Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	879,857	G→A	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI → gstB ←	soluble aldose sugar dehydrogenase glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	879,857	0	G	A	100.0%	127.0 / NA	40	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI gstB	soluble aldose sugar dehydrogenase glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/20);  total (20/20)

AAGTGACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879721‑879987                                                                                                                                         |                                                                                                                                   aaGTGACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACt                                                                                                                                  <  1:642820/139‑1 (MQ=255)     gACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACg                                                                                                                              <  1:450316/139‑1 (MQ=255)                         tAACGGACAGAGGGCAGCGAATTCGTGATCTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          <  1:521799/139‑1 (MQ=255)                                   agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                <  1:674597/139‑1 (MQ=255)                                     ggCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                              >  1:29532/1‑139 (MQ=255)                                            aaTTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTg                                                                                       >  2:331992/1‑139 (MQ=255)                                                                 aCCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGc                                                                  >  2:151020/1‑139 (MQ=255)                                                                   ccGACGGTTATTTATACGTTCACACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAg                                                                >  2:206975/1‑139 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGTAcgcg                                                     <  2:87160/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:263939/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:169852/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:331992/139‑1 (MQ=255)                                                                                   cGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTc                                                <  2:29532/139‑1 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcgc                                                                                                         <  1:330407/79‑1 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcgc                                                                                                         >  2:330407/1‑79 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           <  1:257978/77‑1 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           >  2:257978/1‑77 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCGTCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                            >  2:501621/1‑139 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:507184/130‑1 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:507184/1‑130 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                            >  1:370073/1‑139 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                            <  2:305329/139‑1 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                            >  2:682484/1‑139 (MQ=255)                                                                                        tcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           >  1:636179/1‑139 (MQ=255)                                                                                               cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCa                                    >  1:582499/1‑139 (MQ=255)                                                                                                  gTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACg                                 >  1:108391/1‑139 (MQ=255)                                                                                                            gAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAAt                       >  1:663221/1‑139 (MQ=255)                                                                                                               ttACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGt                    >  2:570169/1‑139 (MQ=255)                                                                                                                      aaGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGaa             <  1:279086/139‑1 (MQ=255)                                                                                                                      aaGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGaa             <  2:582499/139‑1 (MQ=255)                                                                                                                      aaGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGaa             <  2:697918/139‑1 (MQ=255)                                                                                                                         ttAGCCCACTCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        <  2:476191/61‑1 (MQ=255)                                                                                                                         ttAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        >  1:476191/1‑61 (MQ=255)                                                                                                                            gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:566085/1‑93 (MQ=255)                                                                                                                            gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:566085/93‑1 (MQ=255)                                                                                                                             cccACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAg                                                                                     >  1:685165/1‑60 (MQ=255)                                                                                                                             cccACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAg                                                                                     <  2:685165/60‑1 (MQ=255)                                                                                                                                 cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatag  >  1:661593/1‑139 (MQ=255)                                                                                                                                  gCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:531673/87‑1 (MQ=255)                                                                                                                                  gCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:531673/1‑87 (MQ=255)                                                                                                                                         |                                                                                                                                   AAGTGACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879721‑879987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑