Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,462,463	G→A	W113* (TGG→TGA)	paaK →	phenylacetyl‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,462,463	0	G	A	100.0%	102.3 / NA	34	W113* (TGG→TGA)	paaK	phenylacetyl‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

GATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGGGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTGGGCGCTACGG  >  NC_000913/1462332‑1462596                                                                                                                                    |                                                                                                                                      gATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGc                                                                                                                                     <  2:123693/134‑1 (MQ=255)   tCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATAtt                                                                                                                              >  1:275632/1‑139 (MQ=255)       cgcgACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAg                                                                                                                          >  1:326566/1‑139 (MQ=255)         cgACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAgcg                                                                                                                        <  2:484476/139‑1 (MQ=255)                 aTCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCttt                                                                                                                >  2:320849/1‑139 (MQ=255)                       ttGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGc                                                                                                                                     >  1:283175/1‑112 (MQ=255)                       ttGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGc                                                                                                                                     <  2:283175/112‑1 (MQ=255)                       ttGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTtgcgtgc                                                                                                          >  2:538075/1‑139 (MQ=255)                                         cAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCaa                                                                                        <  1:320849/139‑1 (MQ=255)                                                        tgCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGt                                                                         >  1:191447/1‑139 (MQ=255)                                                                        ttcGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGc                                                         <  1:538075/139‑1 (MQ=255)                                                                        ttcGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGc                                                         <  2:275632/139‑1 (MQ=255)                                                                             gAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGtt                                                    <  1:383187/139‑1 (MQ=255)                                                                               aCCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTa                                                  >  1:217604/1‑139 (MQ=255)                                                                                 cacaGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACt                                                >  1:641541/1‑139 (MQ=255)                                                                                    aGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCa                                                                                                      >  2:663421/1‑82 (MQ=255)                                                                                    aGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCa                                                                                                      <  1:663421/82‑1 (MQ=255)                                                                                       tAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGc                                                         <  2:636346/124‑1 (MQ=255)                                                                                       tAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGc                                                         >  1:636346/1‑124 (MQ=255)                                                                                           ccGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCt                                                                                               <  2:610649/82‑1 (MQ=255)                                                                                           ccGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCt                                                                                               >  1:610649/1‑82 (MQ=255)                                                                                           ccGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTgg                                      >  1:393875/1‑139 (MQ=255)                                                                                            cGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTggg                                     <  2:496501/139‑1 (MQ=255)                                                                                              aCAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGc                                                         <  1:568825/117‑1 (MQ=255)                                                                                              aCAGTTGTCGGCTATACGCAAAACGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGc                                                         >  2:568825/1‑117 (MQ=255)                                                                                                                   aaCGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAGATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTc              >  2:442179/1‑139 (MQ=255)                                                                                                                   aaCGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACt                                                <  2:302811/105‑1 (MQ=255)                                                                                                                   aaCGATATTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACt                                                >  1:302811/1‑105 (MQ=255)                                                                                                                       atatTGATAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTggg          <  2:191447/139‑1 (MQ=255)                                                                                                                             aTAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGc                                         >  1:132497/1‑102 (MQ=255)                                                                                                                             aTAACTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGc                                         <  2:132497/102‑1 (MQ=255)                                                                                                                             aTAACTGAGCCAATATTGTAGCGCGTTCTTTGCATGCGGCAGGGGGCTCGCCCAAAGACAAAAGTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCAATATGGTGCCGAACGTCTGGGCGCtgc    >  2:270497/1‑137 (MQ=255)                                                                                                                               aaCTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTGGGCGCTACgg  >  2:24888/1‑139 (MQ=255)                                                                                                                               aaCTGAGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTGGGCGCTACgg  >  2:643409/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      GATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCAAAACGATATTGATAACTGGGCCAATATTGTAGCGCGTTCTTTGCGTGCGGCAGGGGGCTCGCCCAAAGACAAAATTCATGTTGCCTATGGTTACGGCCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTGGGCGCTACGG  >  NC_000913/1462332‑1462596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑