttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            >  1:78671/1‑93 (MQ=255)                                                                                   tttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCa                                                                                            <  2:78671/93‑1 (MQ=255)                                                                                   tttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCACGAAGAGCTgg                                              >  1:1291670/1‑139 (MQ=255)                                                                                      gagaAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc                                           <  2:411960/139‑1 (MQ=255)                                                                                      gagaAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc                                           <  2:948790/139‑1 (MQ=255)                                                                                      gagaAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc                                           <  2:134737/139‑1 (MQ=255)                                                                                           aCTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGa                                                                                                                           >  1:726049/1‑54 (MQ=255)                                                                                           aCTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGa                                                                                                                           <  2:726049/54‑1 (MQ=255)                                                                                           aCTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc                                           <  1:780318/134‑1 (MQ=255)                                                                                           aCTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc                                           >  2:780318/1‑134 (MQ=255)                                                                                             tGGAAGCATAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGAt                                                                                                                              <  2:979620/49‑1 (MQ=255)                                                                                             tGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGAt                                                                                                                              >  1:979620/1‑49 (MQ=255)                                                                                               gAAGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaaca                                  <  2:1180131/139‑1 (MQ=255)                                                                                                 aGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaacaac                                <  2:35891/139‑1 (MQ=255)                                                                                                 aGCAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaacaac                                >  2:70543/1‑139 (MQ=255)                                                                                                   cAAAAGTACAGCAGGCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCa                              <  1:53407/139‑1 (MQ=255)                                                                                                                ggCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCg                                          >  1:1607372/1‑114 (MQ=255)                                                                                                                ggCGATTGATACCATCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCg                                          <  2:1607372/114‑1 (MQ=255)                                                                                                                             aTCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaacaa                                 >  2:1568305/1‑110 (MQ=255)                                                                                                                             aTCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaacaa                                 <  1:1568305/110‑1 (MQ=255)                                                                                                                             aTCACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTCGc    >  1:1071204/1‑139 (MQ=255)                                                                                                                               cACCCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGATAGAACAGCAGAACGGCTGGCAg  >  2:29928/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        GCGTCACCGGTAATCGGGACGAGGATTTTTATCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAAAACAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAG  >  NC_000913/4118454‑4118718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑