Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,090,046 G→A *17* (TAG→TAA hisL → his operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,0460GA100.0% 443.7 / NA 135*17* (TAG→TAAhisLhis operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (67/68);  total (67/68)

Only 100 of 135 total aligned reads displayed.

TATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAG  >  NC_000913/2089915‑2090179
                                                                                                                                   |                                                                                                                                     
tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                >  1:387362/1‑139 (MQ=255)
tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                >  2:336059/1‑139 (MQ=255)
tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                >  1:250667/1‑139 (MQ=255)
tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                >  2:714122/1‑139 (MQ=255)
tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCATGACTAATCTTTCa                                                                                                                                >  2:645407/1‑139 (MQ=255)
  taAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                              <  2:218713/139‑1 (MQ=255)
  taAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                              <  2:391200/139‑1 (MQ=255)
   aaaaaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGc                                                                                                                             >  2:664085/1‑139 (MQ=255)
      aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                          <  1:656245/139‑1 (MQ=255)
        aGCCCTTGCTTTCTAACGTGAATGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTCTTATGACACGCGTTCAATTTAAACACCACCATCCTCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                        >  2:717426/1‑139 (MQ=255)
        aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                        >  1:320406/1‑139 (MQ=255)
        aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                         <  2:35231/138‑1 (MQ=255)
          cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                      >  1:313616/1‑139 (MQ=255)
          cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                      >  1:645992/1‑139 (MQ=255)
               gCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTgg                                                                                                                 <  2:26039/139‑1 (MQ=255)
                 tttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                               <  1:618217/139‑1 (MQ=255)
                  ttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                              <  2:256016/139‑1 (MQ=255)
                           gAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                     <  2:410363/139‑1 (MQ=255)
                            aaaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                          >  1:523129/1‑117 (MQ=255)
                            aaaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                          <  2:523129/117‑1 (MQ=255)
                                tGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTc                                                                                                <  1:12352/139‑1 (MQ=255)
                                  gTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCa                                                                                              >  1:338296/1‑139 (MQ=255)
                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             <  2:645992/139‑1 (MQ=255)
                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             <  1:50661/139‑1 (MQ=255)
                                          tAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             <  2:153945/132‑1 (MQ=255)
                                          tAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             >  1:153945/1‑132 (MQ=255)
                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                  >  2:348171/1‑93 (MQ=255)
                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                  <  1:348171/93‑1 (MQ=255)
                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                    <  1:226245/139‑1 (MQ=255)
                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                    >  2:392678/1‑139 (MQ=255)
                                              aGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                  <  2:320406/139‑1 (MQ=255)
                                                 cATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGc                                                                               <  2:531203/139‑1 (MQ=255)
                                                  aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTACACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCa                                                                              >  2:390438/1‑139 (MQ=255)
                                                  aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                       <  2:616533/114‑1 (MQ=255)
                                                  aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                       >  1:616533/1‑114 (MQ=255)
                                                  aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCa                                                                              >  1:59859/1‑139 (MQ=255)
                                                  aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCa                                                                              >  1:35597/1‑139 (MQ=255)
                                                  aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCa                                                                              >  2:745494/1‑139 (MQ=255)
                                                       ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                         <  1:357339/139‑1 (MQ=255)
                                                       ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                         <  1:430313/139‑1 (MQ=255)
                                                         gAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaaa                                                                       >  1:730500/1‑139 (MQ=255)
                                                          aaTAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAg                                                                      >  2:489781/1‑139 (MQ=255)
                                                            tAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                  <  2:684395/125‑1 (MQ=255)
                                                            tAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                  >  1:684395/1‑125 (MQ=255)
                                                              aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGcccc                                                                  <  2:723025/139‑1 (MQ=255)
                                                                 aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             <  2:91534/109‑1 (MQ=255)
                                                                 aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             >  1:91534/1‑109 (MQ=255)
                                                                 aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGc                                                                                       >  2:169476/1‑115 (MQ=255)
                                                                    cacaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaaa                                                                       >  1:162354/1‑128 (MQ=255)
                                                                    cacaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaaa                                                                       <  2:162354/128‑1 (MQ=255)
                                                                        gagaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             >  2:439297/1‑102 (MQ=255)
                                                                         agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAt                                                                             >  1:117468/1‑117 (MQ=255)
                                                                         agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAt                                                                             <  2:117468/117‑1 (MQ=255)
                                                                         agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                       >  2:587434/1‑139 (MQ=255)
                                                                          gaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAcc                                                      >  1:475119/1‑139 (MQ=255)
                                                                          gaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAcc                                                      <  1:71090/139‑1 (MQ=255)
                                                                              tttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                  <  2:730500/139‑1 (MQ=255)
                                                                                 aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCTGGAAGATCACCTTCCggg                                               <  1:507200/139‑1 (MQ=255)
                                                                                     cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGa                                                                                   >  1:394928/1‑99 (MQ=255)
                                                                                     cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                           >  1:56967/1‑139 (MQ=255)
                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTc                                                                                                <  1:463034/84‑1 (MQ=255)
                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTc                                                                                                >  2:463034/1‑84 (MQ=255)
                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                         >  1:100022/1‑139 (MQ=255)
                                                                                                tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                <  2:718759/139‑1 (MQ=255)
                                                                                                tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                <  1:489781/139‑1 (MQ=255)
                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                       <  1:159582/139‑1 (MQ=255)
                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                       >  2:633365/1‑139 (MQ=255)
                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                       >  1:433777/1‑139 (MQ=255)
                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                    <  1:427361/77‑1 (MQ=255)
                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                    >  2:427361/1‑77 (MQ=255)
                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAg                                                                      >  1:622390/1‑91 (MQ=255)
                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                             <  2:401021/116‑1 (MQ=255)
                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                      >  2:69955/1‑139 (MQ=255)
                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATATTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                             >  1:401021/1‑116 (MQ=255)
                                                                                                              tcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                <  1:53778/45‑1 (MQ=255)
                                                                                                                  caccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa             <  2:338296/139‑1 (MQ=255)
                                                                                                                   accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAgtg                                                                                           >  1:277866/1‑61 (MQ=255)
                                                                                                                   accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa             >  2:276157/1‑139 (MQ=255)
                                                                                                                   accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa             <  2:56967/139‑1 (MQ=255)
                                                                                                                   accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa             <  2:35597/139‑1 (MQ=255)
                                                                                                                      atcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                        <  2:314610/45‑1 (MQ=255)
                                                                                                                      atcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                        >  1:314610/1‑45 (MQ=255)
                                                                                                                      atcagcacgACTAATCTTTAAGGCGATGGGTGCTGGAAGACCTGCAGATCTTCCGCTGGACGATGGACGCCTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGACTTTTTTCTTACGCAGATGATAACCGTCCAGACAg          >  2:149799/9‑139 (MQ=255)
                                                                                                                       tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc                                            <  2:588648/104‑1 (MQ=255)
                                                                                                                       tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc                                            >  1:588648/1‑104 (MQ=255)
                                                                                                                         atcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                               <  2:536867/99‑1 (MQ=255)
                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                             >  1:722090/1‑52 (MQ=255)
                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGc                                                                               <  1:56778/66‑1 (MQ=255)
                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGc                                                                               >  2:56778/1‑66 (MQ=255)
                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt      >  2:608344/1‑139 (MQ=255)
                                                                                                                           ccTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                 >  1:89190/1‑95 (MQ=255)
                                                                                                                           ccTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                 <  2:89190/95‑1 (MQ=255)
                                                                                                                            cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa    >  2:612596/1‑139 (MQ=255)
                                                                                                                            cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa    <  1:69955/139‑1 (MQ=255)
                                                                                                                              gACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag  <  1:699802/139‑1 (MQ=255)
                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGTCATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg                    >  2:303392/1‑120 (MQ=255)
                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGcccc                                                                  <  1:586672/74‑1 (MQ=255)
                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGcccc                                                                  >  2:586672/1‑74 (MQ=255)
                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg                    <  1:303392/120‑1 (MQ=255)
                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag  <  1:767088/138‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
TATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAG  >  NC_000913/2089915‑2090179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: