Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,073,094	T→C	L519P (CTG→CCG)	flu →	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,073,094	0	T	C	100.0%	277.8 / NA	79	L519P (CTG→CCG)	flu	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/38);  total (41/38)

ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGA  >  NC_000913/2072958‑2073228                                                                                                                                         |                                                                                                                                       aTCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCCGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                      <  2:160795/139‑1 (MQ=255)  tCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgac                                                                                                                                     >  2:205175/1‑139 (MQ=255)   ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                    >  1:100576/1‑139 (MQ=255)   ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                    >  1:508853/1‑139 (MQ=255)   ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                    >  2:604248/1‑139 (MQ=255)   ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACCGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                    >  2:109027/1‑139 (MQ=255)    cGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgc                                                                                                                                   <  1:391036/139‑1 (MQ=255)    cGTGAAGGCGATGCAATCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgc                                                                                                                                   <  2:336612/139‑1 (MQ=255)     gTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgct                                                                                                                                  >  1:483104/1‑139 (MQ=255)      tGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 <  2:483104/139‑1 (MQ=255)      tGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 <  1:604248/139‑1 (MQ=255)        aaGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                               <  2:481602/139‑1 (MQ=255)           gCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGa                                                                                                                            <  1:205175/139‑1 (MQ=255)             gATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                      >  2:190864/1‑127 (MQ=255)             gATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                      <  1:190864/127‑1 (MQ=255)              aTGCACTCTTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGCCAg                                                                                                                         >  1:500490/1‑139 (MQ=255)              aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAg                                                                                                                         >  2:542392/1‑139 (MQ=255)                      cTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAATACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTca                                                                                                                 <  2:450564/139‑1 (MQ=255)                       tGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcac                                                                                                                >  1:261136/1‑139 (MQ=255)                        gCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                               <  2:117658/139‑1 (MQ=255)                           gggAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACg                                                                                                            >  1:58453/1‑139 (MQ=255)                              aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                         >  2:350779/1‑139 (MQ=255)                              aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGACGTCAACCTGAATGCAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                         >  2:405575/1‑139 (MQ=255)                                gCTCTCTCACCGGTAACGGCAGTGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                       >  1:387093/1‑139 (MQ=255)                                gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                       >  2:678931/1‑139 (MQ=255)                                gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                       >  2:194933/1‑139 (MQ=255)                                 ctctctCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTc                                                                                                      >  2:39384/1‑139 (MQ=255)                                  tctctcACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCa                                                                                                     <  1:39384/139‑1 (MQ=255)                                      tcACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGc                                                                                                 >  2:580777/1‑139 (MQ=255)                                            gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCTCGGATGTCATTGCTCAgcg                                                                                           >  2:376855/1‑139 (MQ=255)                                            gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  2:20096/139‑1 (MQ=255)                                            gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  1:635558/139‑1 (MQ=255)                                            gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  1:289886/139‑1 (MQ=255)                                            gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  2:164823/139‑1 (MQ=255)                                            gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  2:228119/139‑1 (MQ=255)                                               aCGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 >  2:304387/1‑98 (MQ=255)                                               aCGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                 <  1:304387/98‑1 (MQ=255)                                               aCGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCgg                                                                                        >  2:171941/1‑139 (MQ=255)                                                  gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                                     >  2:583145/1‑139 (MQ=255)                                                     gCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGc                                                                                  >  1:388981/1‑139 (MQ=255)                                                      cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                                                 >  2:402396/1‑139 (MQ=255)                                                               aTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCGAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                        <  2:500490/139‑1 (MQ=255)                                                               aTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                        <  2:261136/139‑1 (MQ=255)                                                                  aGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGAcc                                                                     >  2:47441/1‑139 (MQ=255)                                                                         ggCATACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:434572/139‑1 (MQ=255)                                                                         ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:283945/139‑1 (MQ=255)                                                                         ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:583145/139‑1 (MQ=255)                                                                         ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  1:47441/139‑1 (MQ=255)                                                                         ggCACACCCACTGTCAGCAACACCCCACCCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  2:549817/139‑1 (MQ=255)                                                                                tcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCt                                                       >  1:161933/1‑139 (MQ=255)                                                                                  actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGa                                                     >  2:358684/1‑139 (MQ=255)                                                                                    tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAAc                                                   >  1:227506/1‑139 (MQ=255)                                                                                        aGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  2:445750/95‑1 (MQ=255)                                                                                        aGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           >  1:445750/1‑95 (MQ=255)                                                                                            acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  1:739637/91‑1 (MQ=255)                                                                                            acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           >  2:739637/1‑91 (MQ=255)                                                                                            acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAt                                           >  1:342731/1‑139 (MQ=255)                                                                                             caccacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                          >  2:533129/1‑139 (MQ=255)                                                                                             caccacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                          <  1:580777/139‑1 (MQ=255)                                                                                             caccacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                          <  1:194933/139‑1 (MQ=255)                                                                                              accacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCCCTGTGCTGAACGGTGCCATTg                                         >  2:406239/1‑139 (MQ=255)                                                                                                 acacTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGTCGACGCTGAACGACAGTACCGTCACCGCGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAGCGGTGCCATTGAcc                                      <  1:469308/139‑1 (MQ=255)                                                                                                  cacTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAccc                                     >  1:204706/1‑139 (MQ=255)                                                                                                  cacTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAccc                                     >  1:64243/1‑139 (MQ=255)                                                                                                   acTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAcccc                                    <  2:227506/139‑1 (MQ=255)                                                                                                    cTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCa                                   <  1:533129/139‑1 (MQ=255)                                                                                                      cACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAGCGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACg                                 <  1:362890/139‑1 (MQ=255)                                                                                                       aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                >  1:638325/1‑75 (MQ=255)                                                                                                       aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                <  2:638325/75‑1 (MQ=255)                                                                                                       aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              <  2:39639/109‑1 (MQ=255)                                                                                                       aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                              >  1:39639/1‑109 (MQ=255)                                                                                                        cccAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACa                                                                                                                          >  1:596462/1‑48 (MQ=255)                                                                                                        cccAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACa                                                                                                                          <  2:596462/48‑1 (MQ=255)                                                                                                        cccAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGaa                               >  1:700404/1‑139 (MQ=255)                                                                                                          cAGAAGGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAGGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATg                             <  2:293424/139‑1 (MQ=255)                                                                                                          cAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           <  1:176644/77‑1 (MQ=255)                                                                                                          cAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                           >  2:176644/1‑77 (MQ=255)                                                                                                               aGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCAct                        >  1:140660/1‑139 (MQ=255)                                                                                                               aGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCAct                        >  1:549782/1‑139 (MQ=255)                                                                                                               aGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCAct                        <  1:350779/139‑1 (MQ=255)                                                                                                                 ccGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCActct                      >  2:745490/1‑139 (MQ=255)                                                                                                                 ccGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCActct                      >  2:671229/1‑139 (MQ=255)                                                                                                                  cGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACtctc                     >  2:196564/1‑139 (MQ=255)                                                                                                                   gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGaa                                                    >  1:696652/1‑107 (MQ=255)                                                                                                                   gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGaa                                                    <  2:696652/107‑1 (MQ=255)                                                                                                                   gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGAACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                    >  2:442656/1‑139 (MQ=255)                                                                                                                       aCCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTc                >  1:190296/1‑139 (MQ=255)                                                                                                                           gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt            <  2:342731/139‑1 (MQ=255)                                                                                                                            aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  1:406239/139‑1 (MQ=255)                                                                                                                            aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  2:598175/139‑1 (MQ=255)                                                                                                                            aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  1:431795/139‑1 (MQ=255)                                                                                                                            aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg           <  2:563565/139‑1 (MQ=255)                                                                                                                                aaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCAc       <  2:642518/139‑1 (MQ=255)                                                                                                                                   gCACGCCGACGCTGAACGATAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTg    >  1:589647/1‑139 (MQ=255)                                                                                                                                     acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa  <  1:402396/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGA  >  NC_000913/2072958‑2073228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑