Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	716,388	C→T	pseudogene (210/651 nt)	ybfG ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	716,388	0	C	T	100.0%	125.6 / NA	40	pseudogene (210/651 nt)	ybfG	pseudogene
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

TTAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAACTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTTG  >  NC_000913/716257‑716521                                                                                                                                    |                                                                                                                                      ttAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCAt                                                                                                                                <  2:708877/139‑1 (MQ=255)      agCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatca                                                                                                                           <  1:15451/139‑1 (MQ=255)        cAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatca                                                                                                                           <  2:703988/137‑1 (MQ=255)        cAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatca                                                                                                                           >  1:703988/1‑137 (MQ=255)         aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                        >  2:365596/1‑139 (MQ=255)                     tGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCg                                                                                                            <  2:258961/139‑1 (MQ=255)                     tGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCg                                                                                                            <  1:631542/139‑1 (MQ=255)                       cGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGa                                                                                                          >  2:13637/1‑139 (MQ=255)                             cGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATa                                                                                                    >  1:185223/1‑139 (MQ=255)                                       gggTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCa                                                                                          <  2:752055/139‑1 (MQ=255)                                             aGCCCGCAGGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTTGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCACTATCAATCAGCAGACCTCCGGCGCCATAGACAAGCGCACCCATc                                                                                    >  2:318168/1‑139 (MQ=255)                                                  gCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGa                                                                               >  1:638861/1‑139 (MQ=255)                                                  gCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGa                                                                               >  1:318773/1‑139 (MQ=255)                                                          gCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTttgttg                                                                       <  1:365596/139‑1 (MQ=255)                                                           cGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTtgttgt                                                                      <  1:714378/139‑1 (MQ=255)                                                           cGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTtgttgt                                                                      <  2:185223/139‑1 (MQ=255)                                                              aTTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCAc                                                                   <  2:615413/139‑1 (MQ=255)                                                                   gggCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTa                                                              >  2:655126/1‑139 (MQ=255)                                                                         tgtgTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTttgtt                                                                        <  1:60572/123‑1 (MQ=255)                                                                         tgtgTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTttgtt                                                                        >  2:60572/1‑123 (MQ=255)                                                                                            ctcGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTaa                                     >  2:609496/1‑139 (MQ=255)                                                                                              cGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCgg                                                                                                           >  1:561183/1‑67 (MQ=255)                                                                                              cGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCgg                                                                                                           <  2:561183/67‑1 (MQ=255)                                                                                               gTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTCGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATc                                  >  1:124708/1‑139 (MQ=255)                                                                                                      ccGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTttatta                           <  1:609496/139‑1 (MQ=255)                                                                                                         ggggTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAAcc                        >  1:354259/1‑139 (MQ=255)                                                                                                             tGACCAGATCCTGCTATGCGTAATTAACCATTATCAATcagca                                                                                                                    <  2:495437/43‑1 (MQ=255)                                                                                                             tGACCAGATCCTGCTATGCGTAATTAACCATTATCAATcagca                                                                                                                    >  1:495437/1‑43 (MQ=255)                                                                                                              gACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTaa                   <  2:638861/139‑1 (MQ=255)                                                                                                               aCCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAag                  <  2:354259/139‑1 (MQ=255)                                                                                                                  aGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGt               <  2:318773/139‑1 (MQ=255)                                                                                                                   gATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGtt              <  2:279511/139‑1 (MQ=255)                                                                                                                     tCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGt                                                               <  2:264938/88‑1 (MQ=255)                                                                                                                     tCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGt                                                               >  1:264938/1‑88 (MQ=255)                                                                                                                       cTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGt          <  1:369791/139‑1 (MQ=255)                                                                                                                         gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTAt        >  1:675782/1‑139 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt   >  2:655919/1‑139 (MQ=255)                                                                                                                               cGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTtg  >  2:533207/1‑139 (MQ=255)                                                                                                                               cGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTtg  >  2:26784/1‑139 (MQ=255)                                                                                                                               cGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTtg  >  2:678475/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      TTAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAACTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTTG  >  NC_000913/716257‑716521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑