Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,511,746 (A)8→9 coding (599/906 nt) yhfZ ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,511,7381.A100.0% 102.9 / NA 33G203C (GGC→TGC) yhfZputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (21/12);  total (21/12)

GGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511602‑3511871
                                                                                                                                         |                                                                                                                                       
ggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aa                                                                                                                                     >  2:121643/1‑139 (MQ=255)
  cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aaaa                                                                                                                                   >  2:607545/1‑139 (MQ=255)
  cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aaaa                                                                                                                                   >  1:225781/1‑139 (MQ=255)
          ctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aaaaaaaaacat                                                                                                                           <  1:748256/139‑5 (MQ=255)
                             ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACACCGGTCATGATTTTCTGATc                                                                                                         >  2:82069/1‑139 (MQ=255)
                              tCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATcc                                                                                                        >  1:153531/1‑139 (MQ=255)
                                  tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  1:91431/139‑1 (MQ=255)
                                  tttCCGACACCACGCTCCAGATCACCGCATCGACCTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  2:709231/139‑1 (MQ=255)
                                                      aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                <  1:681651/139‑1 (MQ=255)
                                                      aTCAACGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                <  1:572188/139‑1 (MQ=255)
                                                           cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCa                                                                                                                      <  2:715274/96‑1 (MQ=255)
                                                           cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCa                                                                                                                      >  1:715274/1‑96 (MQ=255)
                                                           cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                           >  1:673891/1‑139 (MQ=255)
                                                                cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGt                                                                      >  1:690433/1‑139 (MQ=255)
                                                                  aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               >  1:579981/1‑112 (MQ=255)
                                                                  aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  2:579981/112‑1 (MQ=255)
                                                                     tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                                 >  2:437033/1‑139 (MQ=255)
                                                                                  tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  1:624372/1‑139 (MQ=255)
                                                                                  tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    <  2:225781/139‑1 (MQ=255)
                                                                                       tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa                                               <  1:560105/139‑1 (MQ=255)
                                                                                                   tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                                   >  2:601995/1‑139 (MQ=255)
                                                                                                   tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                                   >  2:438257/1‑139 (MQ=255)
                                                                                                        agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAAAACATCGGTCATGATTTTCTGTTCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 >  2:195454/1‑122 (MQ=255)
                                                                                                        agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 <  1:195454/122‑1 (MQ=255)
                                                                                                          agagaTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa                            <  1:121643/139‑1 (MQ=255)
                                                                                                          agagaTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa                            >  1:108194/1‑139 (MQ=255)
                                                                                                              aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                               <  2:652541/100‑1 (MQ=255)
                                                                                                              aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                               >  1:652541/1‑100 (MQ=255)
                                                                                                              aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                        >  1:370799/1‑139 (MQ=255)
                                                                                                                  aCTCGTTCCACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtg                    >  1:730625/1‑139 (MQ=255)
                                                                                                                         ccACATCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcc             >  1:195586/1‑139 (MQ=255)
                                                                                                                             aTCACTACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCaa         >  1:386377/1‑139 (MQ=255)
                                                                                                                                  tACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGTTccgccg                                                                                                    <  1:471804/43‑1 (MQ=255)
                                                                                                                                  tACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  2:471804/1‑43 (MQ=255)
                                                                                                                                  tACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTGACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa    >  1:478699/1‑139 (MQ=255)
                                                                                                                                  tACCGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa    >  1:137020/1‑139 (MQ=255)
                                                                                                                                    ccGCCA‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  2:308286/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511602‑3511871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: