Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,516 C→T *92* (TAG→TAA yjfY ← YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,5160CT100.0% 128.8 / NA 40*92* (TAG→TAAyjfYYhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

AAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGC  >  NC_000913/4424381‑4424650
                                                                                                                                       |                                                                                                                                      
aaGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                     <  2:858408/139‑1 (MQ=255)
  gTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCg                                                                                                                                   <  1:145953/139‑1 (MQ=255)
          cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAatat                                                                                                                           >  2:1204671/1‑139 (MQ=255)
           gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATc                                                                                                                          >  2:1215164/1‑139 (MQ=255)
            cgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATcc                                                                                                                         >  1:360877/1‑139 (MQ=255)
             gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCcg                                                                                                                        >  2:109107/1‑139 (MQ=255)
                           cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTgatat                                                                                                          >  1:1350583/1‑135 (MQ=255)
                                 cGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGt                                                                                                   <  1:734695/139‑1 (MQ=255)
                                    gagaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTAc                                                                                                <  1:1244993/139‑1 (MQ=255)
                                        tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTccc                                                                                            <  1:109107/139‑1 (MQ=255)
                                         aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTccc                                                                                            <  2:1555023/138‑1 (MQ=255)
                                         aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTccc                                                                                            >  1:1555023/1‑138 (MQ=255)
                                              gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTc                                                                                      >  1:33084/1‑139 (MQ=255)
                                               tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTcc                                                                                     <  2:978128/139‑1 (MQ=255)
                                                 tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCg                                                                                   >  1:623912/1‑139 (MQ=255)
                                                 tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCg                                                                                   >  2:798292/1‑139 (MQ=255)
                                                      ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                     <  1:944330/85‑1 (MQ=255)
                                                      ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                     >  2:944330/1‑85 (MQ=255)
                                                       cGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAg                                                                             <  1:798292/139‑1 (MQ=255)
                                                           gCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAAtt                                                                         <  1:492037/139‑1 (MQ=255)
                                                            ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                        <  2:33084/139‑1 (MQ=255)
                                                              gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                      <  1:1484607/139‑1 (MQ=255)
                                                                      aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgtagta                                                              >  2:1268848/1‑139 (MQ=255)
                                                                             gCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcg                                                       <  2:1350583/139‑1 (MQ=255)
                                                                                 ccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTcc                                                                                     <  1:841222/105‑1 (MQ=255)
                                                                                 ccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTcc                                                                                     >  2:841222/1‑105 (MQ=255)
                                                                                             gcaaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGttt                                       >  2:538794/1‑139 (MQ=255)
                                                                                                             cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTg                       >  1:1093077/1‑139 (MQ=255)
                                                                                                             cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTg                       <  2:360877/139‑1 (MQ=255)
                                                                                                                       ttGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                     >  2:1144870/1‑115 (MQ=255)
                                                                                                                       ttGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                     <  1:1144870/115‑1 (MQ=255)
                                                                                                                           ttATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCttt         <  2:1532523/139‑1 (MQ=255)
                                                                                                                           ttATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCttt         <  2:1093077/139‑1 (MQ=255)
                                                                                                                            tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                  <  2:46647/97‑1 (MQ=255)
                                                                                                                            tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                  >  1:46647/1‑97 (MQ=255)
                                                                                                                             ataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCa       >  2:242524/1‑139 (MQ=255)
                                                                                                                             ataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCa       >  2:467493/1‑139 (MQ=255)
                                                                                                                             ataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCa       <  1:1215164/139‑1 (MQ=255)
                                                                                                                                   ggTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTaa                             <  2:461640/111‑1 (MQ=255)
                                                                                                                                   ggTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTaa                             >  1:461640/1‑111 (MQ=255)
                                                                                                                                    gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCt                                           <  2:887584/96‑1 (MQ=255)
                                                                                                                                    gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGAc                     >  2:247261/1‑118 (MQ=255)
                                                                                                                                    gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc  <  2:552980/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGC  >  NC_000913/4424381‑4424650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: