Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,499	T→C	C293C (TGT→TGC)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,499	0	T	C	100.0%	74.2 / NA	22	C293C (TGT→TGC)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

TGCTGAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATC  >  NC_000913/1148375‑1148633                                                                                                                             |                                                                                                                                       tgctgAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTgcg                                                                                                                          <  2:74647/139‑1 (MQ=255) tgctgAAATCACAGGGTGAATGGAAAAAACGGTCGTGGTGGCTACTGTTTTTAAAGCGTTGGCTACAAAAGAGCCTGACGATGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTgcg                                                                                                                          >  1:656269/1‑139 (MQ=255)   ctgAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGcgcg                                                                                                                        >  1:677812/1‑139 (MQ=255)      aaaTCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCa                                                                                                                     <  1:315514/139‑1 (MQ=255)           tcAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTg                                                                                                                <  2:753416/139‑1 (MQ=255)                                                ttattaAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTg                                                                           >  2:438636/1‑139 (MQ=255)                                                          gTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCa                                                                                                                     >  1:512936/1‑87 (MQ=255)                                                          gTTGGCTACAAAACAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCa                                                                                                                     <  2:512936/87‑1 (MQ=255)                                                            tGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTg                                                               >  1:668100/1‑139 (MQ=255)                                                             ggCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGa                                                              <  1:210780/139‑1 (MQ=255)                                                                        agCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGc                                                   >  1:692122/1‑139 (MQ=255)                                                                        agCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGc                                                   >  2:479884/1‑139 (MQ=255)                                                                          ccTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAg                                                 >  2:325074/1‑139 (MQ=255)                                                                             gACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCg                                              >  2:710088/1‑139 (MQ=255)                                                                                                        cGACCAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAAtt                   >  1:282852/1‑139 (MQ=255)                                                                                                            cAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCg               <  1:479884/139‑1 (MQ=255)                                                                                                            cAGTATAACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCg               <  2:668100/139‑1 (MQ=255)                                                                                                               tataACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCg               <  1:483506/136‑1 (MQ=255)                                                                                                               tataACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCg               >  2:483506/1‑136 (MQ=255)                                                                                                               tataACGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTc            >  1:621121/1‑139 (MQ=255)                                                                                                                  aaCGGCGCCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGcc         <  2:693590/139‑1 (MQ=255)                                                                                                                      gcgcCTGCCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCACAGCGAATTGCCGCTCGCCTGGa     <  2:282852/139‑1 (MQ=255)                                                                                                                       cgcCTGCCTGTTAGGATTGCGCGGCAAGGTGAGAAAAAGGCGTGAGGTGTGCATCAAACGAGGCTTTGCGGTAGTGATTAAACAGGGCTTGCAGGCGGTGCAGCCTTAAGTTCTTCAGCGAATTGGCGCACGCCTTGag    <  2:656269/139‑2 (MQ=255)                                                                                                                         cctgcctgTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAg                                 <  1:600338/108‑1 (MQ=255)                                                                                                                         cctgcctgTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAg                                 >  2:600338/1‑108 (MQ=255)                                                                                                                         cctgcctgTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGa     >  2:110734/1‑136 (MQ=255)                                                                                                                         cctgcctgTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGa     <  1:110734/136‑1 (MQ=255)                                                                                                                         cctgcctgTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATc  >  1:48181/1‑139 (MQ=255)                                                                                                                         cctgcctgTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATc  >  2:322486/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       TGCTGAAATCTCAGGGTGAAGGGAAAAAACGGTCGTGGTGGCTACTGTTATTAAAGCGTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATC  >  NC_000913/1148375‑1148633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑