Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,377,189 C→T H105H (CAC→CAT
*178* (TAG→TAA
sugE →
blc ←
multidrug efflux system protein
outer membrane lipoprotein cell division and growth lipocalin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,377,1890CT100.0% 173.1 / NA 54H105H (CAC→CAT
*178* (TAG→TAA
sugE
blc
multidrug efflux system protein
outer membrane lipoprotein cell division and growth lipocalin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

GTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTC  >  NC_000913/4377057‑4377317
                                                                                                                                    |                                                                                                                                
gtgGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGCGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCattaacta                                                                                                                            >  2:317627/1‑139 (MQ=255)
 tgGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTAc                                                                                                                           <  1:1391437/139‑1 (MQ=255)
    aCGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAg                                                                                                                        <  1:1270139/139‑1 (MQ=255)
     cGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                       <  1:13148/139‑1 (MQ=255)
         tATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgc                                                                                                                   >  2:1090956/1‑139 (MQ=255)
                 cAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCNAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCa                                                                                                           <  2:1343977/139‑1 (MQ=255)
                                 aaCCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCTTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGTCTGCTGTACCCAAATAAGTTTACTGACa                                                                                           >  1:706455/1‑139 (MQ=255)
                                       cATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAAc                                                                                     >  1:963266/1‑139 (MQ=255)
                                           gtgCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               <  2:698498/125‑1 (MQ=255)
                                           gtgCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               >  1:698498/1‑125 (MQ=255)
                                            tgctgcTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTc                                                                                >  2:1236417/1‑139 (MQ=255)
                                                 ctCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCggg                                                                           >  1:1557957/1‑139 (MQ=255)
                                                      tGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTcgcg                                                                      >  1:233746/1‑139 (MQ=255)
                                                          tCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTg                                                                  <  2:233746/139‑1 (MQ=255)
                                                           ccGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGc                                                                 >  1:1612816/1‑139 (MQ=255)
                                                                    cGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtctc                                                        <  2:485359/139‑1 (MQ=255)
                                                                      aTGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCt                                                      >  1:1089007/1‑139 (MQ=255)
                                                                           ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgc                                                                                                                   >  2:300781/1‑73 (MQ=255)
                                                                           ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgc                                                                                                                   <  1:300781/73‑1 (MQ=255)
                                                                           ccTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTc                                                 >  2:907437/1‑139 (MQ=255)
                                                                             tGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGTGACTGCCAGCATCTCCTGTTTCAc                                               <  1:1090956/139‑1 (MQ=255)
                                                                              ggCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACt                                              <  2:1557957/139‑1 (MQ=255)
                                                                              ggCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACt                                              <  2:963266/139‑1 (MQ=255)
                                                                                    tCTGGCGTTAGTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtc                                                          <  2:806055/121‑1 (MQ=255)
                                                                                    tCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACGCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtc                                                          >  1:806055/1‑121 (MQ=255)
                                                                                    tCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCa                                        >  1:987371/1‑139 (MQ=255)
                                                                                      tGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               <  2:468899/82‑1 (MQ=255)
                                                                                      tGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               >  1:468899/1‑82 (MQ=255)
                                                                                             aaTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTa                                                                                                               <  1:112788/59‑1 (MQ=255)
                                                                                             aaTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTa                                                                                                               >  2:112788/1‑59 (MQ=255)
                                                                                              aTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTg                              <  1:1236417/139‑1 (MQ=255)
                                                                                                  tATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGt                          >  1:328109/1‑139 (MQ=255)
                                                                                                   aTTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTg                         >  1:412256/1‑139 (MQ=255)
                                                                                                     tGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCg                       <  1:89184/139‑1 (MQ=255)
                                                                                                       gggATTATTGGTCTCAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTTACATCAAACCCTTCCCGGGTCTCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCggg                     <  2:987371/139‑1 (MQ=255)
                                                                                                        ggATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGga                    <  2:1318696/139‑1 (MQ=255)
                                                                                                         gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               >  2:1255016/1‑63 (MQ=255)
                                                                                                         gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               <  1:1255016/63‑1 (MQ=255)
                                                                                                         gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTcc                                                                               >  2:945428/1‑79 (MQ=255)
                                                                                                         gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgag                   >  1:1513627/1‑139 (MQ=255)
                                                                                                         gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATCTACTGACATCAAACCCTTcc                                                                               <  1:945428/79‑1 (MQ=255)
                                                                                                          attattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgaga                  <  2:328109/139‑1 (MQ=255)
                                                                                                           ttattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGagag                 <  2:1513627/139‑1 (MQ=255)
                                                                                                            tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGc                        <  2:705445/131‑1 (MQ=255)
                                                                                                            tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGc                        >  1:705445/1‑131 (MQ=255)
                                                                                                            tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGt                <  1:907437/139‑1 (MQ=255)
                                                                                                                 gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               <  2:367225/55‑1 (MQ=255)
                                                                                                                 gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                               >  1:367225/1‑55 (MQ=255)
                                                                                                                 gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGt                                                    >  2:545194/1‑98 (MQ=255)
                                                                                                                 gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGt                                                    <  1:545194/98‑1 (MQ=255)
                                                                                                                   cTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCcaca         >  1:928493/1‑139 (MQ=255)
                                                                                                                    tGAAACTCAGCTCTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtc                                                          <  2:992130/89‑1 (MQ=255)
                                                                                                                    tGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACGGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtc                                                          >  1:992130/1‑89 (MQ=255)
                                                                                                                      aaaCTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGt      >  2:1221464/1‑139 (MQ=255)
                                                                                                                          tCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAACTTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTc  <  2:706455/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
GTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTC  >  NC_000913/4377057‑4377317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: