Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 147,290 C→T A108V (GCT→GTT)  yadD → transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913147,2900CT100.0% 168.2 / NA 52A108V (GCT→GTT) yadDtransposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/30);  total (22/30)

TGCTCTATTCCGTGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGCTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGTCTATAA  >  NC_000913/147155‑147416
                                                                                                                                       |                                                                                                                              
tGCTCTATTCCGTGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGa                                                                                                                             >  1:9849/1‑139 (MQ=255)
         ccGTGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATaa                                                                                                                    >  1:736889/1‑139 (MQ=255)
            tGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCt                                                                                                                 >  1:581242/1‑139 (MQ=255)
                     aGGGCAATCCCGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCtggt                                                                                                        <  1:436008/139‑1 (MQ=255)
                              ccGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGTAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGAt                                                                                               <  2:104276/139‑1 (MQ=255)
                              ccGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGATGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGAt                                                                                               <  2:427296/139‑1 (MQ=255)
                              ccGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGAt                                                                                               <  2:202763/139‑1 (MQ=255)
                              ccGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGAt                                                                                               <  2:665528/139‑1 (MQ=255)
                                        gCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGc                                                                                                   >  1:587150/1‑125 (MQ=255)
                                        gCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGc                                                                                                   <  2:587150/125‑1 (MQ=255)
                                            gttgtGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGa                                                                                                                             <  1:559210/95‑1 (MQ=255)
                                            gttgtGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGACTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGa                                                                                                                             >  2:559210/1‑95 (MQ=255)
                                              tgtgATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGc                                                                               <  2:542853/139‑1 (MQ=255)
                                              tgtgATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGc                                                                               <  1:245396/139‑1 (MQ=255)
                                                    tGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGcc                                                                         <  2:217588/139‑1 (MQ=255)
                                                        cacCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCacac                                                                     >  1:83462/1‑139 (MQ=255)
                                                                 aagcCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGc                                                            <  1:69216/139‑1 (MQ=255)
                                                                    ccGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTAt                                                         <  2:221764/139‑1 (MQ=255)
                                                                        aTAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATc                                                                                    <  2:139266/108‑1 (MQ=255)
                                                                        aTAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATc                                                                                    >  1:139266/1‑108 (MQ=255)
                                                                           aGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAAtgtg                                                  <  2:18797/139‑1 (MQ=255)
                                                                                 tGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGc                                                                               >  1:524450/1‑104 (MQ=255)
                                                                                 tGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGc                                                                               <  2:524450/104‑1 (MQ=255)
                                                                                  ggCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGAt                                                                                               >  1:110197/1‑87 (MQ=255)
                                                                                  ggCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGAt                                                                                               <  2:110197/87‑1 (MQ=255)
                                                                                   gCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCtggt                                                                                                        >  2:402022/1‑77 (MQ=255)
                                                                                   gCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCtggt                                                                                                        <  1:402022/77‑1 (MQ=255)
                                                                                      tttCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGata                                       >  1:13969/1‑139 (MQ=255)
                                                                                       ttCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGAc                                                                                                                            >  2:232478/1‑53 (MQ=255)
                                                                                       ttCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGTTGAc                                                                                                                            <  1:232478/53‑1 (MQ=255)
                                                                                                        tCTATAGCCGCCATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGc                     <  2:433425/139‑1 (MQ=255)
                                                                                                              gccgccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGgcgc               >  1:237765/1‑139 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGa                                                                                                <  2:642656/55‑1 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGa                                                                                                >  1:642656/1‑55 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATc                                                               >  1:628010/1‑88 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATc                                                               <  2:628010/88‑1 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            <  1:634351/139‑1 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            <  2:247498/139‑1 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            >  1:64003/1‑139 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            >  1:247498/1‑139 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            >  2:449346/1‑139 (MQ=255)
                                                                                                                 gccATGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            <  1:725140/139‑1 (MQ=255)
                                                                                                                     tGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGt        <  1:449346/139‑1 (MQ=255)
                                                                                                                     tGCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGt        <  2:237765/139‑1 (MQ=255)
                                                                                                                      gCACCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGTc       >  1:101022/1‑139 (MQ=255)
                                                                                                                        aCCGGCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGTCta     <  2:9849/139‑1 (MQ=255)
                                                                                                                            gCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGc                                                            <  1:138423/80‑1 (MQ=255)
                                                                                                                            gCATCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGc                                                            >  2:138423/1‑80 (MQ=255)
                                                                                                                               tCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            <  2:502704/125‑1 (MQ=255)
                                                                                                                               tCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGAc            >  1:502704/1‑125 (MQ=255)
                                                                                                                               tCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGTCTATaa  >  2:657582/1‑135 (MQ=255)
                                                                                                                               tCTGGAGGTTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGTCTATaa  <  1:657582/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                              
TGCTCTATTCCGTGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATTGAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCCGCCATGCACCGGCATCTGGAGGCTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTGTTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTACTCGCCGGAGCTGGCGCGACGCGTCTATAA  >  NC_000913/147155‑147416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑