Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,225,089 T→C T91T (ACA→ACG minD ← inhibitor of FtsZ ring polymerization, chromosome‑membrane tethering protein, membrane ATPase of the MinCDEE system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,225,0890TC100.0% 89.2 / NA 26T91T (ACA→ACGminDinhibitor of FtsZ ring polymerization, chromosome‑membrane tethering protein, membrane ATPase of the MinCDEE system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/13);  total (13/13)

TGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAAT  >  NC_000913/1224966‑1225217
                                                                                                                           |                                                                                                                                
tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGc                                                                                                                   >  1:58245/1‑139 (MQ=255)
 gCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCa                                                                                                                  <  2:710140/139‑1 (MQ=255)
   cATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGa                                                                                                                <  2:716108/139‑1 (MQ=255)
     ttAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAat                                                                                                              >  1:178280/1‑139 (MQ=255)
            aCCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAgaga                                                                                                       >  1:28202/1‑139 (MQ=255)
                     aaTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTa                                                                                              <  2:28202/139‑1 (MQ=255)
                         ccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt                                                                                          <  2:321764/139‑1 (MQ=255)
                         ccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt                                                                                          <  2:178280/139‑1 (MQ=255)
                                                 aaTTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTa                                                                  >  1:379867/1‑139 (MQ=255)
                                                       aaaTCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTg                                                            >  1:362302/1‑139 (MQ=255)
                                                                           atcaAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTg                                        >  1:259046/1‑139 (MQ=255)
                                                                             caAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGAc                                      <  2:362302/139‑1 (MQ=255)
                                                                              aaGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACg                                     <  2:259046/139‑1 (MQ=255)
                                                                                gAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGaa                                   >  1:496541/1‑139 (MQ=255)
                                                                                      tGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt                              >  1:597799/1‑138 (MQ=255)
                                                                                      tGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt                              <  2:597799/138‑1 (MQ=255)
                                                                                                       gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGAt                                                                                                      <  2:525163/49‑1 (MQ=255)
                                                                                                       gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac            <  1:81421/139‑1 (MQ=255)
                                                                                                       gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac            <  2:379867/139‑1 (MQ=255)
                                                                                                       gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac            <  2:770951/139‑1 (MQ=255)
                                                                                                       gTGAGGGCATCTTTATAGCGCGTTTGCGATGCCGGCAGAATATAGAGAt                                                                                                      >  1:525163/1‑49 (MQ=255)
                                                                                                          aGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATTTAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAAc         >  2:82502/1‑139 (MQ=255)
                                                                                                          aGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAAc         >  2:86708/1‑139 (MQ=255)
                                                                                                            ggCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGAc                       >  1:754787/1‑123 (MQ=255)
                                                                                                            ggCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGAc                       <  2:754787/123‑1 (MQ=255)
                                                                                                                 cTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAAt  >  2:168718/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                                
TGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAAT  >  NC_000913/1224966‑1225217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: