| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,614,260 | G→A | *260* (TAG→TAA) | yjjV → | putative DNase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,614,260 | 0 | G | A | 98.1% | 162.7 / ‑4.2 | 53 | *260* (TAG→TAA) | yjjV | putative DNase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (28/24); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGT > NC_000913/4614138‑4614394 | ccTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑c > 2:1221644/1‑139 (MQ=255) cccGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCa < 2:312468/139‑1 (MQ=255) gCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGc < 1:437762/139‑1 (MQ=255) gtgtgtTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGcac < 1:56710/139‑1 (MQ=255) cGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGgcg < 2:35620/129‑1 (MQ=255) cGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGgcg > 1:35620/1‑129 (MQ=255) cGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGgcg > 2:1329330/1‑126 (MQ=255) cGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGgcg < 1:1329330/126‑1 (MQ=255) aGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa < 2:924546/139‑1 (MQ=255) aGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa < 1:47921/139‑1 (MQ=255) aGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa < 1:47920/139‑1 (MQ=255) ttGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcg > 2:48863/1‑139 (MQ=255) ccGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCt > 2:1096342/1‑139 (MQ=255) cggATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTa < 1:1221644/139‑1 (MQ=255) gagaTTGCGCAAGCGGTGCTAAATCACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGATTAACGCGTCTTATCATGCCTACAGGt < 1:881939/139‑1 (MQ=21) gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt > 1:111345/1‑117 (MQ=255) gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt < 2:111345/117‑1 (MQ=255) gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAcc > 2:1106387/1‑139 (MQ=255) gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAcc > 1:907524/1‑139 (MQ=255) gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg > 1:176833/1‑139 (MQ=255) ataaCACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa < 1:1115665/94‑1 (MQ=255) ataaCACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa > 2:1115665/1‑94 (MQ=255) ataaCACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAATCGTAGGCCGGa > 2:1043556/1‑139 (MQ=255) cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc < 1:250240/89‑1 (MQ=255) cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc > 2:250240/1‑89 (MQ=255) cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGttt < 1:1020569/113‑1 (MQ=255) cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGttt > 2:1020569/1‑113 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGcac > 1:100657/1‑64 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGcac < 2:100657/64‑1 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGcc > 2:1402218/1‑70 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGcc < 1:1402218/70‑1 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATc > 1:237755/1‑96 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATc < 2:237755/96‑1 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTa < 1:1290327/103‑1 (MQ=255) ataCGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTa > 2:1290327/1‑103 (MQ=255) gttgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc > 1:1177475/1‑86 (MQ=17) gttgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc < 2:1177475/86‑1 (MQ=17) tgttTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt > 2:1509039/1‑92 (MQ=255) tgttTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt < 1:1509039/92‑1 (MQ=255) gttTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc > 2:1169040/1‑79 (MQ=255) gttTAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc < 1:1169040/79‑1 (MQ=255) ttAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGc < 2:820604/67‑1 (MQ=255) ttAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGc > 1:820604/1‑67 (MQ=255) tAACGTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:1236130/1‑139 (MQ=255) gTGCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 2:1046651/1‑139 (MQ=255) gCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc < 1:51781/70‑1 (MQ=255) gCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc > 2:51781/1‑70 (MQ=255) gCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:117013/1‑139 (MQ=255) gCCGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:1335878/1‑139 (MQ=255) ccGTAGGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAg < 1:436517/139‑1 (MQ=40) cGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt < 2:620149/90‑1 (MQ=255) cGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt > 1:620149/1‑90 (MQ=255) cGTAAGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGt > 2:436517/1‑139 (MQ=255) | CCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTT‑‑CACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGT > NC_000913/4614138‑4614394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |