Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 922,366 C→T *75* (TAG→TAA cspD ← inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3660CT100.0% 64.1 / NA 23*75* (TAG→TAAcspDinhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/8);  total (15/8)

ACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGAAT  >  NC_000913/922239‑922499
                                                                                                                               |                                                                                                                                     
aCCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGc                                                                                                                            <  1:278575/139‑1 (MQ=255)
          aaTGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACt                                                                                                                  >  2:367973/1‑139 (MQ=255)
            tGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCAGCTTCTACTTc                                                                                                                >  1:499906/1‑139 (MQ=255)
                gCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                            <  2:499906/139‑1 (MQ=255)
                                         tGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGGGATTGc                                                                                   >  2:304909/1‑139 (MQ=255)
                                          ggCACGAGAGTAATTGTTGAGATCAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGAAGTACACAATGAGAAAGCAGAGTTATACGACTGCCGCCTCTACTTCGACAGGCACAATAACACCGGCGAGATTGcc                                                                                  >  2:180190/1‑139 (MQ=255)
                                                            gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGAc                                                                >  1:733144/1‑139 (MQ=255)
                                                              gaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACAt                                                              >  1:317434/1‑139 (MQ=255)
                                                                           gCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                            >  1:338227/1‑80 (MQ=255)
                                                                           gCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                            <  2:338227/80‑1 (MQ=255)
                                                                                                                 aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa           <  1:396692/139‑1 (MQ=255)
                                                                                                                  tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAGTAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAt          >  1:431504/1‑139 (MQ=255)
                                                                                                                  tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGTCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa           >  1:404730/1‑138 (MQ=255)
                                                                                                                  tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGTCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa           <  2:404730/138‑1 (MQ=255)
                                                                                                                     gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCg                                                                                                               >  2:540309/1‑35 (MQ=255)
                                                                                                                     gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCg                                                                                                               <  1:540309/35‑1 (MQ=255)
                                                                                                                     gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtggt       >  2:122817/1‑139 (MQ=255)
                                                                                                                          aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt                                                                    <  1:530874/73‑1 (MQ=255)
                                                                                                                          aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt                                                                    >  2:530874/1‑73 (MQ=255)
                                                                                                                          aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtg         <  2:379443/132‑1 (MQ=255)
                                                                                                                          aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtg         >  1:379443/1‑132 (MQ=255)
                                                                                                                          aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGaat  >  2:141377/1‑139 (MQ=255)
                                                                                                                          aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGaat  >  2:379540/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                     
ACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGAAT  >  NC_000913/922239‑922499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: