Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,724	C→T	A59T (GCG→ACG)	ybfE ←	LexA‑regulated protein, CopB family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,724	0	C	T	100.0%	108.0 / NA	34	A59T (GCG→ACG)	ybfE	LexA‑regulated protein, CopB family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

ACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCT  >  NC_000913/711589‑711849                                                                                                                                        |                                                                                                                              aCATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGtt                                                                                                                            <  2:712684/139‑1 (MQ=255)     gAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGc                                                                                                                        >  2:720185/1‑139 (MQ=255)     gAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGc                                                                                                                        >  1:56808/1‑139 (MQ=255)       aaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCtt                                                                                                                      >  2:173112/1‑139 (MQ=255)       aaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCtt                                                                                                                      >  2:302984/1‑139 (MQ=255)                            ccTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGcc                                                                                                 <  1:173112/139‑1 (MQ=255)                                aCTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGt                                                                                             <  2:56808/139‑1 (MQ=255)                                 cTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGACTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTa                                                                                            >  1:489364/1‑139 (MQ=255)                                     gCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACttt                                                                                        >  1:635738/1‑139 (MQ=255)                                     gCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACttt                                                                                        >  1:727326/1‑139 (MQ=255)                                          gccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTcgc                                                                                   >  2:291989/1‑139 (MQ=255)                                                 gTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTaa                                                                                                     <  1:503829/114‑1 (MQ=255)                                                 gTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTaa                                                                                                     >  2:503829/1‑114 (MQ=255)                                                          ttAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTGCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCg                                                                   <  2:413439/139‑1 (MQ=255)                                                                    ttCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATa                                                         >  2:150028/1‑139 (MQ=255)                                                                    ttCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATa                                                         >  2:263331/1‑139 (MQ=255)                                                                      cGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATa                                                         >  2:306037/1‑137 (MQ=255)                                                                      cGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATa                                                         <  1:306037/137‑1 (MQ=255)                                                                      cGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                       <  1:263331/139‑1 (MQ=255)                                                                             ttCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                             >  1:728670/1‑78 (MQ=255)                                                                             ttCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGac                                                                                                             <  2:728670/78‑1 (MQ=255)                                                                                gCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCa                                             <  2:727326/139‑1 (MQ=255)                                                                                gCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCa                                             <  2:635738/139‑1 (MQ=255)                                                                                       cacATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCgtgt                                                                                                                             <  1:327119/50‑1 (MQ=255)                                                                                       cTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCgtgt                                                                                                                             >  2:327119/1‑52 (MQ=255)                                                                                       cTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATcgcgcg                                      >  2:436566/1‑139 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTgg                                                                        >  1:689237/1‑96 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTgg                                                                        <  2:689237/96‑1 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                       >  1:197643/1‑113 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                       <  2:197643/113‑1 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                       <  1:487748/113‑1 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                             >  1:639278/1‑139 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGAt                             >  1:380391/1‑139 (MQ=255)                                                                                                cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACATTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACg                                                       >  2:487748/1‑113 (MQ=255)                                                                                                    aCTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGt                         >  2:7563/1‑139 (MQ=255)                                                                                                                           tCGACAGCTTCCGTGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCt  <  2:700188/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                              ACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCT  >  NC_000913/711589‑711849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑