Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,168,350	Δ1 bp	coding (483/633 nt)	ycfQ ←	repressor for bhsA(ycfR)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,168,348	0	G	.	100.0%	79.7 / NA	20	coding (485/633 nt)	ycfQ	repressor for bhsA(ycfR)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (10/10);  total (10/10)

CAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAA  >  NC_000913/1168227‑1168481                                                                                                                          |                                                                                                                                      cAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCtt                                                                                                                     >  2:598843/1‑139 (MQ=255)                       aTCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAActgctg                                                                                               <  2:21080/139‑1 (MQ=255)                              tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                        <  1:598843/139‑1 (MQ=255)                                               tCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                     >  1:385547/1‑109 (MQ=255)                                               tCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                     <  2:385547/109‑1 (MQ=255)                                               tCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTgcgc                                                                                     >  2:621956/1‑125 (MQ=255)                                               tCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTgcgc                                                                                     <  1:621956/125‑1 (MQ=255)                                                   gTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGt                                                                   <  2:469817/139‑1 (MQ=255)                                                       gcTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGact                                                               >  1:545909/1‑137 (MQ=255)                                                                     ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCAt                                                                        >  2:720167/1‑116 (MQ=255)                                                                     ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCAt                                                                        <  1:720167/116‑1 (MQ=255)                                                                     ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCg                                                 >  2:473655/1‑139 (MQ=255)                                                                              taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                        >  2:236711/1‑139 (MQ=255)                                                                              taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                        >  1:335553/1‑139 (MQ=255)                                                                              taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                        >  1:277739/1‑139 (MQ=255)                                                                                         aaTTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga                             <  1:473655/139‑1 (MQ=255)                                                                                         aaTTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga                             <  2:471371/139‑1 (MQ=255)                                                                                                   gATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg                   <  2:335553/139‑1 (MQ=255)                                                                                                   gATGTGTCACGTCACAGTGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg                   <  1:236711/139‑1 (MQ=255)                                                                                                                    tGGGGC‑GGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGaa  >  2:102677/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                      CAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAA  >  NC_000913/1168227‑1168481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑