Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,190,043 (GA)4→5 coding (405/672 nt) phoP ← response regulator in two‑component regulatory system with PhoQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,190,0351.G100.0% 108.5 / NA 32R138P (CGC→CCC) phoPresponse regulator in two‑component regulatory system with PhoQ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/14);  total (18/14)
*NC_0009131,190,0352.A100.0% 100.8 / NA 32R138L (CGC→CTC) phoPresponse regulator in two‑component regulatory system with PhoQ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/14);  total (18/14)

AGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑GAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAG  >  NC_000913/1189897‑1190164
                                                                                                                                           ||                                                                                                                                 
aGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGc                                                                                                                                     <  1:724940/139‑1 (MQ=255)
  cTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑ga                                                                                                                                 >  2:622937/1‑139 (MQ=255)
            ggATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑gagagagagatc                                                                                                                       <  1:662252/139‑5 (MQ=255)
                       gAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACg                                                                                                              >  2:279370/1‑139 (MQ=255)
                            ttAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACgggggg                                                                                                         >  2:161078/1‑139 (MQ=255)
                                  aaTCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGaa                                                                                                   <  2:625065/139‑1 (MQ=255)
                                      tttGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGa                                                                                               <  2:189292/139‑1 (MQ=255)
                                         gCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCt                                                                                            >  1:168400/1‑139 (MQ=255)
                                               cACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAg                                                                                      <  2:263738/139‑1 (MQ=255)
                                                aCTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc                                                                                     <  1:417626/139‑1 (MQ=255)
                                                  tttGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCa                                                                                   >  1:519644/1‑139 (MQ=255)
                                                               cGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcg                                                                      <  1:325228/139‑1 (MQ=255)
                                                                                  taGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAg                                                                                  >  1:65805/1‑108 (MQ=255)
                                                                                  taGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAg                                                                                  <  2:65805/108‑1 (MQ=255)
                                                                                  taGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCAtt                                                   >  2:439753/1‑139 (MQ=255)
                                                                                     tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc                                                                                     >  1:466088/1‑102 (MQ=255)
                                                                                     tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc                                                                                     <  2:466088/102‑1 (MQ=255)
                                                                                     tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgc                                                >  1:229137/1‑139 (MQ=255)
                                                                                       tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc                                              >  2:768192/1‑139 (MQ=255)
                                                                                       tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc                                              >  2:401044/1‑139 (MQ=255)
                                                                                       tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc                                              >  1:214444/1‑139 (MQ=255)
                                                                                       tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc                                              >  1:250101/1‑139 (MQ=255)
                                                                                        aTTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc                                             <  1:599557/139‑1 (MQ=255)
                                                                                          tCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                           >  1:303637/1‑139 (MQ=255)
                                                                                          tCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGAGCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                           <  2:10198/139‑1 (MQ=255)
                                                                                             aaCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                        <  1:401044/139‑1 (MQ=255)
                                                                                             aaCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                        <  2:229137/139‑1 (MQ=255)
                                                                                             aaCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                        <  1:161078/139‑1 (MQ=255)
                                                                                              aCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑gagag                                                                                                                              <  2:266899/50‑1 (MQ=255)
                                                                                              aCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑gagag                                                                                                                              >  1:266899/1‑50 (MQ=255)
                                                                                               cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgcc                                                                    <  2:259099/109‑1 (MQ=255)
                                                                                               cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgcc                                                                    >  1:259099/1‑109 (MQ=255)
                                                                                               cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACct                                      >  1:11235/1‑139 (MQ=255)
                                                                                                                  tCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGttt                   >  1:648740/1‑139 (MQ=255)
                                                                                                                      cATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTc               <  1:759562/139‑1 (MQ=255)
                                                                                                                               aTAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatca      >  1:233544/1‑139 (MQ=255)
                                                                                                                                   ttCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGAACTGTGAAGCCAGACCGCTATTACGCCGCATTAATGTCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCAAATATTCATTAg  >  2:573994/1‑139 (MQ=255)
                                                                                                                                           ||                                                                                                                                 
AGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑GAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAG  >  NC_000913/1189897‑1190164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: