Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,952,661	G→A	*132* (TAG→TAA)	yecN →	MAPEG family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,952,661	0	G	A	100.0%	110.3 / NA	35	*132* (TAG→TAA)	yecN	MAPEG family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

TTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCC  >  NC_000913/1952540‑1952787                                                                                                                          |                                                                                                                               ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCAc                                                                                                                 <  2:373231/137‑1 (MQ=255) ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCAc                                                                                                                 >  1:373231/1‑137 (MQ=255)   catcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCActttc                                                                                                             >  1:240588/1‑139 (MQ=255)           tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTccc                                                                                                     >  1:249848/1‑139 (MQ=255)           tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGACACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTccc                                                                                                     >  2:458130/1‑139 (MQ=255)               ttCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGAt                                                                                                 >  1:722668/1‑139 (MQ=255)                tCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGATATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGAtt                                                                                                <  1:286494/139‑1 (MQ=255)                  cGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTTCGCTGTTGCTGATGGTGCTGGCGAATTTTTTGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGAtttt                                                                                              <  2:554618/139‑1 (MQ=255)                              tCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTATTCCCGGATTTTTACGGTATGtct                                                                                  <  1:471422/139‑1 (MQ=255)                                     tGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcgcg                                                                           <  1:633792/139‑1 (MQ=255)                                       aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                         <  1:89098/139‑1 (MQ=255)                                                   tgtgCGCTATTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACGCGCTATTTTCt                                                             >  1:500606/1‑139 (MQ=255)                                                    gtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                            <  1:359087/139‑1 (MQ=255)                                                    gtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                            <  2:249848/139‑1 (MQ=255)                                                       cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                         >  1:437723/1‑139 (MQ=255)                                                        gcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                        <  2:499259/139‑1 (MQ=255)                                                        gcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                        >  1:492052/1‑139 (MQ=255)                                                        gcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGATTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                        <  1:488274/139‑1 (MQ=255)                                                         cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCt                                                       <  1:587965/139‑1 (MQ=255)                                                                      tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                        >  1:466058/1‑93 (MQ=255)                                                                      tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                        <  2:466058/93‑1 (MQ=255)                                                                             gAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                   <  1:409908/139‑1 (MQ=255)                                                                                tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAc                                <  1:667432/139‑1 (MQ=255)                                                                                          atGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                      >  1:147573/1‑139 (MQ=255)                                                                                           tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAAc                     >  2:12270/1‑139 (MQ=255)                                                                                            gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                          <  1:616003/69‑1 (MQ=255)                                                                                            gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                          >  2:616003/1‑69 (MQ=255)                                                                                              ccTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                  >  2:707282/1‑139 (MQ=255)                                                                                                     gTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                    >  2:31426/1‑82 (MQ=255)                                                                                                     gTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                    <  1:31426/82‑1 (MQ=255)                                                                                                        ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt        >  1:474648/1‑139 (MQ=255)                                                                                                         gTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc       <  2:240588/139‑1 (MQ=255)                                                                                                         gTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCATTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc       <  2:492052/139‑1 (MQ=255)                                                                                                              ctcCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc  <  1:350692/139‑1 (MQ=255)                                                                                                              ctcCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc  <  2:84055/139‑1 (MQ=255)                                                                                                                          |                                                                                                                               TTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCC  >  NC_000913/1952540‑1952787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑