Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,101	C→T	*339* (TAG→TAA)	lplA ←	lipoate‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,101	0	C	T	100.0%	103.5 / NA	33	*339* (TAG→TAA)	lplA	lipoate‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

ATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGT  >  NC_000913/4622966‑4623227                                                                                                                                        |                                                                                                                               aTCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTAc                                                                                                                             >  2:1187881/1‑139 (MQ=255)         ggCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGc                                                                                                                     <  1:379199/139‑1 (MQ=255)         ggCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGc                                                                                                                     <  1:369198/139‑1 (MQ=255)            gcagcaGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGcccc                                                                                                                  >  2:1247015/1‑139 (MQ=255)              agcagTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCg                                                                                                                <  2:619316/139‑1 (MQ=255)                    tCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGccatcc                                                                                                          <  2:895620/139‑1 (MQ=255)                       ggCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATg                                                                                                       <  2:201881/139‑1 (MQ=255)                          aaaCGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCg                                                                                                    <  2:337114/139‑1 (MQ=255)                             cGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCg                                                                                                                >  1:166406/1‑124 (MQ=255)                             cGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCg                                                                                                                <  2:166406/124‑1 (MQ=255)                                              cgTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTa                                                                                                                         <  2:764293/98‑1 (MQ=255)                                              cgTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTa                                                                                                                         >  1:764293/1‑98 (MQ=255)                                              cgTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCtttt                                                                                >  1:818303/1‑139 (MQ=255)                                                      cGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTc                                                                        >  1:1520666/1‑139 (MQ=255)                                                         ggCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATg                                                                                                       >  2:1404498/1‑105 (MQ=255)                                                         ggCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATg                                                                                                       <  1:1404498/105‑1 (MQ=255)                                                           cGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGa                                                                   >  1:131209/1‑139 (MQ=255)                                                                     caTTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTcaaccaa                                                         <  2:1520666/139‑1 (MQ=255)                                                                               gAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCg                                               >  2:980379/1‑139 (MQ=255)                                                                                    gCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTc                                          >  2:821349/1‑139 (MQ=255)                                                                                        gAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGt                                      <  1:980379/139‑1 (MQ=255)                                                                                                     aaaTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTg                         <  2:610537/139‑1 (MQ=255)                                                                                                      aaTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTgc                        <  2:131209/139‑1 (MQ=255)                                                                                                      aaTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTgc                        <  2:1590120/139‑1 (MQ=255)                                                                                                      aaTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTgc                        <  1:821349/139‑1 (MQ=255)                                                                                                      aaTCGAAGAGAAAGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTgc                        <  1:1247015/139‑1 (MQ=255)                                                                                                                aaaGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAgg              >  2:114617/1‑139 (MQ=255)                                                                                                                  aGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGccatccat                                                                                                        <  2:492812/47‑1 (MQ=255)                                                                                                                  aGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGccatccat                                                                                                        >  1:492812/1‑47 (MQ=255)                                                                                                                  aGTTGCCCGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCa            >  2:915316/1‑139 (MQ=255)                                                                                                                        ccGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCggg                                                                    >  2:1434475/1‑77 (MQ=255)                                                                                                                        ccGCATGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCggg                                                                    <  1:1434475/77‑1 (MQ=255)                                                                                                                            aTGGGCGGGTAATTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAgt  >  1:563740/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                               ATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGT  >  NC_000913/4622966‑4623227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑