Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,218	G→A	*397* (TAG→TAA)	ydeA →	arabinose efflux transporter, arabinose‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,218	0	G	A	100.0%	108.4 / NA	35	*397* (TAG→TAA)	ydeA	arabinose efflux transporter, arabinose‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

AGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGA  >  NC_000913/1618091‑1618324                                                                                                                                |                                                                                                           aGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGccc                                                                                                 >  2:72045/1‑139 (MQ=255)                       gTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                           <  2:161030/139‑1 (MQ=255)                           atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGtt                                                                       >  2:553382/1‑139 (MQ=255)                              atTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTa                                                                    <  1:380012/139‑1 (MQ=255)                                          ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                        >  2:253/1‑139 (MQ=255)                                            cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                            >  2:571500/1‑117 (MQ=255)                                            cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                            <  1:571500/117‑1 (MQ=255)                                             gcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                     <  1:553382/139‑1 (MQ=255)                                                 tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                 <  1:56776/139‑1 (MQ=255)                                                       cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataa                                           >  2:233087/1‑139 (MQ=255)                                                              cgcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                    <  1:253/139‑1 (MQ=255)                                                                cgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                  <  2:750484/139‑1 (MQ=255)                                                                   tAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                               <  2:327725/139‑1 (MQ=255)                                                                          gTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACGGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                    >  1:39854/1‑127 (MQ=255)                                                                          gTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACGGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                    <  2:39854/127‑1 (MQ=255)                                                                              aTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATAcc                    <  1:406845/139‑1 (MQ=255)                                                                                   tataTTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGAt                                                      <  2:128659/100‑1 (MQ=255)                                                                                   tataTTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGAt                                                      >  1:128659/1‑100 (MQ=255)                                                                                     tatTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGa             <  1:72045/139‑1 (MQ=255)                                                                                         tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGc                                                                                                   <  1:410161/49‑1 (MQ=255)                                                                                         tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGc                                                                                                   >  2:410161/1‑49 (MQ=255)                                                                                         tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                   >  2:762483/1‑113 (MQ=255)                                                                                         tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                   <  1:762483/113‑1 (MQ=255)                                                                                         tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAAt           >  1:643732/1‑137 (MQ=255)                                                                                         tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAAt           <  2:643732/137‑1 (MQ=255)                                                                                          cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcac                                 <  2:174335/114‑1 (MQ=255)                                                                                          cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcac                                 >  1:174335/1‑114 (MQ=255)                                                                                          cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                  >  2:501996/1‑113 (MQ=255)                                                                                          cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                  <  1:501996/113‑1 (MQ=255)                                                                                           gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa       <  1:75881/139‑1 (MQ=255)                                                                                           gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa       >  2:712793/1‑139 (MQ=255)                                                                                               cTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt      <  2:188192/136‑1 (MQ=255)                                                                                               cTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt      >  1:188192/1‑136 (MQ=255)                                                                                                                     cAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGa  >  1:649974/1‑118 (MQ=255)                                                                                                                     cAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGa  <  2:649974/118‑1 (MQ=255)                                                                                                                                |                                                                                                           AGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGA  >  NC_000913/1618091‑1618324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑