Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,153,502 A→G intergenic (+51/‑142) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,5020AG100.0% 143.6 / NA 41intergenic (+51/‑142)sibA/sibBsRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/16);  total (25/16)

AATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGG  >  NC_000913/2153367‑2153630
                                                                                                                                       |                                                                                                                                
aaTCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGc                                                                                                                               <  2:478229/139‑1 (MQ=255)
aaTCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGc                                                                                                                               >  2:475397/1‑139 (MQ=255)
        agagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATc                                                                                                                       >  1:2978/1‑139 (MQ=255)
             aTCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTaa                                                                                                                  >  1:709726/1‑139 (MQ=255)
              tCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAAc                                                                                                                 >  2:3845/1‑139 (MQ=255)
                aCCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAAcgc                                                                                                               <  2:709726/139‑1 (MQ=255)
                    gTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGa                                                                                                           >  1:606840/1‑139 (MQ=255)
                    gTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGa                                                                                                           >  2:102865/1‑139 (MQ=255)
                       tgatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATa                                                                                                        <  1:391232/139‑1 (MQ=255)
                              gTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGt                                                                                                 >  1:271244/1‑139 (MQ=255)
                               tGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGtt                                                                                                >  1:309029/1‑139 (MQ=255)
                               tGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGtt                                                                                                >  1:532955/1‑139 (MQ=255)
                               tGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGtt                                                                                                <  2:2978/139‑1 (MQ=255)
                                 cTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTc                                                                                              >  2:372132/1‑139 (MQ=255)
                                     atcatAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTAAATAAAATAGTTTccctg                                                                                          >  2:276569/1‑136 (MQ=255)
                                          aaCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGat                                                                                     <  1:681388/139‑1 (MQ=255)
                                            cccTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatta                                                                                   >  2:29358/1‑139 (MQ=255)
                                                 tCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCg                                                                              >  1:381472/1‑139 (MQ=255)
                                                  ccTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGt                                                                             >  1:748928/1‑139 (MQ=255)
                                                             gCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCa                                                                  <  2:532955/139‑1 (MQ=255)
                                                                 tcttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGaa                                                                                                          <  2:645133/95‑1 (MQ=255)
                                                                 tcttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGaa                                                                                                          >  1:645133/1‑95 (MQ=255)
                                                                  cttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGaa                                                             >  2:437328/1‑139 (MQ=255)
                                                                              agGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCa                                                 <  1:102330/139‑1 (MQ=255)
                                                                               ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTcc                                                                                             <  2:110272/94‑1 (MQ=255)
                                                                               ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTcc                                                                                             >  1:110272/1‑94 (MQ=255)
                                                                                gCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatta                                                                                   >  2:415068/1‑103 (MQ=255)
                                                                                gCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatta                                                                                   <  1:415068/103‑1 (MQ=255)
                                                                                gCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAgg                                               >  1:526790/1‑139 (MQ=255)
                                                                                 cTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGa                                              <  1:29358/139‑1 (MQ=255)
                                                                                      ccGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCa                                         <  2:526790/139‑1 (MQ=255)
                                                                                                 tCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCtg                                                                                         >  2:600896/1‑80 (MQ=255)
                                                                                                 tCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCtg                                                                                         <  1:600896/80‑1 (MQ=255)
                                                                                                    cGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGtt                                                                                                >  2:746491/1‑70 (MQ=255)
                                                                                                    cGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGtt                                                                                                <  1:746491/70‑1 (MQ=255)
                                                                                                            tcatcGGCTTACCACCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGa                   <  2:124902/139‑1 (MQ=255)
                                                                                                              atcGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACa                 >  1:98345/1‑139 (MQ=255)
                                                                                                                cGGCTTACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAAt               >  2:650954/1‑139 (MQ=255)
                                                                                                                     tACCTCCTTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTAt          >  1:447571/1‑139 (MQ=255)
                                                                                                                         tcctTTCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTg      <  1:626545/139‑1 (MQ=255)
                                                                                                                             ttCGCCCTGAGTGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAgg  >  2:42669/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                
AATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGG  >  NC_000913/2153367‑2153630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: