Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,782,393 (C)5→4 coding (334/4581 nt) ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,3890C.98.5% 275.5 / ‑3.2 67coding (338/4581 nt)ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (37/29);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAAC  >  NC_000913/2782252‑2782522
                                                                                                                                         |                                                                                                                                     
tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                      <  1:656840/139‑1 (MQ=255)
tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                      <  2:338690/139‑1 (MQ=255)
    accaTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgt                                                                                                                                  <  1:750279/139‑3 (MQ=255)
    accaTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgt                                                                                                                                  <  2:575609/139‑3 (MQ=255)
     ccaTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtc                                                                                                                                 <  2:341220/139‑4 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                             >  1:381014/1‑139 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                             >  1:608065/1‑139 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                             >  2:481517/1‑139 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                             >  2:487980/1‑139 (MQ=255)
            tGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAAtt                                                                                                                         <  1:200187/139‑1 (MQ=255)
                tATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACt                                                                                                                     >  1:262935/1‑139 (MQ=255)
                   tCCCTTCTCACTCCCCCCCCCTACGCCGGAAACAACGCCCTTAATTTTTGTTCCCTTTCTGATACCCCCTTTATAAACGTCCCGATTTCCACTCTCGATTTTCTTACCTGTGGAAATACCCCCTCATGAAATGACTGTc                                                                                                                  >  2:61343/1‑139 (MQ=255)
                   tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGACTTGACTGTc                                                                                                                  >  1:534523/1‑139 (MQ=255)
                   tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTc                                                                                                                  >  1:189308/1‑139 (MQ=255)
                      aTTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGc                                                                                                               <  2:761825/139‑1 (MQ=255)
                      aTTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGc                                                                                                               <  1:691984/139‑1 (MQ=255)
                       ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGcc                                                                                                              >  2:380100/1‑139 (MQ=255)
                       ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGcc                                                                                                              >  2:155864/1‑139 (MQ=255)
                          gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCAcc                                                                                                           >  1:31467/1‑139 (MQ=255)
                            aCTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTGTAAACGTCCGGTTTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                                <  2:578226/117‑5 (MQ=255)
                            aCTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                                >  1:578226/1‑113 (MQ=255)
                              tccctcccTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTa                                                                                                       <  1:237888/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                      >  1:111037/1‑139 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                      <  2:660233/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                      <  1:763158/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                      <  1:487980/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                      <  1:232146/139‑1 (MQ=255)
                                   ccctcTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGt                                                                                                  >  2:497253/1‑139 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGtt                                                                                                 <  2:150465/133‑1 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGtt                                                                                                 >  1:150465/1‑133 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                           >  1:155510/1‑139 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                           >  1:274892/1‑139 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                           >  2:688073/1‑139 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                           >  1:270749/1‑139 (MQ=255)
                                            cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                <  2:78772/132‑1 (MQ=255)
                                            cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                >  1:78772/1‑132 (MQ=255)
                                            cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTGAATGGTTGTattatt                                                                                         >  2:232119/1‑139 (MQ=255)
                                              tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAg                                                                                       >  2:612335/1‑139 (MQ=255)
                                               gAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                                                                                      <  2:608065/139‑1 (MQ=255)
                                                aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                     >  1:580849/1‑139 (MQ=255)
                                                       gCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                              >  1:631821/1‑139 (MQ=255)
                                                         ccTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCcaca                                                                            <  2:31467/139‑1 (MQ=255)
                                                                 gTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGtt                                                                    <  2:270749/139‑1 (MQ=255)
                                                                   cGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                  >  2:180565/1‑139 (MQ=255)
                                                                     ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                                <  2:739511/139‑1 (MQ=255)
                                                                     ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                                <  2:274892/139‑1 (MQ=255)
                                                                      tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                               <  1:180565/139‑1 (MQ=255)
                                                                         aTTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTc                                                            <  1:476734/139‑1 (MQ=255)
                                                                                  aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCtt                                                   >  2:845/1‑139 (MQ=255)
                                                                                    ccccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGa                                                 >  2:712827/1‑139 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                              >  1:528328/1‑139 (MQ=255)
                                                                                               aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                      <  1:688073/139‑1 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                   >  1:452590/1‑139 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAAATGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                   >  2:492459/1‑139 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGCGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACAAGATTc                                   >  2:198764/1‑139 (MQ=255)
                                                                                                    cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                          <  2:654881/114‑1 (MQ=255)
                                                                                                    cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                          >  1:654881/1‑114 (MQ=255)
                                                                                                    cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCaa                                 >  2:658917/1‑139 (MQ=255)
                                                                                                         tGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGa                            <  2:189308/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                           <  2:111037/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                           <  1:531037/139‑1 (MQ=255)
                                                                                                           ccACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACt                          <  1:492459/139‑1 (MQ=255)
                                                                                                            cACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACtt                         <  1:724443/139‑1 (MQ=255)
                                                                                                            cACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACtt                         <  1:593122/139‑1 (MQ=255)
                                                                                                                        tCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc             >  2:614833/1‑139 (MQ=255)
                                                                                                                           tACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCg          >  1:274153/1‑139 (MQ=255)
                                                                                                                               tgtgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                <  2:659703/49‑1 (MQ=255)
                                                                                                                               tgtgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                >  1:659703/1‑49 (MQ=255)
                                                                                                                               tgtgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCAt      <  1:481517/139‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                  >  1:510622/1‑76 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                  <  2:510622/76‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                                <  1:265419/78‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                                >  2:265419/1‑78 (MQ=255)
                                                                                                                                   gAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAAc  >  1:331146/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                     
TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAAC  >  NC_000913/2782252‑2782522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: