Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,085,831 A→G L411P (CTG→CCG)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,8310AG100.0% 461.7 / NA 127L411P (CTG→CCG) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (67/60);  total (67/60)

Only 100 of 127 total aligned reads displayed.

AAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGC  >  NC_000913/2085696‑2085965
                                                                                                                                       |                                                                                                                                      
aaaGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     <  1:688778/139‑1 (MQ=255)
  aGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCa                                                                                                                                   >  1:163197/1‑139 (MQ=255)
   gatgatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCAc                                                                                                                                  >  1:411264/1‑139 (MQ=255)
             cTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                        <  1:623061/139‑1 (MQ=255)
             cTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                        <  1:616656/139‑1 (MQ=255)
               aCGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      <  1:532458/139‑1 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                                     >  1:582178/1‑139 (MQ=255)
                  tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                   <  2:527128/139‑1 (MQ=255)
                  tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                   >  1:416499/1‑139 (MQ=255)
                      cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcaca                                                                                                               >  1:326135/1‑139 (MQ=255)
                        tACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             <  2:163197/139‑1 (MQ=255)
                            gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         <  1:771758/139‑1 (MQ=255)
                             cGGAACCGGATTACGGAAGGTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  1:513836/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             <  1:397447/134‑1 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        <  2:416499/139‑1 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  2:66045/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  1:344724/1‑139 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCACGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGACAGCGCACCACACATCGGc                                                                                                        >  2:193903/1‑139 (MQ=255)
                              ggAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       <  1:665753/139‑1 (MQ=255)
                              ggAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       <  2:472746/139‑1 (MQ=255)
                              ggAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       >  1:181236/1‑139 (MQ=255)
                               gAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAg                                                                                                      >  1:320900/1‑139 (MQ=255)
                                  ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   <  1:318893/139‑1 (MQ=255)
                                  ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   >  2:506455/1‑139 (MQ=255)
                                  ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   >  2:318893/1‑139 (MQ=255)
                                    ggATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                 <  2:344724/139‑1 (MQ=255)
                                              aGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       <  2:255830/123‑1 (MQ=255)
                                               gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTgg                                                                                         <  1:318951/136‑1 (MQ=255)
                                               gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTgg                                                                                         >  2:318951/1‑136 (MQ=255)
                                                ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           >  1:257882/1‑101 (MQ=255)
                                                ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           <  2:257882/101‑1 (MQ=255)
                                                   ttGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGAt                                                                                  <  2:320900/139‑1 (MQ=255)
                                                    tGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        <  1:242937/116‑1 (MQ=255)
                                                    tGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        <  1:362736/116‑1 (MQ=255)
                                                    tGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  2:362736/1‑116 (MQ=255)
                                                            cAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                         <  1:670543/139‑1 (MQ=255)
                                                               gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        <  2:642500/105‑1 (MQ=255)
                                                               gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  1:642500/1‑105 (MQ=255)
                                                                         tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        <  1:697447/95‑1 (MQ=255)
                                                                         tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  2:697447/1‑95 (MQ=255)
                                                                         tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt                                                            >  1:444441/1‑139 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     <  2:345773/62‑1 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     >  1:345773/1‑62 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                               >  2:392725/1‑132 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                               <  1:392725/132‑1 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                        >  1:172680/1‑139 (MQ=255)
                                                                             aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                        >  2:121787/1‑139 (MQ=255)
                                                                              ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         >  2:757242/1‑89 (MQ=255)
                                                                              ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         <  1:757242/89‑1 (MQ=255)
                                                                              ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       >  2:765593/1‑139 (MQ=255)
                                                                                 aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                        >  1:165441/1‑103 (MQ=255)
                                                                                 aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                        <  2:165441/103‑1 (MQ=255)
                                                                                 aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGa                                                    >  1:43007/1‑139 (MQ=255)
                                                                                 aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGa                                                    <  1:748729/139‑1 (MQ=255)
                                                                                  tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                         <  2:76443/117‑1 (MQ=255)
                                                                                  tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                               >  2:588925/1‑127 (MQ=255)
                                                                                  tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                               <  1:588925/127‑1 (MQ=255)
                                                                                   agcagcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgc                                                                                                                       >  1:753172/1‑70 (MQ=255)
                                                                                          cAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   <  2:597568/83‑1 (MQ=255)
                                                                                          cAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   >  1:597568/1‑83 (MQ=255)
                                                                                             aTCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      <  2:540864/61‑1 (MQ=255)
                                                                                             aTCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      >  1:540864/1‑61 (MQ=255)
                                                                                               cAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCgag                                      >  2:392317/1‑139 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     >  1:272269/1‑41 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     <  2:272269/41‑1 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                 <  1:19502/77‑1 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                 >  2:19502/1‑77 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCtt                                                                               >  2:153016/1‑95 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCtt                                                                               <  1:153016/95‑1 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                                   >  1:197236/1‑139 (MQ=255)
                                                                                                  aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACGGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAg                                               <  2:546551/127‑1 (MQ=255)
                                                                                                     cAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGAcc                                >  2:205790/1‑139 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           >  2:685394/1‑46 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           <  1:685394/46‑1 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAg                                                                                                      >  2:190219/1‑67 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                    >  2:164135/1‑69 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCgaga                                     <  2:613681/132‑1 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                              <  1:121787/139‑1 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                              <  2:411264/139‑1 (MQ=255)
                                                                                                       gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                              >  1:309615/1‑139 (MQ=255)
                                                                                                        aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                             <  2:43007/139‑1 (MQ=255)
                                                                                                        aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                             <  2:368843/139‑1 (MQ=255)
                                                                                                        aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                             <  2:277948/139‑1 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAAGCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                            >  2:463830/1‑87 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      <  1:199828/45‑1 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      >  2:199828/1‑45 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                            <  1:463830/87‑1 (MQ=255)
                                                                                                             ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGtt                        >  1:343460/1‑139 (MQ=255)
                                                                                                                  cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      >  2:140341/1‑40 (MQ=255)
                                                                                                                  cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      <  1:140341/40‑1 (MQ=255)
                                                                                                                    ttcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                               >  1:189460/1‑125 (MQ=255)
                                                                                                                    ttcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                               <  2:189460/125‑1 (MQ=255)
                                                                                                                      cttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGt               >  2:39255/1‑139 (MQ=255)
                                                                                                                               tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccac      >  2:747774/1‑139 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                         <  1:415472/70‑1 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                         >  2:415472/1‑70 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAAc                                            >  2:314094/1‑99 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca    >  2:205606/1‑139 (MQ=255)
                                                                                                                                 aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca    >  2:507069/1‑139 (MQ=255)
                                                                                                                                  cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg   <  1:56223/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGC  >  NC_000913/2085696‑2085965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: