| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,586,436 | A→G | A17A (GCT→GCC) | ydeP ← | putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,586,436 | 0 | A | G | 98.6% | 258.3 / NA | 73 | A17A (GCT→GCC) | ydeP | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (34/38); minor base T (0/1); total (34/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGG > NC_000913/1586304‑1586571 | cGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCAccc < 2:740324/139‑1 (MQ=255) tgCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAaccac > 2:577541/1‑139 (MQ=255) ttAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTg < 1:355926/139‑1 (MQ=255) aGGATCTGGCCATGCACAACCCGGACAGTCAAAGTCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTgca < 1:600430/139‑1 (MQ=255) aTCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTGCTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTgcagca < 2:544692/139‑1 (MQ=255) tGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAg > 1:603838/1‑139 (MQ=255) aCCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCa > 2:152713/1‑139 (MQ=255) ggACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAttt > 1:311217/1‑139 (MQ=255) gACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAtttt > 2:63206/1‑139 (MQ=255) gTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTttc > 1:643868/1‑139 (MQ=255) gTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTttc > 1:216737/1‑139 (MQ=255) aaGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCa < 1:317700/139‑1 (MQ=255) aaGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCa < 2:603838/139‑1 (MQ=255) aaGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCa < 2:598665/139‑1 (MQ=255) aaGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACAGCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCa < 1:627455/139‑1 (MQ=255) aaGCCCTCTCGCTTATTCATGTCAAACATGCCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCCCTACGGATTTAACTGCCCCCCAACCTCCTGCAGCACTCTGGTAGGATTCAATTTTTTTCTTCa < 2:115016/139‑1 (MQ=255) ctctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTAc > 1:5803/1‑139 (MQ=255) ctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCa < 1:401605/132‑1 (MQ=255) ctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCa > 2:401605/1‑132 (MQ=255) ctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACtt > 1:605896/1‑139 (MQ=255) gCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATc < 2:582184/139‑1 (MQ=255) aTTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCtgt > 1:722824/1‑139 (MQ=255) ttCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCa < 2:314393/83‑1 (MQ=255) ttCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCa > 1:314393/1‑83 (MQ=255) gTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTgca < 1:566090/88‑1 (MQ=255) gTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTgca > 2:566090/1‑88 (MQ=255) gTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGaa > 1:334032/1‑139 (MQ=255) gTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGaa > 2:50533/1‑139 (MQ=255) gCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAattatt > 2:304423/1‑139 (MQ=255) gCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAattatt > 1:727290/1‑139 (MQ=255) cAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAAttatta < 1:152713/139‑1 (MQ=255) tAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGa > 1:650896/1‑139 (MQ=255) tCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGt > 2:109352/1‑139 (MQ=255) ccTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTg < 1:508341/66‑1 (MQ=255) ccTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTAAGGATTTAACGGCACTCCAACCACCTg > 2:508341/1‑66 (MQ=255) tGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAAt < 1:433595/139‑1 (MQ=255) gCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAg < 2:216737/139‑1 (MQ=255) cGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGc > 2:603894/1‑139 (MQ=255) atatCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCaa > 1:119007/1‑51 (MQ=255) atatCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCaa < 2:119007/51‑1 (MQ=255) tatCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCgatg > 1:207624/1‑139 (MQ=255) cATCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGc < 1:577541/139‑1 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAgg < 2:449522/118‑1 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAgg > 1:449522/1‑118 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca > 1:9536/1‑139 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca < 1:109352/139‑1 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca < 2:5803/139‑1 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca < 2:600988/139‑1 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca < 2:311217/139‑1 (MQ=255) aTCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca < 1:63206/139‑1 (MQ=255) tCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac < 2:605896/139‑1 (MQ=255) tCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac < 2:643868/139‑1 (MQ=255) tCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac < 2:650896/139‑1 (MQ=255) tCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac < 1:285152/139‑1 (MQ=255) tCTGCTTACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac < 1:50533/139‑1 (MQ=255) ttACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGtac > 1:630995/1‑139 (MQ=255) tACGTACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGtaca > 2:745776/1‑139 (MQ=255) tACTGCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGtacataca < 1:12587/139‑1 (MQ=255) gCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCtgtg > 2:406610/1‑85 (MQ=255) gCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCtgtg < 1:406610/85‑1 (MQ=255) gCATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAgg > 2:312427/1‑139 (MQ=255) cATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac < 2:375839/124‑1 (MQ=255) cATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGcac > 1:375839/1‑124 (MQ=255) cATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca > 2:110999/1‑123 (MQ=255) cATTCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGca < 1:110999/123‑1 (MQ=255) ttCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCGTGTGTGGAAATGAATTATTAGGACTTGTAATAGCAATGATGCACAGTACATACAAAGGGAt > 2:491569/1‑139 (MQ=255) ttCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAAttatta > 2:430550/1‑98 (MQ=255) ttCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAAttatta < 1:430550/98‑1 (MQ=255) tCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTgg < 2:405321/44‑1 (MQ=255) tCGCTACGGATTTAACGGCACCCCAACCACCTGCAGCACCCTgg > 1:405321/1‑44 (MQ=255) aaCGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATgg < 2:334032/139‑1 (MQ=255) aaCGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATgg < 1:304423/139‑1 (MQ=255) aaCGGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACCTAACCTTTTTTGAAATGAATTAATAGGATTTGTAATAGCGATGATGGACAGTACATAAAAAGGGATTATCGCCAACATgg < 2:722824/139‑1 (MQ=255) | CGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGG > NC_000913/1586304‑1586571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |