Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,813	(C)7→8	coding (1005/1245 nt)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,806	1	.	C	100.0%	50.7 / NA	16	L338V (CTG→GTG)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (11/5);  total (11/5)

CCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAAC  >  NC_000913/3304670‑3304931                                                                                                                                          |                                                                                                                               ccccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑‑cc                                                                                                                             <  2:559075/139‑1 (MQ=255) ccccAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑‑cc                                                                                                                             <  2:242361/139‑1 (MQ=255)    cAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAAGAATCCGTTCGGGAACAACAG‑‑ccccc                                                                                                                          <  1:696591/139‑1 (MQ=255)                               cGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCCACAACTGGCGGGGCAAAGGTCAg                                                                                                 <  1:148856/139‑1 (MQ=255)                                                    aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACCGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGCGCAAAGGTCAGCAATGCCGTCTTCAAGCGGccc                                                                           >  2:56849/1‑139 (MQ=255)                                                    aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGccc                                                                           >  1:77370/1‑139 (MQ=255)                                                           ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCg                                                                    >  1:237202/1‑139 (MQ=255)                                                             ggAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                                                  <  2:355145/139‑1 (MQ=255)                                                                                   tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAAAAACAGC‑CCCCCCCACAACTAGCGGGGCAAAGGTCAGAAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTg                                            >  2:220861/1‑139 (MQ=255)                                                                                                  cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaaca                             >  1:89403/1‑139 (MQ=255)                                                                                                      aCCAATGGCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAcc                         <  2:280609/139‑1 (MQ=255)                                                                                                            ggCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAg                                                                                                      >  2:25485/1‑56 (MQ=255)                                                                                                            ggCGTACAGGAATCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAg                                                                                                      <  1:25485/56‑1 (MQ=255)                                                                                                                       aTCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGga        >  1:668123/1‑139 (MQ=255)                                                                                                                        tCCGTTCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaa       >  2:557706/1‑139 (MQ=255)                                                                                                                            ttCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaa   >  1:268824/1‑139 (MQ=255)                                                                                                                             tCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                 <  1:307762/60‑1 (MQ=255)                                                                                                                             tCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                 >  2:307762/1‑60 (MQ=255)                                                                                                                             tCGGGAACAACAGC‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaac  >  1:308344/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                               CCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑‑CCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAAC  >  NC_000913/3304670‑3304931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑