Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,158,581	C→T	T31M (ACG→ATG)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,158,581	0	C	T	100.0%	303.1 / NA	92	T31M (ACG→ATG)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/42);  total (50/42)

TCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAA  >  NC_000913/4158455‑4158716                                                                                                                               |                                                                                                                                        tCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCTAGCCGATGCTTAGCGGGCa                                                                                                                             <  1:1604520/139‑1 (MQ=255)  cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCaa                                                                                                                            >  2:1247464/1‑139 (MQ=255)  cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCaa                                                                                                                            >  2:1102863/1‑139 (MQ=255)  cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCaa                                                                                                                            >  1:1359552/1‑139 (MQ=255)  cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCaa                                                                                                                            >  1:46999/1‑139 (MQ=255)       tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCg                                                                                                                       >  2:1510464/1‑139 (MQ=255)       tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCg                                                                                                                       >  2:1130020/1‑139 (MQ=255)            tATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGc                                                                                                                  <  1:1522704/139‑1 (MQ=255)             aTCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCt                                                                                                                 <  2:306964/139‑1 (MQ=255)              tCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTg                                                                                                                >  1:1367404/1‑139 (MQ=255)              tCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTg                                                                                                                >  2:864376/1‑139 (MQ=255)               cGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTgg                                                                                                               >  2:62460/1‑139 (MQ=255)                 tGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGt                                                                                                                      >  2:1577796/1‑130 (MQ=255)                 tGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGt                                                                                                                      <  1:1577796/130‑1 (MQ=255)                   gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGt                                                                                                                      <  2:1531902/128‑1 (MQ=255)                   gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGt                                                                                                                      >  1:1531902/1‑128 (MQ=255)                   gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTa                                                                                                                     >  2:1053680/1‑129 (MQ=255)                   gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTa                                                                                                                     <  1:1053680/129‑1 (MQ=255)                          aggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTTGTAAGCTGGAAGATGAGCTg                                                                                                    >  1:160190/1‑139 (MQ=255)                             atggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                 <  1:1287256/139‑1 (MQ=255)                                 atAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                                             <  1:1247464/139‑1 (MQ=255)                                         tatTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTg                                                                                     >  1:617846/1‑139 (MQ=255)                                          atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAg                                                                                                                   >  1:1443661/1‑108 (MQ=255)                                          atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAg                                                                                                                   <  2:1443661/108‑1 (MQ=255)                                           ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGa                                                                                   >  1:450490/1‑139 (MQ=255)                                            tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                  <  2:1433169/139‑1 (MQ=255)                                            tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                  <  1:1510464/139‑1 (MQ=255)                                            tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCAGTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                  >  2:1051555/1‑139 (MQ=255)                                                gATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGc                                                                                       <  1:225720/130‑1 (MQ=255)                                                gATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGc                                                                                       >  2:225720/1‑130 (MQ=255)                                                 aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             <  2:1367404/139‑1 (MQ=255)                                                 aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             >  2:63491/1‑139 (MQ=255)                                                 aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             >  2:1414647/1‑139 (MQ=255)                                                   cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGGTGGAGCGGACCa                                                                           >  1:944392/1‑139 (MQ=255)                                                    ttGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                                          <  1:969845/139‑1 (MQ=255)                                                     tGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             >  2:562637/1‑135 (MQ=255)                                                     tGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             <  1:562637/135‑1 (MQ=255)                                                       aGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCg                                                                       >  1:657791/1‑139 (MQ=255)                                                       aGTACCTGGTGGCATTGGCTGAACAC‑‑CCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                                                                     <  1:329772/139‑1 (MQ=255)                                                         tACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                                                                     <  1:1102863/139‑1 (MQ=255)                                                         tACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                                                                     <  2:617846/139‑1 (MQ=255)                                                           ccTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTaaa                                                                   >  1:861092/1‑139 (MQ=255)                                                            cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCg                                                                       <  1:1220143/134‑1 (MQ=255)                                                            cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCg                                                                       >  2:1220143/1‑134 (MQ=255)                                                            cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAg                                                                  >  1:434782/1‑139 (MQ=255)                                                             tggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                 >  1:1198316/1‑139 (MQ=255)                                                              ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTa                                                                                                                                    <  1:1445207/71‑1 (MQ=255)                                                              ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTa                                                                                                                                    >  2:1445207/1‑71 (MQ=255)                                                              ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                >  2:408403/1‑139 (MQ=255)                                                              ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                >  2:247443/1‑139 (MQ=255)                                                              ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                >  2:701248/1‑139 (MQ=255)                                                                tggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgtt                                                              >  1:70715/1‑139 (MQ=255)                                                                 ggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttg                                                             >  1:1013592/1‑139 (MQ=255)                                                                  gCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                 >  1:1578266/1‑134 (MQ=255)                                                                  gCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                 <  2:1578266/134‑1 (MQ=255)                                                                   cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                >  1:1365650/1‑134 (MQ=255)                                                                   cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                <  2:1365650/134‑1 (MQ=255)                                                                     ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCa                                                         >  2:829376/1‑139 (MQ=255)                                                                     ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCa                                                         >  2:1276324/1‑139 (MQ=255)                                                                      tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                 <  2:1154083/98‑1 (MQ=255)                                                                      tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                 >  1:1154083/1‑98 (MQ=255)                                                                      tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                                        >  1:558443/1‑139 (MQ=255)                                                                        gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttgt                                                            <  2:706656/133‑1 (MQ=255)                                                                        gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttgt                                                            >  1:706656/1‑133 (MQ=255)                                                                        gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAccc                                                      <  2:434782/139‑1 (MQ=255)                                                                         cTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCa                                                     <  1:62460/139‑1 (MQ=255)                                                                            aaCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGc                                                  >  2:578728/1‑139 (MQ=255)                                                                                 cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                 <  1:622803/87‑1 (MQ=255)                                                                                 cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                 >  2:622803/1‑87 (MQ=255)                                                                                 cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGaa                                             >  1:490975/1‑139 (MQ=255)                                                                                 cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGaa                                             <  1:829376/139‑1 (MQ=255)                                                                                 cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGaa                                             >  1:1416966/1‑139 (MQ=255)                                                                                 cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGaa                                             >  2:49103/1‑139 (MQ=255)                                                                                   ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                           >  2:80193/1‑139 (MQ=255)                                                                                   ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                           <  1:247443/139‑1 (MQ=255)                                                                                      ttttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgctg                                        <  1:1414647/139‑1 (MQ=255)                                                                                      ttttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgctg                                        <  2:46999/139‑1 (MQ=255)                                                                                          cGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCtgg                                    <  2:558443/139‑1 (MQ=255)                                                                                               tGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                               <  2:1013592/139‑1 (MQ=255)                                                                                               tGCGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                               <  2:1198316/139‑1 (MQ=255)                                                                                                 cGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGc                                                                                                                  <  1:864817/54‑1 (MQ=255)                                                                                                 cGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGc                                                                                                                  >  2:864817/1‑54 (MQ=255)                                                                                                 cGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                                      <  2:204428/66‑1 (MQ=255)                                                                                                 cGGCAGATTCCTGCCACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                                      >  1:204428/1‑66 (MQ=255)                                                                                                              ccACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTa                                                                                                                     >  2:1518899/1‑38 (MQ=38)                                                                                                              ccACGTTAGCCAGCCGATGCTTAGCGGGCAAATTCGTa                                                                                                                     <  1:1518899/38‑1 (MQ=38)                                                                                                                    tAGCCAGCCGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGt          <  2:490975/139‑1 (MQ=255)                                                                                                                     agccagccGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTg         <  2:1416966/139‑1 (MQ=255)                                                                                                                       ccagccGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAg       <  1:49103/139‑1 (MQ=255)                                                                                                                           ccGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             <  2:695339/65‑1 (MQ=255)                                                                                                                           ccGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                             >  1:695339/1‑65 (MQ=255)                                                                                                                            cGATGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa  <  2:657791/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        TCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAA  >  NC_000913/4158455‑4158716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑