Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,084,773 G→A G251G (GGC→GGT yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,084,7730GA99.0% 307.6 / ‑3.9 98coding (753/1059 nt)yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (44/53);  minor base . (1/0);  total (45/53)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907
                                                                                                                                        |                                                                                                                                      
tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:177506/139‑1 (MQ=255)
tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:772380/139‑1 (MQ=255)
tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:58506/139‑1 (MQ=255)
  gCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    <  2:480660/139‑1 (MQ=255)
    aCCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAg                                                                                                                                  >  1:221263/1‑139 (MQ=255)
     ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAGCACCAGGAAAACACCCCAGt                                                                                                                                 <  2:453532/139‑1 (MQ=255)
     ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  1:388632/139‑1 (MQ=255)
     ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  2:591611/139‑1 (MQ=255)
     ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  1:745822/139‑1 (MQ=255)
       gAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTa                                                                                                                               <  2:318277/139‑1 (MQ=255)
       gAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCGCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTa                                                                                                                               <  2:202115/139‑1 (MQ=255)
        aaacccATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  1:59428/136‑1 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACTTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  2:62033/139‑1 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  1:415596/139‑1 (MQ=255)
           cccATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGt                                                                                                                           <  1:188102/139‑1 (MQ=255)
                   tACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATg                                                                                                                   <  2:678058/139‑1 (MQ=255)
                        caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAAt                                                                                                                             <  2:138371/124‑1 (MQ=255)
                        caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAAt                                                                                                                             >  1:138371/1‑124 (MQ=255)
                        caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                              >  2:696680/1‑139 (MQ=255)
                        caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                              >  1:392295/1‑139 (MQ=255)
                         accTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:732959/114‑1 (MQ=255)
                         accTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:732959/1‑114 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              >  1:421490/1‑121 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  2:421490/121‑1 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            <  1:613126/139‑1 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  2:123795/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            <  2:278747/139‑1 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  2:362439/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:391777/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:94024/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            <  2:752808/139‑1 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:278747/1‑139 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:682102/1‑139 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  1:694480/1‑115 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  2:694480/115‑1 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              >  1:567802/1‑118 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  2:567802/118‑1 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  2:705190/1‑136 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            <  1:705190/136‑1 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGa                                                                                                         <  1:384821/139‑1 (MQ=255)
                                  gCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    >  1:243408/1‑139 (MQ=255)
                                   cTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  2:92767/1‑104 (MQ=255)
                                   cTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:92767/104‑1 (MQ=255)
                                    tACGTACTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACAAGGAAAACACAACAGTTAATGTATTTCATTTCACCCGCGACAAGaaa                                                                                                  >  1:322314/1‑139 (MQ=255)
                                     aCGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    <  1:488365/104‑1 (MQ=255)
                                     aCGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    >  2:488365/1‑104 (MQ=255)
                                         aaTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  2:392295/139‑1 (MQ=255)
                                         aaTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  1:466967/139‑1 (MQ=255)
                                          aTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  1:123795/139‑1 (MQ=255)
                                          aTGGTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  1:497519/139‑1 (MQ=255)
                                                tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                      >  1:459179/1‑139 (MQ=255)
                                                  tgtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   >  2:603248/1‑139 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:8652/1‑88 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:8652/88‑1 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  1:415359/139‑1 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  2:459179/139‑1 (MQ=255)
                                                   gtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   >  2:640599/1‑139 (MQ=255)
                                                      cATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     <  2:388299/86‑1 (MQ=255)
                                                      cATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     >  1:388299/1‑86 (MQ=255)
                                                      cATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   <  2:273772/136‑1 (MQ=255)
                                                      cATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                   >  1:273772/1‑136 (MQ=255)
                                                          aGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTggg                                                                            <  2:525286/139‑1 (MQ=255)
                                                           gCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg                                                                           <  1:547935/139‑1 (MQ=255)
                                                              gcgcGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAg                                                                        >  2:655276/1‑139 (MQ=255)
                                                                       cgGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTa                                                               <  1:640599/139‑1 (MQ=255)
                                                                                       cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               >  2:356665/1‑139 (MQ=255)
                                                                                       cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               >  1:688946/1‑139 (MQ=255)
                                                                                         ttGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCgg                                             <  1:577441/139‑1 (MQ=255)
                                                                                               gCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCtt                                       <  2:243408/139‑1 (MQ=255)
                                                                                                cAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTc                                      >  2:303034/1‑139 (MQ=255)
                                                                                                  aTAAATGACCCCACGAAGATCCCTAATACCAGGAAAACACCCCAGTTAATGAATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCAc                                    >  1:6248/1‑139 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               <  1:680767/120‑1 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               >  2:680767/1‑120 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  1:69987/139‑1 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  2:221263/139‑1 (MQ=255)
                                                                                                            ccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            <  1:120926/57‑1 (MQ=255)
                                                                                                            ccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  2:120926/1‑57 (MQ=255)
                                                                                                             cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 >  2:505611/1‑99 (MQ=255)
                                                                                                             cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 <  1:505611/99‑1 (MQ=255)
                                                                                                               cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGc                       >  1:423967/1‑139 (MQ=255)
                                                                                                                 aaGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTa                     <  2:699112/139‑1 (MQ=255)
                                                                                                                        cTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAgcgc              >  2:210957/1‑139 (MQ=255)
                                                                                                                          aaCACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGCAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCaa            <  1:638259/139‑1 (MQ=255)
                                                                                                                             acCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCa         <  2:423967/139‑1 (MQ=255)
                                                                                                                             acCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCa         >  1:95729/1‑139 (MQ=255)
                                                                                                                              ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg                                                                           <  2:343752/72‑1 (MQ=255)
                                                                                                                              ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg                                                                           >  1:343752/1‑72 (MQ=255)
                                                                                                                              ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaa        >  1:323699/1‑139 (MQ=255)
                                                                                                                              ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaa        >  1:696459/1‑139 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaaa       >  1:678385/1‑139 (MQ=255)
                                                                                                                                aGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAc      >  1:587835/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  1:433255/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  2:701611/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  2:538229/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTAACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTGCTAACCCAAACATGCGTCCGGTTGATTCACTCAGGGGACAGGCTAAAAGCGCAATCAAAca     >  2:486344/1‑138 (MQ=255)
                                                                                                                                  gAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCg    <  2:587835/139‑1 (MQ=255)
                                                                                                                                    aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  2:323699/139‑1 (MQ=255)
                                                                                                                                    aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCATCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  1:518877/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: