| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,920,205 | (T)7→8 | intergenic (+62/‑18) | yebT → / → rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,920,198 | 1 | . | T | 95.5% | 64.0 / ‑3.1 | 22 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (10/11); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920068‑1920330 | ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAGTGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tt‑tttttt > 1:464235/1‑138 (MQ=255)ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGAGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tt‑tttttt < 2:93525/139‑2 (MQ=255) gCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAg < 1:269490/139‑1 (MQ=255) cTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACa < 2:582087/139‑1 (MQ=255) ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGc > 1:638669/1‑139 (MQ=255) cTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCCTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTATTTTTGCCAGTGGTACATGCt < 2:464235/139‑1 (MQ=255) gAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCa > 1:392745/1‑139 (MQ=255) gTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAacac < 1:67272/139‑1 (MQ=255) gAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:674709/139‑1 (MQ=255) aCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCg < 2:566300/139‑1 (MQ=255) aaCTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGa < 2:392745/139‑1 (MQ=255) gcgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGAcaca > 1:284419/1‑139 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tt‑ttttttgcca < 1:65718/81‑6 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tt‑ttttttgcca > 2:65718/1‑76 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAg > 1:216018/1‑67 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAg < 2:216018/67‑1 (MQ=255) tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGGGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCg > 1:469964/1‑74 (MQ=255) tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCg < 2:469964/74‑1 (MQ=255) cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGct > 2:179998/1‑139 (MQ=255) gCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tt‑ttttttgcc > 2:19325/1‑48 (MQ=255) gCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tt‑ttttttgcc < 1:19325/52‑5 (MQ=255) cgcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAac > 2:194134/1‑67 (MQ=255) cgcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAac < 1:194134/67‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTc > 2:724028/1‑139 (MQ=255) aaCTGCGCGCCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCa < 1:179998/139‑1 (MQ=255) cgcgcCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAaca > 1:703024/1‑44 (MQ=255) cgcgcCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAaca < 2:703024/44‑1 (MQ=255) cgcCTGTTT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCg > 1:328756/1‑139 (MQ=255) | GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TT‑TTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920068‑1920330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |