Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,521,048	A→G	V673A (GTC→GCC)	yncD ←	putative iron outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,521,048	0	A	G	100.0%	167.0 / NA	47	V673A (GTC→GCC)	yncD	putative iron outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/24);  total (23/24)

GCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCC  >  NC_000913/1520912‑1521179                                                                                                                                         |                                                                                                                                    gCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCa                                                                                                                                   >  1:487008/1‑139 (MQ=255)     tACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGa                                                                                                                               >  1:154319/1‑139 (MQ=255)        aaTCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAg                                                                                                                            <  2:291196/139‑1 (MQ=255)            gTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAAcc                                                                                                                        >  1:101497/1‑139 (MQ=255)            gTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAAcc                                                                                                                        >  1:224334/1‑139 (MQ=255)                                       ggCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCa                                                                                                                                   >  1:214614/1‑101 (MQ=255)                                       ggCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCa                                                                                                                                   <  2:214614/101‑1 (MQ=255)                                       ggCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATc                                                                                             >  2:389629/1‑139 (MQ=255)                                               ggTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:767025/139‑1 (MQ=255)                                                         cAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           <  2:643200/139‑1 (MQ=255)                                                         cAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           >  2:602418/1‑139 (MQ=255)                                                          aaaTCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     >  2:72557/1‑128 (MQ=255)                                                          aaaTCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:72557/128‑1 (MQ=255)                                                               tcCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGt                                                                     <  1:171896/139‑1 (MQ=255)                                                                 cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           >  2:660362/1‑131 (MQ=255)                                                                 cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           <  1:660362/131‑1 (MQ=255)                                                                 cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTa                                                                   >  2:590639/1‑139 (MQ=255)                                                                        tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATtgtg                                                            <  2:101497/139‑1 (MQ=255)                                                                        tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATtgtg                                                            <  2:224334/139‑1 (MQ=255)                                                                        tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATtgtg                                                            >  1:311964/1‑139 (MQ=255)                                                                              tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         >  1:716985/1‑120 (MQ=255)                                                                              tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         <  2:716985/120‑1 (MQ=255)                                                                              tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAtt                                                      <  1:445032/139‑1 (MQ=255)                                                                                ccGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:512847/106‑1 (MQ=255)                                                                                ccGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     >  2:512847/1‑106 (MQ=255)                                                                                    cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                     <  1:220120/70‑1 (MQ=255)                                                                                    cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                     >  2:220120/1‑70 (MQ=255)                                                                                       cATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTtata                                             <  2:311964/139‑1 (MQ=255)                                                                                               cGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:391080/91‑1 (MQ=255)                                                                                               cGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     >  2:391080/1‑91 (MQ=255)                                                                                                    gggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCACTGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAg                                <  2:487008/139‑1 (MQ=255)                                                                                                     ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                          <  1:339047/48‑1 (MQ=255)                                                                                                     ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                          >  2:339047/1‑48 (MQ=255)                                                                                                         aaGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgac                           <  1:389629/139‑1 (MQ=255)                                                                                                                gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAAt                                                                                                     >  2:536741/1‑58 (MQ=255)                                                                                                                gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAAt                                                                                                     <  1:536741/58‑1 (MQ=255)                                                                                                                gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         >  2:653056/1‑86 (MQ=255)                                                                                                                gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         <  1:653056/86‑1 (MQ=255)                                                                                                                      tCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGAc              >  2:754118/1‑139 (MQ=255)                                                                                                                         cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGgc           >  1:10255/1‑139 (MQ=255)                                                                                                                          ccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCG‑ATAATTTAt                                                                                              <  2:335259/54‑1 (MQ=255)                                                                                                                           cGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGgcgc         >  2:720661/1‑139 (MQ=255)                                                                                                                            gTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATTGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTg                                                     <  1:461333/94‑1 (MQ=255)                                                                                                                            gTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTg                                                     >  2:461333/1‑94 (MQ=255)                                                                                                                              ttGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                       <  2:388688/90‑1 (MQ=255)                                                                                                                              ttGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                       >  1:388688/1‑90 (MQ=255)                                                                                                                                  cTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGcc  <  1:720661/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCC  >  NC_000913/1520912‑1521179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑