Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,874,798 G→A *149* (TAG→TAA
T260I (ACT→ATT) 
yeaL →
yeaM ←
UPF0756 family putative inner membrane protein
putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,874,7980GA100.0% 74.8 / NA 24*149* (TAG→TAA
T260I (ACT→ATT) 
yeaL
yeaM
UPF0756 family putative inner membrane protein
putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/16);  total (8/16)

ATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGC  >  NC_000913/1874667‑1874912
                                                                                                                                   |                                                                                                                  
aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                             <  2:138454/139‑1 (MQ=255)
    gCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                                         <  2:202886/139‑1 (MQ=255)
        ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAatat                                                                                                     >  2:736392/1‑139 (MQ=255)
              gCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGc                                                                                               <  1:338876/139‑1 (MQ=255)
               cAACTGGTCGCCGGGCTGCTTGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGcc                                                                                              <  2:441889/139‑1 (MQ=255)
                                          aCTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                   <  1:736392/139‑1 (MQ=255)
                                           cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                                        <  2:64269/117‑1 (MQ=255)
                                           cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                                        >  1:64269/1‑117 (MQ=255)
                                                        aGCGCTGTTTCGCGGCATACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATACATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                   <  2:162875/125‑1 (MQ=255)
                                                        aGCGCTGTTTCGCGGCATACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATACATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                   >  1:162875/1‑125 (MQ=255)
                                                           gcTGTTTCGTGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCg                                                  <  1:326712/139‑1 (MQ=255)
                                                           gcTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCg                                                  <  2:376816/139‑1 (MQ=255)
                                                                         tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAc                                    >  2:406272/1‑139 (MQ=255)
                                                                           ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAccc                                  <  2:564129/139‑1 (MQ=255)
                                                                                    ccGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                                          <  1:714128/122‑1 (MQ=255)
                                                                                    ccGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                                          >  2:714128/1‑122 (MQ=255)
                                                                                        ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                     <  1:556000/139‑1 (MQ=255)
                                                                                                           ttCGCTGACTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACAGAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGc  <  1:457245/139‑1 (MQ=255)
                                                                                                              gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                   <  2:384583/71‑1 (MQ=255)
                                                                                                              gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                   >  1:384583/1‑71 (MQ=255)
                                                                                                              gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc                   >  1:445028/1‑119 (MQ=255)
                                                                                                              gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc                   <  2:445028/119‑1 (MQ=255)
                                                                                                                              cAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCa                                                                                <  1:5441/42‑1 (MQ=255)
                                                                                                                              cAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCa                                                                                >  2:5441/1‑42 (MQ=255)
                                                                                                                                   |                                                                                                                  
ATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGC  >  NC_000913/1874667‑1874912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: