Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,888,695	T→C	Q351R (CAG→CGG)	fadD ←	acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,888,695	0	T	C	100.0%	161.4 / NA	45	Q351R (CAG→CGG)	fadD	acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

ATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAG  >  NC_000913/1888559‑1888823                                                                                                                                         |                                                                                                                                 atcatcatcatcCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGt                                                                                                                                >  1:142964/1‑139 (MQ=255)       atcatcCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTc                                                                                                                          >  2:223176/1‑139 (MQ=255)            ccaccaGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGttt                                                                                                                     >  1:171672/1‑139 (MQ=255)                   tttGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCaa                                                                                                              <  2:287929/139‑1 (MQ=255)                   tttGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCaa                                                                                                              >  2:243478/1‑139 (MQ=255)                       gCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGct                                                                                                          <  1:682770/139‑1 (MQ=255)                               cGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGccacc                                                                                                  <  1:223176/139‑1 (MQ=255)                                                  aTGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGTCTTCCCGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTTCCACCACTTGCTGACCTGGCATcc                                                                               >  2:225496/1‑139 (MQ=255)                                                              tGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCgg                                                                   >  2:147411/1‑139 (MQ=255)                                                                aTAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGaa                                                                 >  2:387968/1‑139 (MQ=255)                                                                aTAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGaa                                                                 <  1:243478/139‑1 (MQ=255)                                                                  aaTCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAg                                                               <  1:147411/139‑1 (MQ=255)                                                                   aTCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCAAAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGa                                                              >  2:446269/1‑139 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTg                                                                                     >  2:116197/1‑111 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTg                                                                                     <  1:116197/111‑1 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCg                                                                    >  1:276242/1‑128 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCg                                                                    <  2:276242/128‑1 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAg                                                         >  2:97936/1‑139 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAg                                                         >  1:698375/1‑139 (MQ=255)                                                                        tatCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAg                                                         >  2:542049/1‑139 (MQ=255)                                                                             tatGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCAc                                                                            >  1:657587/1‑115 (MQ=255)                                                                             tatGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCAc                                                                            <  2:657587/115‑1 (MQ=255)                                                                                  gctAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGCTACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAaaaa                                               >  2:194170/3‑139 (MQ=255)                                                                                  gTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGaaa                                               >  2:450338/1‑139 (MQ=255)                                                                                    tAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATc                                             >  2:687828/1‑139 (MQ=255)                                                                                     aaCGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGctct                                                                                                        >  1:465403/1‑79 (MQ=255)                                                                                     aaCGCTGACCAGAGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGctct                                                                                                        <  2:465403/79‑1 (MQ=255)                                                                                      aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACt                                                                                               <  1:764920/87‑1 (MQ=255)                                                                                      aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACt                                                                                               >  2:764920/1‑87 (MQ=255)                                                                                      aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGc                                                                                   <  1:692487/99‑1 (MQ=255)                                                                                      aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGc                                                                                   >  2:692487/1‑99 (MQ=255)                                                                                      aCGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCa                                           >  2:325091/1‑139 (MQ=255)                                                                                            aCCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCAc                                                                            >  2:639580/1‑100 (MQ=255)                                                                                            aCCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCAc                                                                            <  1:639580/100‑1 (MQ=255)                                                                                             ccAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGctgctg                                    <  1:344175/139‑1 (MQ=255)                                                                                                    cgcACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTc                                                                                                                               <  2:310109/41‑1 (MQ=255)                                                                                                    cgcACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTc                                                                                                                               >  1:310109/1‑41 (MQ=255)                                                                                                    cgcACACTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAActct                             >  1:229203/1‑139 (MQ=255)                                                                                                      cacaCTCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCttt                           >  1:576410/1‑139 (MQ=255)                                                                                                           tCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCAc                                                                                       >  2:345621/1‑74 (MQ=255)                                                                                                           tCGGTAAGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCAc                                                                                       <  1:345621/74‑1 (MQ=255)                                                                                                                 aGGCCATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCa                <  1:387968/139‑1 (MQ=255)                                                                                                                     cATAGCCTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGc            <  2:576410/139‑1 (MQ=255)                                                                                                                           cTTCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGaa      <  2:171672/139‑1 (MQ=255)                                                                                                                             tCCAGCAGATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACa    <  1:225496/139‑1 (MQ=255)                                                                                                                               cagcagATACCGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAg  <  1:450338/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 ATCATCATCATCCACCAGTTTGGCTTCCGTCGACGGCACCGGCAAACCGATGCTACCACTATGATAATCAATATCATATGGGTTAACGCTGACCAGCGGCGCACACTCGGTAAGGCCATAGCCTTCCAGCAGATACTGTCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAG  >  NC_000913/1888559‑1888823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑