Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	826,119	C→T	*378* (TAG→TAA)	ybhS ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	826,119	0	C	T	100.0%	64.8 / NA	22	*378* (TAG→TAA)	ybhS	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

GCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTT  >  NC_000913/825987‑826239                                                                                                                                     |                                                                                                                         gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATcc                                                                                                                    >  2:451082/1‑139 (MQ=255)               cAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGt                                                                                                      <  1:430478/139‑1 (MQ=255)                      aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                               <  1:451082/139‑1 (MQ=255)                          gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGTATCTCAGCCACg                                                                                           >  2:716496/1‑139 (MQ=255)                              tAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAg                                                                                       <  2:735089/139‑1 (MQ=255)                              tAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAg                                                                                       <  2:599732/139‑1 (MQ=255)                                     ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                >  1:287913/1‑139 (MQ=255)                                     ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                >  2:405974/1‑139 (MQ=255)                                          aaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGGCGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                           <  1:405974/139‑1 (MQ=255)                                          aaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                           <  2:217671/139‑1 (MQ=255)                                                                    cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGATGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                 <  2:767976/139‑1 (MQ=255)                                                                    cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                 >  2:157466/1‑139 (MQ=255)                                                                    cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                 >  2:27313/1‑139 (MQ=255)                                                                    cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                 >  1:70863/1‑139 (MQ=255)                                                                    cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                 <  1:568784/139‑1 (MQ=255)                                                                                            tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGtt                                                                                                          >  2:314032/1‑58 (MQ=255)                                                                                            tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGtt                                                                                                          <  1:314032/58‑1 (MQ=255)                                                                                                  ttAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGTCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                    >  2:48317/1‑122 (MQ=255)                                                                                                  ttAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGTCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                    <  1:48317/122‑1 (MQ=255)                                                                                                             aaGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc        <  1:27313/139‑1 (MQ=255)                                                                                                               gCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg      <  2:133865/139‑1 (MQ=255)                                                                                                                   tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt  >  2:83553/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                         GCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTT  >  NC_000913/825987‑826239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑