Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,510,690 C→T *256* (TAG→TAA fecE ← ferric citrate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,510,6900CT98.8% 257.7 / NA 80*256* (TAG→TAAfecEferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/41);  minor base G (0/1);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCG  >  NC_000913/4510554‑4510819
                                                                                                                                        |                                                                                                                                 
gCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTa                                                                                                                                 <  2:1568722/139‑1 (MQ=255)
     aagcaagcGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCa                                                                                                                            >  2:1439042/1‑139 (MQ=255)
      agcaagcGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCAt                                                                                                                           >  2:507267/1‑139 (MQ=255)
          agcGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAgg                                                                                                                       <  1:507267/139‑1 (MQ=255)
                 ggACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCg                                                                                                                <  1:1439042/139‑1 (MQ=255)
                       tGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGc                                                                                                          >  1:706738/1‑139 (MQ=255)
                             ttGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    >  2:1536455/1‑139 (MQ=255)
                                gTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTCAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCg                                                                                                 >  1:984231/1‑139 (MQ=255)
                                 tAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGATGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCgg                                                                                                >  1:1413511/1‑139 (MQ=255)
                                  aaTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGcac                                                                                                                    >  2:476738/1‑118 (MQ=255)
                                  aaTCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGcac                                                                                                                    <  1:476738/118‑1 (MQ=255)
                                    tCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    >  1:1217418/1‑132 (MQ=255)
                                    tCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    <  2:1217418/132‑1 (MQ=255)
                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    <  2:22382/131‑1 (MQ=255)
                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    >  1:22382/1‑131 (MQ=255)
                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                            <  2:682369/139‑1 (MQ=255)
                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                            <  1:76314/139‑1 (MQ=255)
                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                            >  2:241774/1‑139 (MQ=255)
                                     cTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCg                                                                                            <  1:241774/139‑1 (MQ=255)
                                        tCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCgggg                                                                                         >  2:1592661/1‑139 (MQ=255)
                                        tCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACAGCCTGTGCAATTTACCTCATTAGGcac                                                                                                                    <  1:1578697/112‑1 (MQ=255)
                                        tCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACAGCCTGTGCAATTTACCTCATTAGGcac                                                                                                                    >  2:1578697/1‑112 (MQ=255)
                                               ccGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                              <  1:1445040/111‑1 (MQ=255)
                                               ccGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                              >  2:1445040/1‑111 (MQ=255)
                                                cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCgggg                                                                                         <  1:1171215/131‑1 (MQ=255)
                                                cGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCgggg                                                                                         >  2:1171215/1‑131 (MQ=255)
                                                       gTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTcc                                                                          <  1:1487234/139‑1 (MQ=255)
                                                        ttCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCa                                                                         >  2:704697/1‑139 (MQ=255)
                                                            gCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCt                                                                     >  2:419743/1‑139 (MQ=255)
                                                             ccccAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACAt                                                                                                                  <  1:783122/93‑1 (MQ=255)
                                                             ccccAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACAt                                                                                                                  >  2:783122/1‑93 (MQ=255)
                                                                 aGTCTCAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGAGTATTTCCGCTTCCACCCTGAATa                                                                <  2:984231/139‑1 (MQ=255)
                                                                  gTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATAc                                                               >  1:1292427/1‑139 (MQ=255)
                                                                       aaCAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                              >  1:823266/1‑87 (MQ=255)
                                                                       aaCAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                              <  2:823266/87‑1 (MQ=255)
                                                                        aCAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                              >  1:1448093/1‑102 (MQ=255)
                                                                        aCAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                              <  2:1448093/102‑1 (MQ=255)
                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      <  1:452980/139‑1 (MQ=255)
                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      <  2:706738/139‑1 (MQ=255)
                                                                           ggCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      <  2:1292427/139‑1 (MQ=255)
                                                                            gCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGc                                                     <  2:770574/139‑1 (MQ=255)
                                                                             cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                              <  2:30266/81‑1 (MQ=255)
                                                                             cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGc                                                                                                              >  1:30266/1‑81 (MQ=255)
                                                                             cTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCa                                                    <  1:1536455/139‑1 (MQ=255)
                                                                                  tGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCt                                               <  1:1017267/139‑1 (MQ=255)
                                                                                      gcCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGt                                          >  1:1322329/1‑139 (MQ=255)
                                                                                        cagtccagtTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGtca                                        <  2:1322329/139‑1 (MQ=255)
                                                                                                 ttcattcgGTCATGGTTTTATTCTTACGGCCTGTGTAATGTATCTCATTAGGCACATCGGCCCGCCAGATACCGGCGCGGGTTGTATTTCCGCTGCCACGCTGAATACTGTTCTCAGCAAGCCTGGGGTCATCACctct                                <  1:1149508/139‑1 (MQ=255)
                                                                                                    attcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                             <  2:1159045/59‑1 (MQ=255)
                                                                                                    attcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                             >  1:1159045/1‑59 (MQ=255)
                                                                                                    attcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCtcttct                             >  2:20469/1‑139 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                             >  2:1312505/1‑58 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGcc                                                                                                             <  1:1312505/58‑1 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                              <  2:96082/73‑1 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                              >  1:96082/1‑73 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGgt                                                                                        <  2:1114734/79‑1 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGgt                                                                                        >  1:1114734/1‑79 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATAc                                                               <  2:859252/104‑1 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATAc                                                               >  1:859252/1‑104 (MQ=255)
                                                                                                     ttcaGTCGTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTg                            >  1:747556/1‑139 (MQ=255)
                                                                                                            gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                              >  1:1016829/1‑66 (MQ=255)
                                                                                                            gTGGTTTGGTTCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCt                                                                                              <  2:1016829/66‑1 (MQ=255)
                                                                                                             tggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCt                    <  1:20469/139‑1 (MQ=255)
                                                                                                              ggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    >  2:87930/1‑58 (MQ=255)
                                                                                                              ggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATa                                                                                                    <  1:87930/58‑1 (MQ=255)
                                                                                                              ggtttggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCtt                   >  2:975236/1‑139 (MQ=255)
                                                                                                                   ggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      >  1:1600798/1‑99 (MQ=255)
                                                                                                                   ggttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      <  2:1600798/99‑1 (MQ=255)
                                                                                                                    gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCa             >  2:397971/1‑139 (MQ=255)
                                                                                                                    gttCTTACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCa             <  2:747556/139‑1 (MQ=255)
                                                                                                                         tACGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACa        >  1:772696/1‑139 (MQ=255)
                                                                                                                          aCGGCCTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACAt       <  1:419743/139‑1 (MQ=255)
                                                                                                                              ccTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      >  1:558724/1‑88 (MQ=255)
                                                                                                                              ccTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAg                                                      <  2:558724/88‑1 (MQ=255)
                                                                                                                               cTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCg  <  2:772696/139‑1 (MQ=255)
                                                                                                                               cTGTGCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCg  <  1:397971/139‑1 (MQ=255)
                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACctc                                 <  1:378783/105‑1 (MQ=255)
                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACctc                                 >  2:378783/1‑105 (MQ=255)
                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGcc                     >  1:1078387/1‑117 (MQ=255)
                                                                                                                                  tgCAATTTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGcc                     <  2:1078387/117‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGTCTGAACAGGCTTGCTGGCGCCAGTCCAGTTTCATTCAGTCGTGGTTTGGTTCTTACGGCCTGTGCAATCTACCTCATTAGGCACATCGGCCTGCCAGATACCGGCTCGGGGTGTATTTCCGCTTCCACGCTGAATACTGTTCTCAGCAATCCTGGGGTCATCACCTCTTCTGGTGTGCCTTGCGCCATAACATGTCCG  >  NC_000913/4510554‑4510819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: