Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,115,907 C→T *407* (TAG→TAA wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,9070CT100.0% 71.2 / NA 23*407* (TAG→TAAwcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

TGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCC  >  NC_000913/2115771‑2116033
                                                                                                                                        |                                                                                                                              
tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTta                                                                                                                              >  1:753379/1‑139 (MQ=255)
 gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTtat                                                                                                                             <  2:129423/139‑1 (MQ=255)
                     aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                         <  2:56067/139‑1 (MQ=255)
                      aCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCg                                                                                                        >  1:277677/1‑139 (MQ=255)
                         cTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGt                                                                                                     <  2:614681/139‑1 (MQ=255)
                             gTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 <  1:4223/139‑1 (MQ=255)
                               cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                                                               <  2:35681/139‑1 (MQ=255)
                                gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                                                              <  2:636151/139‑1 (MQ=255)
                                          cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                    <  1:690554/139‑1 (MQ=255)
                                          cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                    <  1:716899/139‑1 (MQ=255)
                                          cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                    <  2:330777/139‑1 (MQ=255)
                                            cgcgGGCGAAAGGGGTATGGAGGAAGGCGAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTg                                                                                                                     >  1:478042/1‑104 (MQ=255)
                                            cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTg                                                                                                                     <  2:478042/104‑1 (MQ=255)
                                            cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 >  1:298499/1‑124 (MQ=255)
                                            cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 <  2:298499/124‑1 (MQ=255)
                                                         ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                                                     <  1:715638/139‑1 (MQ=255)
                                                                     ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 <  1:158213/99‑1 (MQ=255)
                                                                     ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 >  2:158213/1‑99 (MQ=255)
                                                                          gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcg                                                    >  2:90478/1‑139 (MQ=255)
                                                                                                    cGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 >  2:158280/1‑68 (MQ=255)
                                                                                                    cGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                 <  1:158280/68‑1 (MQ=255)
                                                                                                                         ggCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAAc     >  1:753958/1‑139 (MQ=255)
                                                                                                                            aTGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAAcgcc  >  2:727548/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                              
TGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCC  >  NC_000913/2115771‑2116033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: