Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,435	T→C	T395A (ACT→GCT)	ylaB ←	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,435	0	T	C	100.0%	137.7 / NA	40	T395A (ACT→GCT)	ylaB	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

ATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCG  >  NC_000913/477310‑477542                                                                                                                              |                                                                                                             aTCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGcgcg                                                                                                 >  1:651570/1‑139 (MQ=255)   ccTGTAAATAACTTAAACTTGAATACCCCTTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                               <  2:497351/139‑1 (MQ=255)      gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctg                                                                                            >  2:129797/1‑139 (MQ=255)      gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctg                                                                                            >  2:607389/1‑139 (MQ=255)       tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                           >  2:653094/1‑139 (MQ=255)       tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                           >  2:315163/1‑139 (MQ=255)       tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                           >  2:533368/1‑139 (MQ=255)       tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                           >  1:698279/1‑139 (MQ=255)       tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                           >  1:358897/1‑139 (MQ=255)        aaaTAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                          <  2:651570/139‑1 (MQ=255)        aaaTAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                          <  1:533368/139‑1 (MQ=255)                    cTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCt                                                                             <  2:362796/139‑1 (MQ=255)                        aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATa                                                                         <  2:453139/139‑1 (MQ=255)                         aTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGCTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAg                                                                        >  1:185378/1‑139 (MQ=255)                             cccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGtgat                                                                    >  1:432324/1‑139 (MQ=255)                                 tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTGAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa                                                                           <  1:515689/128‑1 (MQ=255)                                 tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa                                                                           >  2:515689/1‑128 (MQ=255)                                 tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGtgattgat                                                                >  2:410687/1‑139 (MQ=255)                                  aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                               <  1:588577/108‑1 (MQ=255)                                  aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                               >  2:588577/1‑108 (MQ=255)                                      aacgcatcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTGCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGt                                                           >  2:496142/5‑139 (MQ=255)                                            tcaAGATAAGTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCATCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa                                                                           <  2:164473/117‑1 (MQ=255)                                            tcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa                                                                           >  1:164473/1‑117 (MQ=255)                                             caAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                               <  1:444933/97‑1 (MQ=255)                                             caAGATAAATTTCATGGCCCGCCTCCCGGAAGCGAGAAATTATCGAGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                               >  2:444933/1‑97 (MQ=255)                                                    aaTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGc                                             <  2:432324/139‑1 (MQ=255)                                                           tGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAttt                                      >  1:270676/1‑139 (MQ=255)                                                                     cccGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGt                            <  1:374162/139‑1 (MQ=255)                                                                            gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGCTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACg                     <  2:185378/139‑1 (MQ=255)                                                                              gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTTCGAGGTGAGCACggg                   <  1:653094/139‑1 (MQ=255)                                                                              gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg                   <  1:129797/139‑1 (MQ=255)                                                                                         tggtctcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGGTCAAGCGGGAGCT‑TCAGAGGTGTAACCTGGTAGTGATTGATTATGTCACGCAGCAACTGCAGGATTTTTACCGAGGTGAGCACGGGCAATTCGAGAt        <  2:28189/133‑1 (MQ=255)                                                                                            gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt                                                                                                         >  2:103681/1‑40 (MQ=255)                                                                                            gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt                                                                                                         <  1:103681/40‑1 (MQ=255)                                                                                            gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTg‑g                                                                                      >  1:656346/1‑58 (MQ=255)                                                                                            gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTg‑g                                                                                      <  2:656346/58‑1 (MQ=255)                                                                                             cgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGAt    <  2:259736/139‑1 (MQ=255)                                                                                               cTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCg  <  1:438042/139‑1 (MQ=255)                                                                                                            cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGAtt                                                                                  >  1:685234/1‑46 (MQ=255)                                                                                                            cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGAtt                                                                                  <  2:685234/46‑1 (MQ=255)                                                                                                                              |                                                                                                             ATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCG  >  NC_000913/477310‑477542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑