Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,131,667 T→C D135G (GAC→GGC)  wcaC ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,131,6670TC100.0% 71.1 / NA 22D135G (GAC→GGC) wcaCputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/14);  total (8/14)

CCCGCCACCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTGCGAG  >  NC_000913/2131535‑2131802
                                                                                                                                    |                                                                                                                                       
cccGCCACCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTATCACTCCAGTGGCCGTGCa                                                                                                                                   <  2:428191/139‑1 (MQ=255)
              gtgtGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTccagaccaga                                                                                                                     <  2:416136/139‑1 (MQ=255)
               tgtgCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCagag                                                                                                                    >  2:451256/1‑139 (MQ=255)
                     cgATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGt                                                                                                              <  2:73987/139‑1 (MQ=255)
                                      cggATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTc                                                                                             >  2:195065/1‑139 (MQ=255)
                                                     ggTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAg                                                                              >  2:1886/1‑139 (MQ=255)
                                                           gCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTc                                                                                                                              <  2:350632/85‑1 (MQ=255)
                                                           gCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTc                                                                                                                              >  1:350632/1‑85 (MQ=255)
                                                            cATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacca                                                                       <  1:451256/139‑1 (MQ=255)
                                                             aTTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCATCGTCCGGTAACTCTCCAGTGGCCGTTCAGCGTCCAGGCAAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGcacgac                                                                      <  2:392226/139‑1 (MQ=255)
                                                                           gTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTa                                                        <  2:770549/139‑1 (MQ=255)
                                                                           gTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTa                                                        >  2:44071/1‑139 (MQ=255)
                                                                              ttCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGcc                                                     <  2:222850/139‑1 (MQ=255)
                                                                                   gCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAg                                                <  2:357352/139‑1 (MQ=255)
                                                                                   gCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAg                                                <  1:554447/139‑1 (MQ=255)
                                                                                          cAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTgca                                         <  2:749867/139‑1 (MQ=255)
                                                                                                                         cacTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAgcg                                                                            <  1:74909/73‑1 (MQ=255)
                                                                                                                         cacTCCAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAgcg                                                                            >  2:74909/1‑73 (MQ=255)
                                                                                                                              cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTGc     >  2:499464/1‑139 (MQ=255)
                                                                                                                              cAGTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTGc     <  1:467664/139‑1 (MQ=255)
                                                                                                                                gTGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTGCGa   >  1:562011/1‑139 (MQ=255)
                                                                                                                                 tGGCCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTGCGAg  <  1:44071/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
CCCGCCACCAGTTGGTGTGCGCGATCAATCTTCACCGGCGGATAGTTATTTAAGGTCGGGCATTTCTGGCAGCCTGTTTTCCAGCCTTCGCAACCGTCGGTAAAGGCGCAGCGTCCGGTAACACTCCAGTGGTCGTGCAGCGTCCAGACCAGAGTGACGTCCGGTTTATGGTTTTTCACTTTTTCGCAAAAGCGCACCACGCTCTTAAGATTTAGCCAGTAGCTGTGCAGCACATGAAAATGCAGGACCACCGGACCCGCTGTGCGAG  >  NC_000913/2131535‑2131802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: