Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,939,673 T→C N174S (AAC→AGC)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,939,6730TC100.0% 156.4 / NA 44N174S (AAC→AGC) lpxMmyristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

CATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTT  >  NC_000913/1939557‑1939800
                                                                                                                    |                                                                                                                               
cATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCgg                                                                                                           >  1:209723/1‑139 (MQ=255)
 aTGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                          <  1:503168/139‑1 (MQ=255)
      ccTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                     <  2:529224/139‑1 (MQ=255)
      ccTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                     <  2:529633/139‑1 (MQ=255)
        tGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                                   >  1:103709/1‑139 (MQ=255)
              ggTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTAt                                                                                             >  1:705095/1‑139 (MQ=255)
               gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATg                                                                                            >  2:292043/1‑139 (MQ=255)
                    taataTCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACa                                                                                       >  2:578914/1‑139 (MQ=255)
                     aataTCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACAt                                                                                      <  1:153486/139‑1 (MQ=255)
                         tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                                  >  2:397297/1‑139 (MQ=255)
                         tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                                  >  1:258660/1‑139 (MQ=255)
                         tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                                  >  1:700468/1‑139 (MQ=255)
                            ccAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGcc                                                                               >  1:398651/1‑139 (MQ=255)
                              aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAt                                                                             >  2:755159/1‑139 (MQ=255)
                                tACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAAcc                                                                                                             <  1:306458/105‑1 (MQ=255)
                                tACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAAcc                                                                                                             >  2:306458/1‑105 (MQ=255)
                                 aCCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAtttt                                                                          <  1:397297/139‑1 (MQ=255)
                                       gACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCaaaaa                                                                                                               >  1:77442/1‑96 (MQ=255)
                                       gACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCaaaaa                                                                                                               <  2:77442/96‑1 (MQ=255)
                                             ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAtttt                                                                          >  1:583547/1‑127 (MQ=255)
                                             ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAtttt                                                                          <  2:583547/127‑1 (MQ=255)
                                                aCTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                                      <  1:290465/96‑1 (MQ=255)
                                                aCTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                                      >  2:290465/1‑96 (MQ=255)
                                                             tttAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCa                                              <  1:755159/139‑1 (MQ=255)
                                                               tAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAgg                                            >  1:58045/1‑139 (MQ=255)
                                                                     cGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATc                                      >  1:619252/1‑139 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                          <  2:465428/69‑1 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                          >  1:465428/1‑69 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                     <  1:517953/74‑1 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                     >  2:517953/1‑74 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGcc                                                          >  2:582283/1‑117 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGcc                                                          <  1:582283/117‑1 (MQ=255)
                                                                       tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                    >  1:512034/1‑139 (MQ=255)
                                                                       tCATTTCCACCATGCAGACGTCCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAGCCTGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                    <  1:427094/139‑1 (MQ=255)
                                                                               cGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCaa                            <  1:578914/139‑1 (MQ=255)
                                                                                       gacgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCgg                    <  2:619252/139‑1 (MQ=255)
                                                                                        acgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGc                   <  1:292043/139‑1 (MQ=255)
                                                                                             cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAg              <  2:103709/139‑1 (MQ=255)
                                                                                             cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAg              <  2:659317/139‑1 (MQ=255)
                                                                                               ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGaa            <  2:705095/139‑1 (MQ=255)
                                                                                               ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGaa            >  1:453956/1‑139 (MQ=255)
                                                                                                   aGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGAt        <  2:58045/139‑1 (MQ=255)
                                                                                                       acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACt    <  2:700468/139‑1 (MQ=255)
                                                                                                         gacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACttt  <  2:453956/139‑1 (MQ=255)
                                                                                                                    |                                                                                                                               
CATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTT  >  NC_000913/1939557‑1939800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: