Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,947	T→C	T3A (ACT→GCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,947	0	T	C	100.0%	242.0 / NA	68	T3A (ACT→GCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

GGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCA  >  NC_000913/150813‑151066                                                                                                                                       |                                                                                                                        ggTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTc                                                                                                                     >  1:441308/1‑139 (MQ=255)   tCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAt                                                                                                                   >  1:432970/1‑139 (MQ=255)     aTCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAttt                                                                                                                 >  1:604823/1‑139 (MQ=255)     aTCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAttt                                                                                                                 >  1:157649/1‑139 (MQ=255)      tCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                >  2:421421/1‑139 (MQ=255)      tCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                <  1:221597/139‑1 (MQ=255)              gCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                        <  2:432970/139‑1 (MQ=255)                aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                      <  2:192166/139‑1 (MQ=255)                aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                      <  1:25969/139‑1 (MQ=255)                 gTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGt                                                                                                     <  2:507453/139‑1 (MQ=255)                   ggAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGttt                                                                                                   <  2:604823/139‑1 (MQ=255)                        ggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              <  2:441308/139‑1 (MQ=255)                         gCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTAt                                                                                             >  2:124362/1‑139 (MQ=255)                                       cAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                               <  1:398993/139‑1 (MQ=255)                                              tGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGtttt                                                                        >  2:256075/1‑139 (MQ=255)                                               gCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGttttt                                                                       >  1:684046/1‑139 (MQ=255)                                               gCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGttttt                                                                       <  2:157649/139‑1 (MQ=255)                                                cATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTtttg                                                                      <  2:577890/139‑1 (MQ=255)                                                     tttCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              <  2:694006/110‑1 (MQ=255)                                                     tttCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              >  1:694006/1‑110 (MQ=255)                                                         ccACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                             <  1:194405/139‑1 (MQ=255)                                                            caATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                          <  1:256075/139‑1 (MQ=255)                                                             aaTAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgta                                                         >  2:576500/1‑139 (MQ=255)                                                              aTAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtag                                                        <  2:390547/139‑1 (MQ=255)                                                                    cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                          >  1:118029/1‑131 (MQ=255)                                                                    cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                  <  1:343571/139‑1 (MQ=255)                                                                    cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTCTTTGTTTGTTATTCgt                                                          <  2:118029/131‑1 (MQ=255)                                                                      tGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATc                                                <  2:35400/139‑1 (MQ=255)                                                                       gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                               <  1:597359/107‑1 (MQ=255)                                                                       gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                               >  2:597359/1‑107 (MQ=255)                                                                        tataTGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTTCCTGACGATGTCCGTCATTGTTACTCCAATGTTTCTTTATTGGTATGGTTTGCGCTTTTTTTGTTAGTTATTCGTAGTAAATATCCa                                              <  2:715480/139‑1 (MQ=255)                                                                         atatGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCAc                                             <  1:421421/139‑1 (MQ=255)                                                                              cACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAaca                                        >  2:135625/1‑139 (MQ=255)                                                                               actgactgTATTACAACAAGAATACAGTGGTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAacac                                       >  1:536880/1‑139 (MQ=255)                                                                               actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              >  2:614990/1‑84 (MQ=255)                                                                               actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              <  1:614990/84‑1 (MQ=255)                                                                               actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAacac                                       >  2:108918/1‑139 (MQ=255)                                                                                  gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                                       >  1:238440/1‑120 (MQ=255)                                                                                  gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                                       <  2:238440/120‑1 (MQ=255)                                                                                  gactgTATTACAACAAGAATACAGTGCT‑AAAAAAGGATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              >  1:253052/1‑80 (MQ=255)                                                                                  gactgTATTACAACAAGAATACAGTGCT‑AAAAAAGGATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                              <  2:253052/80‑1 (MQ=255)                                                                                   actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                  <  2:507032/124‑1 (MQ=255)                                                                                   actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                  >  1:507032/1‑124 (MQ=255)                                                                                   actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATgg                                   >  1:670435/1‑139 (MQ=255)                                                                                    ctgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGCTAGCCATCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGc                                  >  2:509101/1‑139 (MQ=255)                                                                                      gTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTc                                <  2:684046/139‑1 (MQ=255)                                                                                                 aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                     >  1:548896/1‑139 (MQ=255)                                                                                                 aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                     >  2:615840/1‑139 (MQ=255)                                                                                                   aaTACAGTGGTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                   <  2:536880/139‑1 (MQ=255)                                                                                                   aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                   >  1:650994/1‑139 (MQ=255)                                                                                                       cAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGTTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGt               >  2:45975/1‑139 (MQ=255)                                                                                                        aGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTa              <  1:615840/139‑1 (MQ=255)                                                                                                         gTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTaa             >  1:121563/1‑139 (MQ=255)                                                                                                          tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                                       >  2:524995/1‑96 (MQ=255)                                                                                                          tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                                       <  1:524995/96‑1 (MQ=255)                                                                                                          tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                      >  2:3099/1‑113 (MQ=255)                                                                                                          tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                      <  1:3099/113‑1 (MQ=255)                                                                                                          tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAac            >  1:388143/1‑139 (MQ=255)                                                                                                            cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                             <  2:425002/88‑1 (MQ=255)                                                                                                            cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                             >  1:425002/1‑88 (MQ=255)                                                                                                            cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                      <  2:437845/111‑1 (MQ=255)                                                                                                            cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                      >  1:437845/1‑111 (MQ=255)                                                                                                            cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCAt                              <  1:704182/119‑1 (MQ=255)                                                                                                            cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCAt                              >  2:704182/1‑119 (MQ=255)                                                                                                             tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                              >  1:95703/1‑86 (MQ=255)                                                                                                             tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                              <  2:95703/86‑1 (MQ=255)                                                                                                             tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt         <  2:473848/139‑1 (MQ=255)                                                                                                                    aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCa  >  1:152043/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                        GGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCA  >  NC_000913/150813‑151066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑