Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	490,285	C→T	*176* (TAG→TAA)	priC ←	primosomal replication protein N''

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	490,285	0	C	T	100.0%	68.6 / NA	23	*176* (TAG→TAA)	priC	primosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/10);  total (13/10)

CGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGA  >  NC_000913/490156‑490417                                                                                                                                  |                                                                                                                                     cGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGtt                                                                                                                             >  1:702639/1‑139 (MQ=255)    tttACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaaa                                                                                                                          <  2:714472/139‑1 (MQ=255)            atCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTaa                                                                                                                  >  2:725057/1‑139 (MQ=255)            atCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTaa                                                                                                                  >  2:275084/1‑139 (MQ=255)                aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTg                                                                                                              >  1:441078/1‑139 (MQ=255)                aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTg                                                                                                              >  1:402002/1‑139 (MQ=255)                aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTg                                                                                                              >  1:587366/1‑139 (MQ=255)                                          gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcg                                                                                    >  2:118540/1‑139 (MQ=255)                                                            cagcaCAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcgc                                                                  >  1:90367/1‑139 (MQ=255)                                                                acaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                              <  2:402002/139‑1 (MQ=255)                                                                  aaTCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCCTa                                                            >  1:567553/1‑139 (MQ=255)                                                                     cAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGTGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCAAGGCGCGCTTCATAGGc                                                         <  2:441078/139‑1 (MQ=255)                                                                       aaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCtt                                                       <  1:571269/139‑1 (MQ=255)                                                                            aCAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACt                                                  >  2:252081/1‑139 (MQ=255)                                                                            aCAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACt                                                  <  2:702639/139‑1 (MQ=255)                                                                                    tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATg                                          <  1:637811/139‑1 (MQ=255)                                                                                                aGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTg                              <  2:90367/139‑1 (MQ=255)                                                                                                       ttttCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                              >  1:763218/1‑100 (MQ=255)                                                                                                       ttttCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                              <  2:763218/100‑1 (MQ=255)                                                                                                        tttCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACga                      >  2:531111/1‑139 (MQ=255)                                                                                                                     atTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTGTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATCGGCTTCCACTTCACGATGCAGCGTTTACTGTTCCACGAGATCGTTCACCcg         <  1:471938/139‑1 (MQ=255)                                                                                                                         tctcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGcc     >  2:336861/1‑139 (MQ=255)                                                                                                                            ccATTGTTAGCGGGTTAAACGCGCTAACCTTTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa  <  1:531111/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGA  >  NC_000913/490156‑490417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑