Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,208,517 C→T P129L (CCT→CTT) 
*201* (TAG→TAA
tfaP →
tfaE ←
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,208,5170CT100.0% 99.8 / NA 35P129L (CCT→CTT) 
*201* (TAG→TAA
tfaP
tfaE
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/13);  total (22/13)

TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCA  >  NC_000913/1208383‑1208650
                                                                                                                                      |                                                                                                                                     
tAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAGACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACg                                                                                                                                   >  2:509539/1‑139 (MQ=255)
  gCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGcc                                                                                                                                 >  1:727186/1‑139 (MQ=255)
           ggCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGt                                                                                                                        >  2:571191/1‑139 (MQ=255)
                          cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACAt                                                                                                        >  2:179093/1‑139 (MQ=255)
                                gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                  <  1:325779/139‑1 (MQ=255)
                                gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCGCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                  <  2:438487/139‑1 (MQ=255)
                                  aGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                                <  1:509539/139‑1 (MQ=255)
                                    aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                              >  2:556227/1‑139 (MQ=255)
                                    aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                              <  2:405736/139‑1 (MQ=255)
                                     cTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTg                                                                                             <  2:19534/139‑1 (MQ=255)
                                              gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                                    >  2:645998/1‑139 (MQ=255)
                                                tGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGt                                                                                  >  1:350597/1‑139 (MQ=255)
                                                                 aTTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCaa                                                                 >  1:732892/1‑139 (MQ=255)
                                                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAAt                                                           >  1:472537/1‑139 (MQ=255)
                                                                                  cTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCg                                                <  1:167160/139‑1 (MQ=255)
                                                                                      aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTaa                                            >  1:110378/1‑139 (MQ=255)
                                                                                           cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                  >  1:153799/1‑80 (MQ=17)
                                                                                           cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                  <  2:153799/80‑1 (MQ=17)
                                                                                           cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTCGTATCTGTTGCCGTCATCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTg                                       >  2:514804/1‑139 (MQ=14)
                                                                                            tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTgg                                      <  2:110378/139‑1 (MQ=255)
                                                                                                    gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTct                              >  2:366067/1‑139 (MQ=255)
                                                                                                        aTACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                             <  2:396738/41‑1 (MQ=255)
                                                                                                        aTACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                             >  1:396738/1‑41 (MQ=255)
                                                                                                         tACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCaa                                                                 <  2:349666/99‑1 (MQ=255)
                                                                                                         tACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCaa                                                                 >  1:349666/1‑99 (MQ=255)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAg                                                                                                               <  2:696213/48‑1 (MQ=11)
                                                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAg                                                                                                               >  1:696213/1‑48 (MQ=11)
                                                                                                               cAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                         >  2:697170/1‑85 (MQ=11)
                                                                                                               cAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                         <  1:697170/85‑1 (MQ=255)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTg                                                                                             <  2:714350/62‑1 (MQ=11)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTg                                                                                             >  1:714350/1‑62 (MQ=11)
                                                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt                >  2:149513/1‑139 (MQ=255)
                                                                                                                   gcgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTa               >  1:344280/1‑139 (MQ=255)
                                                                                                                      ccAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGt            >  2:690779/1‑139 (MQ=255)
                                                                                                                                aaTGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCa  >  1:659021/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCA  >  NC_000913/1208383‑1208650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: