Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,672,639	T→C	L227P (CTG→CCG)	ydgD →	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,672,639	0	T	C	100.0%	79.1 / NA	23	L227P (CTG→CCG)	ydgD	putative peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/8);  total (15/8)

GGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAA  >  NC_000913/1672511‑1672756                                                                                                                                 |                                                                                                                      ggcaggcTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAACACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCAt                                                                                                             >  1:653651/1‑139 (MQ=255)            cTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACgg                                                                                                  <  2:468100/139‑1 (MQ=255)            cTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACgg                                                                                                  <  1:143037/139‑1 (MQ=255)                aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                              <  1:30452/139‑1 (MQ=255)                aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                              >  1:432040/1‑139 (MQ=255)                aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                              >  2:30452/1‑139 (MQ=255)                                          atcaAAACTGTGAAGTGACTTGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCgtc                                                                    >  2:453217/1‑137 (MQ=255)                                             aaaaCTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTcc                                                                 >  1:300660/1‑139 (MQ=255)                                                     gAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGa                                                         >  2:763623/1‑139 (MQ=255)                                                                  gggCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgcg                                            <  2:432040/139‑1 (MQ=255)                                                                     cgcAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTg                                                               <  1:189951/117‑1 (MQ=255)                                                                     cgcAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTg                                                               >  2:189951/1‑117 (MQ=255)                                                                                      tCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCAttt                        >  1:9647/1‑139 (MQ=255)                                                                                           tCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGtt                   >  2:731340/1‑139 (MQ=255)                                                                                              gTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTAcc                >  1:634328/1‑139 (MQ=255)                                                                                                   aTACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAAc                                  >  1:715902/1‑116 (MQ=255)                                                                                                   aTACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAAc                                  <  2:715902/116‑1 (MQ=255)                                                                                                         tggcGGGTGACCGCGGTTCGCCTCCGATGTTGCATACCGATGACGCCTGGCATTTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGa     >  2:446705/4‑139 (MQ=255)                                                                                                         tGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGa     >  2:571457/1‑139 (MQ=255)                                                                                                                   cAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACaa  <  2:170465/132‑1 (MQ=255)                                                                                                                   cAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACaa  >  1:170465/1‑132 (MQ=255)                                                                                                                           cGCCTCCGATGTTGGATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCggg                              >  1:467929/1‑96 (MQ=255)                                                                                                                           cGCCTCCGATGTTGCAGACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCggg                              <  2:467929/96‑1 (MQ=255)                                                                                                                                 |                                                                                                                      GGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAA  >  NC_000913/1672511‑1672756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑