Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,403,059 A→G I66T (ATT→ACT)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,403,0590AG100.0% 94.1 / NA 27I66T (ATT→ACT) abgBp‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

TGTCCGCAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAATAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATA  >  NC_000913/1402924‑1403193
                                                                                                                                       |                                                                                                                                      
tgtCCGCAACCGAGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTaa                                                                                                                                     <  1:127100/139‑1 (MQ=255)
                   tttCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAAGCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGcc                                                                                                                  >  2:292537/1‑139 (MQ=255)
                                         aGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAg                                                                                            <  2:90670/139‑1 (MQ=255)
                                          ggTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGc                                                                                           >  2:650848/1‑139 (MQ=255)
                                               cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         <  2:83413/120‑1 (MQ=255)
                                               cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg                                                                                                         >  1:83413/1‑120 (MQ=255)
                                               cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCa                                                                                      >  1:613618/1‑139 (MQ=255)
                                                                           gCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCg                                                          >  1:760013/1‑139 (MQ=255)
                                                                                      atatTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAAc                                               >  1:39418/1‑139 (MQ=255)
                                                                                                  cagGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACgt                                   <  1:292537/139‑1 (MQ=255)
                                                                                                   agGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACgtg                                  >  1:364373/1‑139 (MQ=255)
                                                                                                     ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTc                                                            >  1:215425/1‑111 (MQ=255)
                                                                                                     ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTc                                                            <  2:215425/111‑1 (MQ=255)
                                                                                                            aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat                         <  1:391920/139‑1 (MQ=255)
                                                                                                            aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat                         >  2:703954/1‑139 (MQ=255)
                                                                                                            aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat                         <  2:661235/139‑1 (MQ=255)
                                                                                                            aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat                         >  1:661235/1‑139 (MQ=255)
                                                                                                               ggTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgatgat                      >  2:298509/1‑139 (MQ=255)
                                                                                                                 tttGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATcc                    <  1:703954/139‑1 (MQ=255)
                                                                                                                         gACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTg            <  2:225561/139‑1 (MQ=255)
                                                                                                                               aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGaa                                                <  2:243966/97‑1 (MQ=255)
                                                                                                                               aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGaa                                                >  1:243966/1‑97 (MQ=255)
                                                                                                                               aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGc      >  2:16600/1‑139 (MQ=255)
                                                                                                                               aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGc      <  1:350747/139‑1 (MQ=255)
                                                                                                                                  aaGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAat   <  1:650848/139‑1 (MQ=255)
                                                                                                                                   aGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAata  <  2:250926/139‑1 (MQ=255)
                                                                                                                                   aGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAata  <  1:298509/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TGTCCGCAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAATAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATA  >  NC_000913/1402924‑1403193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: