| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,508,454 | Δ1 bp | coding (137/879 nt) | php ← | phosphotriesterase homology protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,508,451 | 0 | C | . | 93.5% | 111.7 / 2.4 | 31 | coding (140/879 nt) | php | phosphotriesterase homology protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base . (13/16); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
CCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAA > NC_000913/3508320‑3508584 | ccGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtc > 2:370242/1‑139 (MQ=255) gggAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcat > 1:362222/1‑139 (MQ=255) gAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcatca < 2:70648/139‑1 (MQ=255) aaaaCGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAgg < 1:453828/139‑1 (MQ=255) aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa < 1:370242/139‑1 (MQ=255) aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa < 1:559869/139‑1 (MQ=255) cacGTTGATCCCCGTCTCGCGCATTACTTCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCCTCAGCTCGTTCCTCTCCTGGCAAATGAACGCATACTGAt > 2:556569/1‑139 (MQ=255) cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATg < 1:291786/126‑1 (MQ=255) cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATg > 2:291786/1‑126 (MQ=255) cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt > 2:730075/1‑139 (MQ=255) ccccGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc < 2:511314/139‑1 (MQ=255) tACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGtttt < 2:362222/139‑1 (MQ=255) cAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAg < 1:405014/139‑1 (MQ=255) aTAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGga > 1:480647/1‑139 (MQ=255) agacggtgcagtgcgcCCCATGTAACGGTTGGTCATCTCAACCGCATTGCGTACG‑CCCGAGTCGTCAGGTCGTTGATCTCCTGGCAAATGAACTGATACTCATCATGGCGGCAGTCCACGTTGTTTTCAACGCCGgaga < 2:23474/132‑1 (MQ=255) gcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc < 1:652244/81‑1 (MQ=255) gcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc > 2:652244/1‑81 (MQ=255) aTGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCggg < 1:447241/42‑1 (MQ=255) aTGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCggg > 2:447241/1‑42 (MQ=255) aCGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATggg < 2:601513/139‑1 (MQ=255) ggTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg < 1:629727/139‑1 (MQ=255) ggTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg < 1:730075/139‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa < 1:110752/58‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa < 2:709091/58‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa > 2:110752/1‑58 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa > 1:709091/1‑58 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa > 2:421079/1‑66 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa < 1:421079/66‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGc < 2:703884/120‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGc > 1:703884/1‑120 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg > 2:344434/1‑139 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg > 2:319595/1‑139 (MQ=255) cgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaaa > 1:397770/1‑139 (MQ=255) cgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaaa > 2:713486/1‑139 (MQ=255) | CCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAA > NC_000913/3508320‑3508584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |