Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,860,426 Δ1 bp intergenic (+10/+41) pphB → / ← ygbI serine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,860,4250T.100.0% 166.4 / NA 40intergenic (+9/+42)pphB/ygbIserine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/18);  total (22/18)

CGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATA  >  NC_000913/2860296‑2860551
                                                                                                                                 |                                                                                                                              
cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGa                                                                                                                      >  1:59231/1‑139 (MQ=255)
  cTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAg                                                                                                                    >  2:710723/1‑139 (MQ=255)
           ttATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTaa                                                                                                           <  1:710723/139‑1 (MQ=255)
            tataTTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAc                                                                                                          <  1:621951/139‑1 (MQ=255)
              tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  1:220330/139‑1 (MQ=255)
               atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgtg                                                                                                                       >  1:88132/1‑123 (MQ=255)
               atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCg                                                                                                       >  2:568640/1‑139 (MQ=255)
               atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCAAGCTCTTCtgtg                                                                                                                       <  2:88132/123‑1 (MQ=255)
                   ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   <  2:59231/139‑1 (MQ=255)
                   ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   >  2:266160/1‑139 (MQ=255)
                        tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                              <  1:113987/139‑1 (MQ=255)
                                           cAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttttt                                                                           >  1:685416/1‑139 (MQ=255)
                                                gTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                      <  1:266477/139‑1 (MQ=255)
                                                  tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                    >  2:347567/1‑139 (MQ=255)
                                                   cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                         <  1:324517/101‑1 (MQ=255)
                                                   cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                         >  2:324517/1‑101 (MQ=255)
                                                   cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   >  1:29144/1‑107 (MQ=255)
                                                   cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   <  2:29144/107‑1 (MQ=255)
                                                   cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                   >  2:464592/1‑139 (MQ=255)
                                                             aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattat                                                                                   <  2:597027/113‑1 (MQ=255)
                                                             aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattat                                                                                   >  1:597027/1‑113 (MQ=255)
                                                             aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                         >  1:68252/1‑139 (MQ=255)
                                                                       aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgt                                                                                                                        >  1:322132/1‑66 (MQ=255)
                                                                       aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgt                                                                                                                        <  2:322132/66‑1 (MQ=255)
                                                                            ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        >  2:589050/1‑77 (MQ=255)
                                                                            ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  1:589050/77‑1 (MQ=255)
                                                                             gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgc                                         >  2:612132/1‑139 (MQ=255)
                                                                              aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                        >  2:243318/1‑139 (MQ=255)
                                                                                 gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcgc                                     <  1:568640/139‑1 (MQ=255)
                                                                                   cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGa                                   >  2:638772/1‑139 (MQ=255)
                                                                                       cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                            <  2:178839/110‑1 (MQ=255)
                                                                                       cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                            >  1:178839/1‑110 (MQ=255)
                                                                                          cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  2:262983/63‑1 (MQ=255)
                                                                                          cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                        >  1:262983/1‑63 (MQ=255)
                                                                                            ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCActc                          >  1:638234/1‑139 (MQ=255)
                                                                                                    tCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCaa                  >  1:184779/1‑139 (MQ=255)
                                                                                                            taaaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt          <  1:243318/139‑1 (MQ=255)
                                                                                                                 taaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGta     <  2:638234/139‑1 (MQ=255)
                                                                                                                   aaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaat   <  2:68252/139‑1 (MQ=255)
                                                                                                                    agtaatCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaata  >  1:679295/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                              
CGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATA  >  NC_000913/2860296‑2860551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: